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Autoimmune hemolytic anemia by coexisting Anti-I and Anti-Fl cold agglutinins

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Summary

In association with atypical pneumonia, a patient developed acute severe autoimmune hemolytic anemia. Hemoglobin temporarily was only 7.0 g/100 ml, so that the patient needed red blood cell (RBC) transfusion. Hemolysis was found to be caused by high titer cold agglutinins (CA), which occurred transiently during the acute period of the disease. CA of two different specificities, anti-I and anti-Fl, were demonstrated in the patient's serum. Antibodies of the two specificities were clearly separated by absorption/elution experiments using neuraminidase (RDE)-treated RBC. They were distinguished by serologic means: Both anti-I and anti-Fl react more strongly with adult RBC than with newborn and i adult RBC; in contrast to anti-I, anti-Fl does not agglutinate RDE-treated cells. Inhibition experiments showed that I-active substances prepared from papainized RBC exhibited both I and Fl antigenic activity. By RDE-treatment of I-active substances, Fl-activity was markedly reduced, while I-activity was increased.

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Authors and Affiliations

  1. Institute for Immunology and Serology, University of Heidelberg, Im Neuenheimer Feld 305, D-6900, Heidelberg, Federal Republic of Germany

    A. L. König & D. Roelcke

  2. Medical Clinic, University of Heidelberg, Bergheimer Straße 58, D-6900, Heidelberg, Federal Republic of Germany

    H. Kather

Authors
  1. A. L. König
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  2. H. Kather
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  3. D. Roelcke
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König, A.L., Kather, H. & Roelcke, D. Autoimmune hemolytic anemia by coexisting Anti-I and Anti-Fl cold agglutinins. Blut 49, 363–368 (1984). https://doi.org/10.1007/BF00319883

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  • DOI: https://doi.org/10.1007/BF00319883

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