Summary
Degenerating prespermatogonial germ cells in the testis of the immature golden hamster [aged 14 days post conceptionem (dpc) to 13 days post partum [dpp)] were studied with regard to their morphology and temporal incidence. Judged by their ultrastructural features, these cells clearly take the form of apoptosis and finally are subjected to phagocytosis by neighboring Sertoli cells; only a few germ cells of a presumably incipient, partly variant degenerative morphology cannot, at present, be assigned to the apoptotic mode of cellular death. Degenerating prespermatogonia occur between the 14th dpc and 3rd dpp and again, after an interval in which no such cells are found, from the 9th dpp onwards. This pattern reveals a striking parallelism to the phases of proliferation of these cells, viz., the appearance of M- and T2-prespermatogonia. Both this obvious temporal association of proliferation and degeneration and the classification of prespermatogonial death as apoptosis suggest some developmental significance of the degenerative phenomena investigated.
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References
Allan DJ, Harmon BV, Kerr JFR (1987) Cell death in spermatogenesis. In: Potten CS (ed) Perspectives on mammalian cell death. Oxford University Press, Oxford New York Tokyo, pp 229–258
Allen E (1949) Studies on degenerating sex cells in immature mammals. I. An analysis of degeneration in primordial and large germ cells in male albino rats aged 1–9 days. J Morphol 85:405–421
Beaulaton J, Lockshin RA (1982) The relation of programmed cell death to development and reproduction: comparative studies and an attempt at classification. Int Rev Cytol 79:215–235
Clarke PGH (1990) Developmental cell death: morphological diversity and multiple mechanisms. Anat Embryol 181:195–213
Clermont Y (1962) Quantitative analysis of spermatogenesis of the rat: a revised model for the renewal of spermatogonia. Am J Anat 111:111–129
Cohen JJ, Duke RC (1984) Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death. J Immunol 132:38–42
Duvall E, Wyllie AH, Morris RG (1985) Macrophage recognition of cells undergoing programmed cell death (apoptosis). Immunology 56:351–358
Fawcett DW (1975) Ultrastructure and function of the Sertoli cell. In: Hamilton DW, Greep RO (eds) Handbook of physiology, sect 7, vol 5: Male reproductive system. American Physiological Society, Washington DC, pp 21–55
Glücksmann A (1951) Cell deaths in normal vertebrate ontogeny. Biol Rev 26:59–86
Gondos B, Byskov AG (1981) Germ cell kinetics in the neonatal rabbit testis. Cell Tissue Res 215:143–151
Gondos B, Hobel CJ (1973) Germ cell degeneration and phagocytosis in the human foetal ovary. In: Peters H (ed) The development and maturation of the ovary and its functions. Excerpta Medica, Amsterdam, pp 77–83
Gondos B, Zamboni L (1969) Ovarian development: the functional importance of germ cell interconnections. Fertil Steril 20:176–189
Hilscher B, Hilscher W, Bülthoff-Ohnolz B, Krämer U, Birke A, Pelzer H, Gauss G (1974) Kinetics of gametogenesis. I. Comparative histological and autoradiographic studies of oocytes and transitional prospermatogonia during oogenesis and prespermatogenesis. Cell Tissue Res 154:443–470
Hilscher W, Makoski H-B (1968) Histologische und autoradiographische Untersuchungen zur “Präspermatogenese” und “Spermatogenese” der Ratte. Z Zellforsch 86:327–350
Huckins C (1978) The morphology and kinetics of spermatogonial degeneration in normal adult rats: an analysis using a simplified classification of the germinal epithelium. Anat Rec 190:905–926
Huckins C, Clermont Y (1968) Evolution of gonocytes in the rat testis during late embryonic and early post-natal life. Arch Anat Histol Embryol 51:341–354
Johannisson E (1968) The foetal adrenal cortex in the human. Its ultrastructure at different stages of development and in different functional states. Acta Endocrinol (Copenhagen) 58 [Suppl 130]:7–107
Kerr JFR, Wyllie AH, Currie AR (1972) Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26:239–257
Kerr JFR, Bishop CJ, Searle J (1984) Apoptosis. Rec Adv Histopathol 12:1–15
Kerr JFR, Searle J, Harmon BV, Bishop CJ (1987) Apoptosis. In: Potten CS (ed) Perspectives on mammalian cell death, Oxford University Press, Oxford New York Tokyo, pp 93–128
Larramendi PCH (1985) Method of retrieval of one to two micron sections from glass for ultrathin sectioning. J Electron Microsc Tech 2:645–646
Miething A (1990) Intercellular bridges between germ cells in the immature golden hamster testis: evidence for clonal and nonclonal mode of proliferation. Cell Tissue Res 262:559–567
Nussdorfer GG (1970) The fine structure of the newborn rat adrenal cortex. II. Zona juxtamedullaris. Z Zellforsch 103:398–409
Oakberg EF (1956) A description of spermiogenesis in the mouse and its use in analysis of the cycle of the seminiferous epithelium and germ cell renewal. Am J Anat 99:391–413
Pineau C, Le Magueresse B, Courtens J-L, Jégou B (1991) Study in vitro of the phagocytic function of Sertoli cells in the rat. Cell Tissue Res 264:589–598
Roosen-Runge EC (1955) Untersuchungen über die Degeneration samenbildender Zellen in der normalen Spermatogenese der Ratte. Z Zellforsch 41:221–235
Roosen-Runge EC (1964) The degeneration of pre-spermatogonial germ cells in the rat after birth. Anat Rec 148:328
Roosen-Runge EC (1973) Germinal-cell loss in normal metazoan spermatogenesis. J Reprod Fertil 35:339–348
Roosen-Runge EC, Leik J (1968) Gonocyte degeneration in the postnatal male rat. Am J Anat 122:275–300
Russell LD, Clermont Y (1977) Degeneration of germ cells in normal, hypophysectomized and hormone treated hypophysectomized rats. Anat Rec 187:347–366
Saunders JW (1966) Death in embryonic systems. Science 154:604–612
Schabtach E, Parkening TA (1974) A method for sequential high resolution light and electron microscopy of selected areas of the same material. J Cell Biol 61:261–264
Schulze C (1974) On the morphology of the human Sertoli cell. Cell Tissue Res 153:339–355
Searle J, Kerr JFR, Bishop CJ (1982) Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance. Pathol Annu 17:229–259
Trump BF, Berezesky IK, Osornio-Vargas AR (1981) Cell death and the disease process. The role of calcium. In: Bowen ID, Lockshin RA (eds) Cell death in biology and pathology, Chapman and Hall, London New York, pp 209–242
Wartenberg H (1974) Spermatogenese — Oogenese: ein cyto-morphologischer Vergleich. Verh Anat Ges 68:63–92
Wartenberg H (1976) Comparative cytomorphologic aspects of the male germ cells, especially of the “gonia”. Andrologia 8:117–130
Wyllie AH (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature 284:555–556
Wyllie AH (1981) Cell death: a new classification separating apoptosis from necrosis. In: Bowen ID, Lockshin RA (eds) Cell death in biology and pathology, Chapman and Hall, London New York, pp 9–34
Wyllie AH, Kerr JFR, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 68:251–306
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Miething, A. Germ-cell death during prespermatogenesis in the testis of the golden hamster. Cell Tissue Res 267, 583–590 (1992). https://doi.org/10.1007/BF00319381
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DOI: https://doi.org/10.1007/BF00319381