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Translocation of c-abl oncogene and PDGFB (c-sis) gene in a case of CML with 46, XY, t(22;22)

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In a case of CML with a variant Philadelphia translocation (Ph1 or Ph) t (22;22) (q11;q13) in bone marrow cells and unstimulated peripheral blood cells, no cytogenetically detectable involvement of chromosome 9 was observed. Southern blot experiments using probes specific for bcr and c-sis however revealed rearrangement of the bcr, but not of PDGFB (c-sis) gene. Northern blot analysis of bone marrow RNA showed a very weak signal with the c-sis probe, while in a lymph-node biopsy PDGFB m-RNA could not be detected. Chromosomal in situ hybridization gave evidence for translocation of c-abl from chromosome 9 to Ph and of PDGFB from chromosome 22 to chromosome 9, as the result of a threefold translocation t(9;22;22).

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Authors and Affiliations

  1. Institut für Humangenetik, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck 1, Federal Republic of Germany

    G. Gradl & C. Fonatsch

  2. Medizinische Universitätsklinik I, Josef Stelzmannstrasse 9, D-5000, Köln 41, Federal Republic of Germany

    H. Tesch

  3. Klinik für Innere Medizin, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400, Lübeck 1, Federal Republic of Germany

    G. Schwieder & T. Wagner

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  4. T. Wagner
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  5. C. Fonatsch
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Gradl, G., Tesch, H., Schwieder, G. et al. Translocation of c-abl oncogene and PDGFB (c-sis) gene in a case of CML with 46, XY, t(22;22). Blut 58, 279–285 (1989). https://doi.org/10.1007/BF00320166

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