Summary
Follicular ruptures with intra-ovarian oocyte release (IOR) were studied in 17, 21 and 24-day-old rats by morphological methods. Using a light microscope, it was seen that IOR occurred at all times and the IOR frequency did not change. IOR developed in preantral follicles. Their oocytes were mostly found within the follicular compartment (incomplete IOR). Using an electron microscope, a circumscribed dissolution of the basal lamina was observed. IOR granulosa cells appeared activated. They rarely underwent typical necrosis after herniation into the extrafollicular area. Herniated granulosa cells tended either to stay intact or to shed cytoplasmic components into the extracellular space. whilst nuclei of active cell function were maintained. Tissue adjacent to an IOR seemed inactive with the exception of endothelial cells. Some endothelial cells underwent necrosis. Additionally, the endothelium was discontinous. The morphological data support the hypothesis that the mechanism of follicular rupture represents an inside to outside process.
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References
Andersen AC, Simpson ME (1973) The ovary and the reproductive cycle of the dog (Beagle). Geron-X, Los Altos, Calif
Beers WH, Strickland S, Reich E (1975) Ovarian plasminogen activator: relationship to ovulation and hormonal regulation. Cell 6:387–394
Bjersing L, Cajander S (1974a) Ovulation and the mechanism of follicle rupture. IV. Ultrastructure of membrana granulosa of rabbit Graafian follicles prior to induced ovulation. Cell Tissue Res 153:1–14
Bjersing L, Cajander S (1974b) Ovulation and the mechanism of follicle rupture. V. Ultrastructure of tunica albuginea and theca externa of rabbit Graafian follicles prior to induced ovulation. Cell Tissue Res 153:15–30
Bjersing L, Cajander S (1974c) Ovulation and the mechanism of follicle rupture. VI. Ultrastructure of theca interna and the inner vascular network surrounding rabbit Graafian follicles prior to induced ovulation. Cell Tissue Res 153:31–44
Burghardt RC, Matheson RL (1982) Gap junction amplification in rat ovarian granulosa cells. I. A direct response to follicle-stimulating hormone. Dev Biol 94:206–215
Byskov AG (1969) Ultrastructural studies on the preovulatory follicle in the mouse ovary. Z Zellforsch 100:285–299
Byskov AG, Rasmussen G (1973) Ultrastructural studies of the developing follicle. In: Peters H (ed) The development and maturation of the ovary and its function. Excerpta Medica, Amsterdam, pp 55–62
Cajander, S, Bjersing L (1976) Further studies of the surface epithelium covering preovulatory rabbit follicles with special reference to lysosomal alterations. Cell Tissue Res 169:129–141
Dawson AB, McCabe M (1951) The interstitial tissue of the ovary of infantile and juvenile rats. J Morphol 88:543–564
Downs SM, Longo FJ (1982) Effects of indomethacin on preovulatory follicles in immature superovulated mice. Am J Anat 164:265–274
Downs SM, Longo FJ (1983) An ultrastructural study of prevulatory apical development in mouse ovarian follicles: effects of indomethacin. Anat Rec 205:159–168
Espey LL (1980) Ovulation as an inflammatory action — a hypothesis. Biol Reprod 22:73–106
Ito S, Karnovsky MJ (1968) Formaldehyde-glutaraldehyde fixatives containing trinitro compounds. J Cell Biol 39:168
Karnovsky MJ (1971) Use of ferrocynide-reduced osmium tetroxide in electron microscopy. J Cell Biol 51:146A
Larsen WJ (1977) Structural diversity of gap junctions. A review. Tissue Cell 9:373–394
McNutt N, Weinstein RS (1973) Membrane ultrastructure at mammalian intercellular junctions. Progr Biophys Mol Biol 26:45–102
Merk FB, Albright JT, Botticelli CR (1973) The fine structure of granulosa cell nexuses in rat ovarian follicles. Anat Rec 175:107–125
Okuda J, Okamura H, Kanzaki H, Fuji S, Takenaka A, Wallach EE (1983) An ultrastructural study of ovarian perifollicular capillaries in the indomethacin-treated rabbit. Fertil Steril 39:85–92
Rawson, JMR, Espey LL (1977) Concentration of electron dense granules in the rabbit ovarian surface epithelium during ovulation. Biol Reprod 17:561–566
Richardson KC, Jarrett L, Finke EH (1960) Embedding in epoxy resins for ultrathin sectioning in electron microscopy. Stain Technol 35:313–323
Spanel-Borowski K, Petterborg LJ, Reiter RJ (1982a) Preantral intra-ovarian oocyte release in the white-footed mouse, Peromyscus leucopus. Cell Tissue Res 226:461–464
Spanel-Borowski K, Vaughan MK, Johnson LY, Reiter RJ (1982b) Occurrence of preantral intra-ovarian oocyte release in the rat and the Syrian hamster (Mesocricetus auratus). Anat Embryol 165:169–175
Spanel-Borowski K, Vaughan MK, Johnson LY, Reiter RJ (1983) Increase of intra-ovarian oocyte release in PMSG-primed immature rats and its inhibition by arginine vasotocin. Biomed Res 4:71–82
Walter JB, Israel MS (1979) Some disorders of the blood: the platelets. In: General pathology, 8th edn. Churchill Livingstone, London New York, pp 633–644
Zamboni L (1970) Ultrastructure of mammalian oocytes andova. Biol Reprod [Suppl] 2:44–63
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Spanel-Borowski, K., Aumüller, G. Light and ultrastructure of intra-ovarian oocyte release in infantile rats. Anat Embryol 172, 331–337 (1985). https://doi.org/10.1007/BF00318981
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DOI: https://doi.org/10.1007/BF00318981