Summary
-
1.
Embryonic tissues (ectoderm, neural plate) of Ambystoma mexicanum and Triturus were killed with hot water and implanted into ectoderm sandwiches. They induced the ectoderm to form neural tissue, lentoids and unspecialized epidermis. Neural tissue always showed retina character. Egg pigment was eliminated and gathered at the outer retinal surface or in the centre of rosettes. Neural crest cells like mesenchyme or melanophores were completely lacking, retinal pigment did not develop.
-
2.
The thus induced living retina tissue was reimplanted into fresh ectoderm after 2 days. It continued histogenetic and morphogenetic differentiation and formed ocular vesicles with numerous eye cups. It induced the enveloping ectoderm to again form retina, lentoids and unspecialized epidermis without neural crest derivatives or RPE.
-
3.
This inductive process can be reproduced several times.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barth, L.G.: Neural differentiation without organizer. J. exp. Zool. 87, 371–383 (1941)
Chuang, H.: Induktionsleistungen von frischen und gekochten Organteilen (Niere, Leber) nach ihrer Verpflanzung in Explantate und verschiedene Wirtsregionen von Tritonkeimen. W. Roux' Arch. 139, 556–638 (1939)
Fujisawa, H.: The process of reconstruction of histological architecture from dissociated retina cells. W. Roux' Arch. 171, 312–330 (1973)
Harrison, R.G.: Organization and Development of the Embryo. New Haven and London: Yale Univ. Press 1969
Holtfreter, J.: Über die Aufzucht isolierter Teile des Amphibienkeimes. W. Roux' Arch. 124, 422–510 (1931)
Holtfreter, J.: Der Einfluß von Wirtsalter und verschiedenen Organbezirken auf die Differenzierung von angelagertem Gastrulaektoderm. W. Roux' Arch. 127, 619–775 (1933)
Holtfreter, J.: Neural differentiation of ectoderm through exposure to saline solution. J. exp. Zool. 95, 307–340 (1944b)
Holtfreter, J.: Neural induction in explants which have passed through a sublethal cytolysis. J. exp. Zool. 106, 197–222, (1947c)
Holtfreter, J.: Studies on the diffusibility, toxity and pathogenic properties of “inductive” agents derived from dead tissues. Exp. Cell Res. (suppl.) 3, 188–209 (1955)
Hori, R., Nieuwkoop, P.D.: Induction phenomena with denaturated inductor. Ned. Akad. Wet., Proc. Ser. C, Biol. and Med. Sc. 58, 266–272 (1955)
ter Horst, J.: Differenzierungs- und Induktionsleistungen verschiedener Abschnitte der Medullarplatte und des Urdarmdaches von Triton im Kombinat. W. Roux' Arch. 143, 275–303 (1948)
Ikeda, Y.: Über die wechselseitigen Beziehungen der Sinnesorgane untereinander in ihrer normalen und experimentell bedingten Entwicklung. Arb. anat. Inst. Sendai 21, (1938)
Mangold, O.: Über die Induktionsfähigkeit der verschiedenen Bezirke der Neurula von Urodelen. Naturwissenschaften 21, 761–766 (1933b)
Nieuwkoop, P.D.: Pattern formation in artificially activated ectoderm (Rana pipiens and Ambystoma punctatum). Develop. Biol. 7, 255–279 (1963)
Rollhäuser-ter Horst, J.: Die Induktionsleistung verschiedener Tritonkeimteile nach Denaturierung. W. Roux' Arch. 146, 183–200 (1953)
Sala, M.: The character of the “local activations” formed in sandwich explants of competent ectoderm containing neural formations induced by different cranio-caudal portions of the archenteron roof. Proc. kon. ned. Akad. Wet. 59, 662–667 (1956)
Saxen, L., Toivonen, S.: Primary Embryonic Induction. London: Academic Press Inc. 1962
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rollhäuser-ter Horst, J. Artificial neural induction in amphibia. Anat Embryol 151, 309–316 (1977). https://doi.org/10.1007/BF00318932
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00318932