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A human B lymphocyte antigen (P-76) shared by B-cell chronic lymphocytic leukemia cells and hairy cell leukemia cells

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Summary

A membrane antigen with an apparent specificity to B lymphocytes was detected with immunochemical techniques and its properties were analyzed. Anti-B-CLL serum was raised in a rabbit by immunization with B-cell chronic lymphocytic leukemia (B-CLL) cells. This anti-B-CLL serum was absorbed with erythrocytes, liver homogenate and insolubilized immunoglobulins. After further absorption with T-CLL cells, chronic myelocytic leukemia (CML) cells and acute myelocytic leukemia (AML) cells, the anti-B-CLL serum still reacted with peripheral blood B lymphocytes, B-CLL cells and hairy cell leukemia (HCL) cells. In contrast, no reactivity was seen with peripheral blood T lymphocyte or monocytes, or leukemia cells of non-B cell origin. An immunoprecipitation of radiolabeled cell surface proteins was attempted using the anti-B-CLL serum in the presence of Staphylococcus Aureus Cowan 1 (SaCl), and the precipitates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). A membrane antigen with an apparent molecular weight of 76,000 daltons (P-76) was immunoprecipitated with the anti-B-CLL serum from the lysates of normal B lymphocyte, B-CLL cells and HCL cells. The antigen (P-76) is not composed of disulfide-linked subunits and has no structural relationship with HLA-DR (Ia-like) antigens or other known antigens. These results suggest that this antigen is B-lymphocyte specific, and favour the B-lymphocyte nature of HCL cells.

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Authors and Affiliations

  1. 2nd Department of Internal Medicine, Osaka University Medical School, Fukushima-ku, 553, Osaka, Japan

    Yuzuru Kanakura, Yoshiyuki Kurata, Toshiharu Tamaki, Takeshi Yonezawa & Seiichiro Tarui

  2. Division of Internal Medicine, Department of Clinical Research, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

    Takashi Machii & Teruo Kitani

Authors
  1. Yuzuru Kanakura
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  2. Takashi Machii
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  3. Yoshiyuki Kurata
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  4. Toshiharu Tamaki
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  5. Takeshi Yonezawa
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  6. Seiichiro Tarui
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  7. Teruo Kitani
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Kanakura, Y., Machii, T., Kurata, Y. et al. A human B lymphocyte antigen (P-76) shared by B-cell chronic lymphocytic leukemia cells and hairy cell leukemia cells. Blut 46, 249–259 (1983). https://doi.org/10.1007/BF00319865

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  • DOI: https://doi.org/10.1007/BF00319865

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