Skip to main content
SpringerLink
Log in

Morphological and chromosomal characterization of three new continuous cell lines of Drosophila melanogaster

  • Published:
Chromosoma Aims and scope Submit manuscript

Abstract

Three continuous cell lines (GM1, GM2 and GM3) were obtained from embryos of Drosophila melanogaster. Karyotypic analysis revealed characteristics distinguishing each line. Except for some minor variations GM1 cells had an X and a centric heterochromatic fragment (which is a portion of the Y). GM2 line was characterized by XO cells showing two “new” telocentric chromosomes while an autosome of the II pair was missing. GM3 cells were XY; the Y chromosome, however, was shorter than the normal, having a deletion of the terminal section of the short arm. Several problems concerning the origin of these different genomes are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Dolfini, S.: Karyotype polymorphism in a cell population of Drosophila melanogaster cultured in vitro. Chromosoma (Berl.) 33, 196–208 (1971).

    Google Scholar 

  • Dolfini, S.: Further data on karyotype polymorphism of Drosophila melanogaster cells cultured in vitro. IIId Intern. Colloquium on Invertebrate Tissue Cult., Smolenice 1971 (in press, 1972).

  • Dolfini, S., Gottardi, A.: Changes of chromosome number in cells of Drosophila melanogaster cultured in vitro. Experientia (Basel) 22, 144 (1966).

    Google Scholar 

  • Echalier, G., Ohanessian, A.: Isolement, en cultures in vitro, de lignées cellulaires diploïdes de Drosophila melanogaster. C. R. Acad. Sci. (Paris) 268, 1771–1773 (1969).

    Google Scholar 

  • Echalier, G., Ohanessian, A.: In vitro culture of Drosophila melanogaster embryonic cells. In Vitro 6, 162–172 (1970).

    Google Scholar 

  • Kakpakov, V. T., Gvozdev, V. A., Platova, T. P., Polukarova, L. G.: In vitro establishment of embryonic cell lines of Drosophila melanogaster. Genetika (USSR) 5, 67–75 (1969).

    Google Scholar 

  • Pearson, P. L., Bobrow, M., Vosa, C. G.: Technique for identifying Y chromosomes in human interphase nuclei. Nature (Lond.) 226, 78–80 (1970).

    Google Scholar 

  • Schneider, I.: Embryonic cell lines of D. melanogaster. Dros. Inf. Ser. 46, 111 (1971).

    Google Scholar 

  • Vosa, C. G.: The discriminating fluorescence patterns of the chromosomes of Drosophila melanogaster. Chromosoma (Berl.) 31, 446–451 (1970).

    Google Scholar 

  • Zuffardi, O., Tiepolo, L., Dolfini, S., Barigozzi, C., Fraccaro, M.: Changes in the fluorescence patterns of translocated Y chromosome segments in Drosophila melanogaster. Chromosoma (Berl.) 34, 274–280 (1971).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics, University of Milano, Italy

    Giuliana Mosna & Silvana Dolfini

Authors
  1. Giuliana Mosna
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Silvana Dolfini
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by a grant of the Consiglio Nazionale delle Ricerche, Roma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mosna, G., Dolfini, S. Morphological and chromosomal characterization of three new continuous cell lines of Drosophila melanogaster . Chromosoma 38, 1–9 (1972). https://doi.org/10.1007/BF00319954

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00319954

Keywords

Search

Navigation