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Change in the chemiluminescence reactivity pattern during in vitro differentiation of human monocytes to macrophages

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Summary

We determined the luminol-enhanced chemiluminescence (CL) of fresh human monocytes and monocytes cultured for 1–14 days in vitro, within hydrophobic membranes, using a variety of stimuli known to trigger the respiratory burst of phagocytes. It was assured that CL emerged from an adherent subpopulation of mononuclear cells; polymorphonuclear leukocytes (PMN) contaminating mononuclear leukocytes (MNL) contributed little, if anything, to the CL response of MNL. Typical response patterns were established for fresh monocytes triggered by phorbol 12-myristate 13-acetate (PMA), zymosan, the Ca2+ ionophore A 23187, antibody-coated erythrocytes and Sendai virus. Differentiation in vitro into macrophages was associated with a general decrease in magnitude of the CL peak, in an overproportional decrease of the A 23187 triggered response and in a complete loss of the response to Sendai virus — a loss which could not be prevented by addition of myeloperoxidase (MPO). In contrast to monocyte CL, macrophage CL was resistant to sodium azide, indicating its MPO-independent origin. Macrophage-type reactivity was obtained at day 4 of culture. Activation of macrophages with recombinant interferon-γ for the last 2 days of culture was associated with a quantitative (approx. threefold) increase of the CL signal, although qualitatively the same reactivity pattern was obtained as with control macrophages. In contrast to luminol-dependent CL, the lucigenin-dependent CL response of macrophages was greater than that of monocytes, an increase which was particularly prominent for PMA stimulation.

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Authors and Affiliations

  1. Institute of Veterinary Virology, and Institute of Clinical and Experimental Cancer Research, University of Berne, Länggasstrasse 122, CH-3012, Berne, Switzerland

    Thomas W. Jungi & Ernst Peterhans

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  1. Thomas W. Jungi
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  2. Ernst Peterhans
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Part of this work has been presented at the 4th International Meeting on Chemiluminescence and Bioluminescence (Freiburg, FRG, Sept. 86) and at the 23rd National Meeting of the Reticuloendothelial Society (Denver, Sept. 86)

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Jungi, T.W., Peterhans, E. Change in the chemiluminescence reactivity pattern during in vitro differentiation of human monocytes to macrophages. Blut 56, 213–220 (1988). https://doi.org/10.1007/BF00320108

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  • DOI: https://doi.org/10.1007/BF00320108

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