Summary
Cationic ferritin was used as a marker to reveal the processes of endocytosis and intracellular transport in bronchiolar and alveolar epithelia. The marker was injected into the lung via the trachea, and ultrastructural observation of the distribution of ferritin particles in bronchiolar and alveolar epithelial cells was carried out at intervals of 5, 15, 30 and 60 min after the injection. The luminal surface of the airway and the alveolar epithelium showed diffuse labeling with cationic ferritin. In general, ferritin particles were observed in vesicles and vacuoles of the bronchiolar and alveolar epithelial cells within 5 min of injection; they appeared in multivesicular bodies within 15 min. Multivesicular bodies and secondary lysosomes containing ferritin particles, some of which showed a positive reaction for acid phosphatase, were seen in the basal cytoplasm within 30 min; ferritin particles appeared in the basal lamina below the Clara cells, ciliated cells and type 2 alveolar cells within 30 min. Ferritin particles were seen in ovoid granules of some Clara cells and in lamellar inclusion bodies of many type 2 alveolar cells. Brush cells and type 1 alveolar cells took up only a small quantity of ferritin particles.
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References
Bignon J, Chahinian P, Feldmann G, Sapin C (1975) Ultrastructural immunoperoxidase demonstration of autologous albumin in the alveolar capillary membrane and in the alveolar lining material in normal rats. J Cell Biol 64:503–509
Crandall ED, Kim KJ (1989) Protein traffic across lung epithelia. Am J Respir Cell Mol Biol 1:255
DeFouw DO (1983) Ultrastructural features of alveolar epithelial transport. Am Rev Respir Dis 127:S9-S13
Farquhar MG (1978) Recovery of surface membrane in anterior pituitary cells. Variation in traffic detected with anionic and cationic ferritin. J Cell Biol 77:R35-R42
Farquhar MG (1983) Multiple pathways of exocytosis, endocytosis, and membrane recycling: validation of a Golgi route. Fed Proc 42:2407–2413
Gail DB, Lenfant CJM (1983) Cells of the lung: biology and clinical implications. Am Rev Respir Dis 127:366–387
Gil J (1983) Number and distribution of plasmalemmal vesicles in the lung. Fed Proc 42:2414–2418
Gomori G (1952) Microscopic histochemistry; principle and practice. University Chicago Press, Chicago
Goniakowska-Witalińska L, Lauweryns JM (1991) Brush cells in the lungs of Bombina orientalis (Boul.) (Anura, Amphibia). J Submicrosc Cytol Pathol 23:123–130
Iseki S, Kondo H (1990) An immunocytochemical study on the occurrence of liver fatty-acid-binding protein in the digestive organs of rat: specific localization in the D cells and brush cells. Acta Anat 138:15–23
Iseki S, Kanda T, Hitomi M, Ono T (1991) Ontogenic appearance of three fatty acid proteins in the rat stomach. Anat Rec 229:51–60
Jeffery PK (1987) Structure and function of adult tracheobronchial epithelium. In: McDowell EM (ed) Lung carcinomas, Churchill Livingstone, Edinburgh London Melbourne, pp 42–73
Kalina M, Socher R (1990) Internalization of pulmonary surfactant into lamellar bodies of cultured rat pulmonary type II cells. J Histochem Cytochem 38:483–492
Kuhn C (1978) Ultrastructure and cellular function in the distal lung. In: Thurlbeck MW, Abell MR (eds) The lung. Structure, function and disease. Williams and Wilkins, Baltimore, pp 1–20
Kuhn C, Callaway LA (1975) The formation of granules in the bronchiolar Clara cells of the rat. II. Enzyme histochemistry. J Ultrastruct Res 53:66–76
Lauweryns JM, Baert JH (1974) The role of the pulmonary lymphatics in the defenses of the distal lung: morphological and experimental studies of the transport mechanisms of intratracheally instilled particles. Ann NY Acad Sci 221:244–275
Matlin KS, Reggio H, Helenius A, Simons K (1981) Infectious entry pathway of influenza virus in a canine kidney cell line. J Cell Biol 91:601–603
Mossman BT, Eastman A (1987) Carcinogenesis in the respiratory tract. In: McDowell EM (ed) Lung carcinomas. Churchill Livingstone, Edinburgh London Melbourne, pp 129–161
Mostov KE, simister NE (1985) Transcytosis. Cell 43:389–390
Petrik P, Collet AJ (1974) Quantitative electron microscopic autoradiography of in vivo incorporation of 3H-choline, 3H-leucine, 3H-acetate and 3H-galactose in non-ciliated bronchiolar (Clara) cells of mice. Am J Anat 139:519–534
Ranga V, Kleinerman J (1982) The effect of pilocarpine on vesicular uptake and transport of horseradish peroxidase by the guinea pig tracheal epithelium. Am Rev Respir Dis 125:579–585
Rhodin J, Dalhamn T (1956) Electron microscopy of the tracheal ciliated mucosa in rat. Z Zellforsch 44:345–412
Richardson J, Bouchard T, Ferguson CC (1976) Uptake and transport of exogenous proteins by respiratory epithelium. Lab Invest 35:307–314
Schneeberger EE (1990) Freeze fracture in lung research. In: Gil J (ed) Models of lung disease. Microscopy and structural methods. Dekker, New York Basel, pp 57–71
Schneeberger EE, Karnovsky MJ (1971) The influence of intravascular fluid volumes on the permeability of newborn and adult mouse lung to ultrastructural protein tracer. J Cell Biol 49:319–334
Silverstein SC, Steinman RM, Cohn ZA (1977) Endocytosis. Annu Rev Biochem 46:669–722
Simionescu M, Ghinea N (1990) The use of tracers of transport studies. In: Gil J (ed) Models of lung disease. Microscopy and structural methods. Dekker, New York Basel, pp 359–408
Spicer SS, Schulte BA, Thomopoulos GN (1983) Histochemical properties of the respiratory tract epithelium in different species. Am Rev Respir Dis 128:S20-S26
Trier JS, Allan CH, Marcial MA, Madara JL (1987) Structural features of the apical and tubulovesicular membranes of rodent small intestinal tuft cells. Anat Rec 219:69–77
Williams MC (1984a) Endocytosis in alveolar type II cells: effect of charge and size of tracers. Proc Natl Acad Sci USA 81:6054–6058
Williams MC (1984b) Uptake of lectins by pulmonary alveolar type II cells: subsequent deposition into lamellar bodies. Proc Natl Acad Sci USA 81:6383–6387
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Ito, T., Kitamura, H., Inayama, Y. et al. Uptake and intracellular transport of cationic ferritin in the bronchiolar and alveolar epithelia of the rat. Cell Tissue Res 268, 335–340 (1992). https://doi.org/10.1007/BF00318802
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DOI: https://doi.org/10.1007/BF00318802