Summary
The in vivo effects of tunicamycin on the glycosylation of proteoglycans and link protein in rat mandibular condylar chondrocytes were studied by ultrastructural lectin histochemistry. The binding of wheatgerm agglutinin was shown by using anti-lectin antibody followed by protein A-gold complex. In normal rats, wheat-germ agglutinin labeling was restricted to trans cisternae and vacuoles of the Golgi complex, whereas it was observed in neither the cis region of the Golgi complex nor in the rough endoplasmic reticulum. By 3 h after the drug administration, wheat-germ agglutinin binding sites on the disorganized Golgi vacuoles were dramatically reduced in number. At 6 h after the drug administration, the lectin binding sites on the Golgi vacuoles were restored. These results demonstrate that the in vivo use of tunicamycin in combination with histochemical analysis using lectin probes is of significant value for the study of protein glycosylation in chondrocytes of the rat mandibular condyle.
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References
Burton-Wurster N, Horn VJ, Lust G (1988) Immunohistochemical localization of fibronectin and chondronectin in canine articular cartilage. J Histochem Cytochem 36: 581–588
Chardin H, Septier D, Lecolle S, GoldbergM (1989) Effect of tunicamycin on glycogen accumulation in the stratum intermedium and odontoblasts of rat incisor. Cell Tissue Res 256: 519–527
Choi HU, Tang L-H, Johnson TL, Rosenberg L (1985) Proteoglycans from bovine nasal and articular cartilages. Fractionation of the link proteins by wheat germ agglutinin affinity chromatography. J Biol Chem 260: 13370–13376
Duskin D, Bornstein P (1977) Impaired conversion of procollagen to collagen by fibroblasts and bone treated with tunicamycin, an inhibitor of protein glycosylation. J Biol Chem 252: 955–962
Duskin D, Holbrook K, Williams K, Bornstein P (1978) Cell surface morphology and adhesive properties of normal and virally transformed cells treated with tunicamycin, an inhibitor of protein glycosylation. Exp Cell Res 116: 153–165
Elbein AD (1981) The tunicamycins-useful tools for studies on glycoproteins. Trends Biochem Sci 6: 219–221
González CB, Swann RW, Pickering BT (1981) Effects of tunicamycin on the hypothalamo-neurohypophysial system of the rat. Cell Tissue Res 217: 199–210
Hart GW, Lennarz WJ (1978) Effects of tunicamycin on the biosynthesis of glycosaminoglycans by embryonic chick cornea. J Biol Chem 253: 5795–5801
Hascall VC, Hascall GK (1981) Proteoglycans. In: Hay ED (ed) Cell biology of extracellular matrix. Plenum Press, New York London, pp 39–63
Livne E, Silbermann M (1990) The mouse mandibular condyle: an investigative model in developmental biology. J Cranio Genet Dev Biol 10: 95–98
Lohmander LS, Fellini SA, Kimura JH, Stevens RL, Hascall VC (1983) Formation of proteoglycan aggregates in rat chondrosarcoma chondrocyte cultures treated with tunicamycin. J Biol Chem 258: 12280–12286
Marchi F, Luder HU, Leblond C-P (1991) Changes in cells' secretory organelles and extracellular matrix during endochondral ossification in the mandibular condyle of the growing rat. Am J Anat 190: 41–73
Michaels JE (1980) Alterations in the distribution of glycoproteins in epithelial cells of murine colon after injection of tunicamycin. Cell Tissue Res 210: 121–132
Pratt RM, Yamada KM, Olden K, Ohanian SH, Hascall VC (1979) Tunicamycin-induced alterations in the synthesis of sulfated proteoglycans and cell surface morphology in the chick embryo fibroblast. Exp Cell Res 118: 245–252
Roth J, Bendayan M, Orci L (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem 26: 1074–1081
Roth J, Bendayan M, Orci L (1980) FITC-protein A-gold complex for light and electron microscopic immunocytochemistry. J Histochem Cytochem 28: 55–57
Serafini-Fracassini A, Smith JW (1974) Glycosaminoglycans and proteoglycans. In: Serafini-Fracassini A, Smith JW (eds) The structure and biochemistry of cartilage. Churchill Livingstone, Edinburgh London, pp 64–112
Struck DK, Lennarz WJ (1980) The function of saccharide-lipids in synthesis of glycoproteins. In: Lennarz WJ (ed) The biochemistry of glycoproteins and proteoglycans. Plenum Press, New York London, pp 35–83
Tamaki H, Yamashina S (1987) In vivo effects of tunicamycin on secretory processes of rat parotid glands. Cell Tissue Res 250: 323–330
Velasco A, Hidalgo J, Müller M, Garcia-Herdugo G (1988) Ultrastructural demonstration of lectin binding sites in the Golgi apparatus of rat epiphyseal chondrocytes. Histochemistry 89: 177–184
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Yokose, S., Tajima, Y. In vivo effects of tunicamycin on chondrocytes of rat mandibular condyles as revealed by lectin cytochemistry. Cell Tissue Res 269, 235–239 (1992). https://doi.org/10.1007/BF00319614
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DOI: https://doi.org/10.1007/BF00319614