Skip to main content
SpringerLink
Log in

Demonstration of atrial natriuretic peptide/cardiodilatin (ANP/CDD)-immunoreactivity in the salt gland of the pekin duck

  • Published:
Anatomy and Embryology Aims and scope Submit manuscript

Summary

A novel peptide hormone, atrial natriuretic factor/cardiodilatin (ANP/CDD), was recently isolated and characterized from mammalian heart. Its presence has been demonstrated in several organs that contribute to water and sodium homeostasis, such as salivary glands. This study demonstrates the presence of ANP/CDD immunoreactivity in the salt gland of Pekin ducks by high performance liquid chromatography, radioimmunoassay and immunocytochemistry, using a specific antibody against atriopeptide I. A small number of distinct, ovoid or cuboid shaped ANP/CDD-immunoreactive cells were localized in the connective tissue surrounding and separating the central secretory tubules, whereas no immunostaining was observed in the peripheral tubules. Salt glands of ducks that were adapted to salt water revealed a significant hypertrophy of their secretory lobules. However, no differences were found between the number or localization of immunoreactive cells in the salt gland of salt water-acclimatized ducks and non-stimulated glands of ducks that were housed with ad libitum access to fresh water. Our results indicate that ANP/CDD may play a role in the regulation of sodium secretion in the salt gland of aquatic birds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ash RW, Pearce JW, Silver A (1969) An investigation of the nerve supply to the salt gland of the duck. J Exp Physiol 54:281–295

    Google Scholar 

  • Atarashi K, Mulrow RJ, Franco-Saez R, Snajdar R, Rapp J (1984) Inhibition of aldosterone production by an atrial extract. Science 224:992–994

    Google Scholar 

  • Bianchi C, Gutkowska J, Garcia R, Thibault G, Genest J, Cantin M (1987) Localization of 125I-atrial natriuretic factor (ANF)-binding sites in rat renal medulla. A light and electron microscope autoradiographic study. J Histochem Cytochem 35:149–153

    Google Scholar 

  • Burnett JC, Jr, Graujer JP, Opgennoth TS (1984) Effects of synthetic atrial natriuretic factor on renal function and renin release. Am J Physiol 247:F863-F867

    Google Scholar 

  • Burock G, Kühnel W, Petry G (1969) Über die inaktive Salzdrüse von Enten (Anas platyrhinchus). Histologische und histochemische Untersuchungen. Z Zellforsch 97:608–618

    Google Scholar 

  • Cantin M, Genest J (1985) The heart and the atrial natriuretic factor. Endocrinol Rev 6:107–127

    Google Scholar 

  • Cantin M, Gutkowska J, Thibault G, Milne RW, Ledoux S, MinLi S, Chapeau C, Garcia R, Hamet P, Genest J (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Histochemistry 80:113–127

    Google Scholar 

  • Chartier L, Schiffrin E, Thibault G, Garcia R (1984) Atrial natriuretic factor inhibits the stimulation of aldosterone secretion by angiotensin II, ACTH and potassium in vitro and angiotensin II-induced steroidogenesis in vivo. Endocrinology 115:2026–2028

    Google Scholar 

  • Currie MG, Geller DM, Cole BR, Seigel NR, Fok KF, Adams SP, Eubanks SR, Galluppi GR, Needleman P (1984) Purification and sequence analysis of bioactive atrial peptides (atriopeptins). Science 223:67–69

    Google Scholar 

  • De Bold AJ (1979) Heart atria granularity: effects of changes in water-electrolyte balance. Proc Soc Exp Biol Med 161:508–511

    Google Scholar 

  • De Bold AJ (1985) Atrial natriuretic factor: a hormone produced by the heart. Science 230:767–770

    Google Scholar 

  • De Bold AJ, Flynn TG (1983) Cardionatrin I — A novel heart peptide with potent diuretic and natriuretic properties. Life Sci 33:297–302

