Summary
The olfactory epithelium of the three-spined stickleback (Gasterosteus aculeatus) and the nine-spined stickleback (Pungitius pungitius) has been studied with a conventional histochemical and a novel immunological staining technique. In both species, the sensory epithelium is arranged in folds separated by non-sensory epithelial tissue. In the nine-spined stickleback, intrinsic folds consisting of non-sensory cells are found in the apical part of the sensory epithelium where they divide the surface of the sensory epithelium into small islets. These non-sensory cells are non-ciliated, flattened and piled on top of each other; they contain numerous electron-translucent vesicles. The intrinsic folds are absent from the sensory epithelium of the three-spined stickleback. In both species, axons of receptor cells form a layer of fibers in the sensory epithelium immediately above the basal cells. In the three-spined stickleback, thick branches of the olfactory nerve are frequently found in this layer. These branches are only occasionally observed in the sensory epithelium of the nine-spined stickleback. Thus, the three-spined stickleback and the nine-spined stickleback show considerable differences in the organization of the sensory regions of the olfactory epithelium.
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References
Bannister LH (1965) The fine structure of the olfactory surface of teleostean fishes. Q J Microsc Sci 106:333–342
Bertmar G (1973) Ultrastructure of the olfactory, mucosa in the homing Baltic sea trout, Salmo trutta trutta. Marine Biology 19:74–88
Coad BW (1981) A bibliography of the sticklebacks (Gasterosteidae: Osteichthyes). Syllogeus 35:1–142
Elofsson R, Hallberg E (1973) Correlation of ultrastructure and chemical composition of crustacean chromatophore pigment. J Ultrastruct Res 44:421–429
Getchell MI, Zielinski B, Getchell TV (1986) Ontogeny of the secretory elements in the vertebrate olfactory mucosa. In: Breipohl W (ed) Ontogeny of olfaction. Springer, Berlin Heidelberg New York, pp 71–82
Hallberg E, Reschke M (1990) The ultrastructural characteristics of the pheromone gland cells of Dendrolimus pini (Insecta: Lepidoptera). Acta Zool (Stockh) 71:169–172
Holl A (1965) Vergleichende morphologische und histologische Untersuchungen am Geruchsorgan der Knochenfische. Z Morphol Ökol Tiere 54:707–782
Honkanen T, Ekström P (1990) An immunocytochemical study of the olfactory projections in the three-spined stickleback, Gasterosteus aculeatus L. J Comp Neurol 292:65–72
Honkanen T, Ekström P (1991) An immunocytochemical study of the development of the olfactory system in the three-spined stickleback (Gasterosteus aculeatus L., Teleostei). Anat Embryol 184:469–477
Hudon J, Guderley H (1984) An electrophoretic study of the phylogenetic relationships among four species of sticklebacks (Pisces: Gasterosteidae). Can J Zool 62:2313–2316
Lewis DH, Tarpley RJ, Marks JE, Sis RF (1985) Drug induced structural changes in olfactory organ of channel catfish, Ictalurus punctatus, Rafinesque. J Fish Biol 26:355–358
Liermann K (1933) Über den Bau des Geruchsorgans der Teleostier. Z Anat Entwicklungsgesch 100:1–39
McLennan DA, Brooks DR, McPhail JD (1988) The benefits of communication between comparative ethology and phylogenetic systematics: a case study using gasterosteid fishes. Can J Zool 66:2177–2190
Penczak T (1961) Serological relationships between the nine-spined and the three-spined stickleback. Nature 192:673–674
Schulte E, Holl A (1971) Feinstruktur des Riechepithels von Calamoichthys calabaricus JA Smith (Pisces, Brachiopterygii). Z Zellforschung 120:261–279
Teichmann H (1954) Vergleichende Untersuchungen an der Nase der Fische. Z Morphol Ökol Tiere 43:171–212
Theisen B (1982) Functional morphology of the olfactory organ in Spinachia spinachia (L.) (Teleostei, Gasterosteidae). Acta Zool (Stockh) 63:247–254
Theisen B (1984) On the morphology of the olfactory organs in the Gasterosteidae (Teleostei) and related families. Videnskabelige meddelelser fra dansk naturhistorisk forening 145:87–96
Thommesen G (1983) Morphology, distribution and specificity of olfactory receptor cells in salmonid fishes. Acta Physiol Scand 117:241–249
Wootton RJ (1976) The biology of the sticklebacks. Academic Press, London New York San Francisco
Yamamoto M (1982) Comparative morphology of the peripheral olfactory organ in teleosts. In: Hara TJ (ed) Chemoreception in fishes. Elsevier, Amsterdam, pp 39–59
Yamamoto M, Ueda K (1978) Comparative morphology of fish olfactory epithelium. V. Gasterosteiformes, Channiformes and Synbranchiformes. Bull Jpn Soc Sci Fish 44:1309–1314
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Honkanen, T., Ekström, P. Comparative study of the olfactory epithelium of the three-spined stickleback (Gasterosteus aculeatus) and the nine-spined stickleback (Pungitius pungitius). Cell Tissue Res 269, 267–273 (1992). https://doi.org/10.1007/BF00319617
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DOI: https://doi.org/10.1007/BF00319617