    Google Scholar 

  • De Bold AJ, Borenstein HB, Veress AT, Sonnenberg H (1981) A rapid and potent natriuretic response to intravenous injection of atrial mycardial extract in rats. Life Sci 28:89–94

    Google Scholar 

  • Fänge R, Robinson M (1958) Control of secretion from the avian salt gland. Am J Physiol 195:321–326

    Google Scholar 

  • Flynn TG, De Bold ML, De Bold AJ (1983) The aminoacid sequence of an atrial peptide with potent diuretic and natriuretic properties. Biochem Biophys Res Commun 117:859–865

    Google Scholar 

  • Forssmann WG (1986) Cardiac hormones. I. review on the morphology, biochemistry and molecular biology of the endocrine heart. Eur J Clin Invest 16:439–451

    Google Scholar 

  • Forssmann WG, Hock D, Lottspeich F, Henschen A, Kreye V, Christmann M, Reinecke M, Metz J, Carlquist M, Mutt V (1983) The right auricle of the heart is an endocrine organ. Cardiodilatin as a peptide hormone candidate. Anat Embryol 168:307–313

    Google Scholar 

  • Forssmann WG, Birr C, Carlquist M, Christmann M, Finke R, Henschen A, Hock D, Kirchheim H, Kreye V, Lottspeich F, Metz J, Mutt V, Reinecke M (1984) The auricular myocardiocytes of the heart constitute an endocrine organ. Characterization of a porcine cardiac peptide hormone, cardiodilatin-126. Cell Tissue Res 238:425–430

    Google Scholar 

  • Gerstberger R (1988) Functional vasoactive intestinal polypeptide (VIP)-system in salt glands of the Pekin duck. Cell Tissue Res 252:39–48

    Google Scholar 

  • Gerstberger R, Simon-Oppermann C, Kaul R (1984) Cephalic osmoreceptor control of salt gland activation and inhibition in the salt adapted duck. J Comp Physiol 154:449–456

    Google Scholar 

  • Grammer RT, Fukumi H, Inagami T, Misono KS (1983) Rat atrial natriuretic factor. Purification and vasorelaxant activity. Biochem Biophys Res Commun 116:696–708

    Google Scholar 

  • Herbst M, Goebel J, Born AJ, Metz J (1986) Immunhistochemische Untersuchungen der Gangepithelien der menschlichen Parotis. Abstr 81. Vers Anat Ges, Lubeck, p 71

  • Hootman SR, Ernst SA (1982) 3H-QNB binding to muscarinic receptors in intact avian salt gland cells. Am J Physiol 243:C254-C261

    Google Scholar 

  • Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence of alpha-human atrial natriuretic polypeptide (alpha-h ANP). Biochem Biophys Res Commun 118:131–139

    Google Scholar 

  • Komnick H (1963a) Elektronenmikroskopische Untersuchungen zur funktionellen Morphologie des Ionentransports in der Salzdrüse von Larus argentatus. I. Teil: Bau und Feinstruktur der Salzdrüse. Protoplasma 56:274–314

    Google Scholar 

  • Komnick H (1963b) Elektronenmikroskopische Untersuchungen zur funktionellen Morphologie des Ionentransporter in der Salzdrüse von Larus argentatus. II. Teil: Funktionelle Morphologie der Tubulusepithelzellen. Protoplasma 56:605–636

    Google Scholar 

  • Komnick H (1964) Elektronenmikroskopische Untersuchungen zur funktionellen Morphologie des Ionentransportes in der Salzdrüse von Larus argentatus. IV. Teil: Funktionelle Morphologic der Epithelzellen des Sammelkanals. Protoplasma 56:605–636

    Google Scholar 

  • Kühnel W, Burock G, Petry G (1969a) Enzymhistotopochemische Studien an inaktiven Salzdrüsen von Hausenten (Anas plytyrhinchus). I. Histiogramme einiger Oxydoreduktasen. Histochemie 19:235–247

    Google Scholar 

  • Kühnel W, Petry G, Burock G (1969b) Enzymhistochemische Studien an inaktiven Salzdrüsen von Hausenten (Anas platyrhinchus). II. Cytochemische Lokalisation einiger Hydrolasen. Z Zellforsch 99:560–569

    Google Scholar 

  • Kühnel W (1972) On the innervation of the salt gland. Z Zellforsch 134:435–438

    Google Scholar 

  • Krejs GJ (1982) Effect of VIP infusion on water and electrolyte transport in the human intestine. In: Said SI (ed) Vasoactive intestinal polypeptide. Advances in Peptide Hormone Research Series. Raven Press, New York, pp 193–200

    Google Scholar 

  • Lang RE, Tholken H, Ganten D, Luft FC, Rushkoaho H, Unger Th (1985) Atrial natriuretic factor — a circulating hormone stimulated by volume loading. Nature 314:264–266

    Google Scholar 

  • Marin-Grez M, Fleming JT, Steinhausen M (1986) Atrial natriuretic peptide causes pre-glomerular vasodilatation and post-glomerular vasoconstriction in rat kidney. Nature 324:473–476

    Google Scholar 

  • Metz J, Mutt V, Forssmann WG (1984) Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria. Anat Embryol 170:123–127

    Google Scholar 

  • Nemer M, Lavigne J-P, Drouin J, Thibault G, Gannon M, Antakly T (1986) Expression of atrial natriuretic factor gene in heart ventricular tissue. Peptides 7:1147–1152

    Google Scholar 

  • Oshima T, Currie M, Geller AM, Needleman P (1984) An atrial peptide is a potent renal vasodilator substance. Circ Res 54:612–616

    Google Scholar 

  • Schmidt-Nielsen K (1959) Salt glands. Sci Am 200:109–116

    Google Scholar 

  • Schmidt-Nielsen K (1960) The salt secreting gland of marine birds. Circulation 21:955–967

    Google Scholar 

  • Sofroniew MV (1983) Golgi-like immunoperoxidase staining of neurons producing specific substances or of neurons transporting exogenous tracer proteins. In: Cuello AC (ed) Immunohistochemistry, Chapter 16, IBRO, Raven Press, New York, pp 431–447

    Google Scholar 

  • Standaert DG, Saper CB, Needleman Ph (1985) Atriopeptin: a potent hormone and potential neuromediator. TINS 8:509–511

    Google Scholar 

  • Sternberger L (1979) Immunocytochemistry, 2nd edn, John Wiley & Sons, New York

    Google Scholar 

  • Wood WG, Ballentyne B (1968) Sodium ion transport and betaglucuronidase activity in the nasal gland of Anas domesticus. J Anat (Lond) 103:277–287

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, University of Munich, Pettenkoferstrasse 11, D-8000, München 2, Federal Republic of Germany

    W. Lange & J. Unger

  2. Department of Neurology, Technical University of Munich, Pettenkoferstrasse 11, D-8000, München 2, Federal Republic of Germany

    A. Weindl

  3. Department of Pharmacology, University of Heidelberg, Im Neuenheimer Feld 366, D-6900, Heidelberg, Federal Republic of Germany

    R. E. Lang

Authors
  1. W. Lange
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Unger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Weindl
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. E. Lang
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Dedicated to Prof. Dr. med. Dr. phil. h.c. D. Starck on the occasion of his 80th birthday

Supported by the Walter-Schulz-Stiftung and Deutsche Forschungsgemeinschaft DFG, We 608/8-2

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lange, W., Unger, J., Weindl, A. et al. Demonstration of atrial natriuretic peptide/cardiodilatin (ANP/CDD)-immunoreactivity in the salt gland of the pekin duck. Anat Embryol 179, 465–469 (1989). https://doi.org/10.1007/BF00319589

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00319589

Key words

Search

Navigation