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Immunocytological localization of 1,25-dihydroxyvitamin D3-like molecules and their receptors in a calcium-transporting epithelium of a crustacean

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Summary

For the first time, immunoreactivity to 1,25(OH)2D3-like molecules and their receptors has been investigated in a calcium transporting epithelium of a crustacean, Orchestia, using vertebrate antisera on ultrathin cryosections of posterior caeca previously fixed in glutaraldehyde, then postfixed in osmium tetroxide. Both immunoreactivities were located mainly in the nuclei of epithelial cells. Quantitative differences in 1,25(OH)2D3-like immunoreactivity were noticed from one stage of the molt cycle to another. These results, together with other data, contribute to evidence that immunoreactive 1,25(OH)2D3-like molecules may be involved in the regulatory processes of calcium metabolism in this terrestrial crustacean and suggest an involvement of these substances in the regulation of calcium movements in the posterior caeca.

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References

  • Bailly du Bois M, Milet C, Garabedian M, Guillozo H, Martelly E, Lopez E, Balsan S (1988) Calcium-dependent metabolism of 25-hydroxycholecalciferol in silver eel tissues. Gen Comp Endocrinol 71: 1–29

    Google Scholar 

  • Boivin G, Mesguich P, Pike JW, Bouillon R, Meunier PJ, Haussler MR, Dubois PM, Morel G (1987) Ultrastructural immunocytochemical localization of endogenous 1,25-dihydroxyvitamin D3 and its receptors in osteoblasts and osteocytes from neonatal mouse and rat calvaria. Bone Mineral 3: 125–136

    Google Scholar 

  • Bouillon R, De Moor P, Baggiolini EG, Uskokovic MR (1980) A radioimmunoassay for 1,25-dihydroxy-cholecalciferol. Clin Chem 26: 562–567

    Google Scholar 

  • Clemens TL, Garrett KP, Zhou XY, Pike JW, Haussler MR, Dempster DW (1988) Immunocytochemical localization of the 1,25-dihydroxyvitamin D3 receptor in target cells. Endocrinology 122: 1224–1230

    Google Scholar 

  • De Luca HF, Schnoes HK (1983) Vitamin D: recent advances. Ann Rev Biochem 52: 411–439

    Google Scholar 

  • Edelstein S, Fine N, Shine S, Weiner S (1985) Is vitamin D a calcium regulating hormone in invertebrates? In: Norman AW, Schaefer R, Grigoleit HG, Herrath DV (eds) Vitamin D, a chemical, biochemical and clinical update. de Gruyter Berlin, pp 717–722

    Google Scholar 

  • Garabedian M (1984) Les activités biologiques de la vitamine D. In: Fournier A, Garabedian M, Sebert JL (eds) Vitamine D et maladies des os et du métabolisme minéral. Masson, Paris, pp 21–39

    Google Scholar 

  • Graf F (1969) Le stockage de calcium avant la mue chez les Crustacés Amphipodes Orchestia (Talitridé) et Niphargus (Gammaridé hypogé). Thèse Doct Sci Nat Dijon No105 Imp Berthier, Arch Orig Centre Document CNRS no2690, pp 1–216

  • Graf F (1986) Fine determination of the molt cycle stages in Orchestia. J Crustacean Biol 6: 666–678

    Google Scholar 

  • Graf F, Meyran JC (1983) Premolt calcium secretion in midgut posterior caeca of the crustacean Orchestia: ultrastructure of the epithelium. J Morphol 177: 1–23

    Google Scholar 

  • Graf F, Meyran JC (1985) Postexuvial calcium reabsorption in midgut posterior caeca of the crustacean Orchestia cavimana: ultrastructural changes of the epithelium. Cell Tissue Res 242: 83–95

    Google Scholar 

  • Graf F, Michaut P (1977) Les sphérules calciques de l'épithélium caecal d'Orchestia (Crustacé, Amphipode), forme de transport de calcium dans le sens apico-basal. CR Acad Sci 284: 49–52

    Google Scholar 

  • Graf F, Michaut P (1980) Fine structure of the migut posterior caeca in the crustacean Orchestia in intermolt: recognition of two distinct segments. J Morphol 165: 261–284

    Google Scholar 

  • Graf F, Fouchereau-Peron M, Van-Wormhoudt A, Meyran JC (1989) Variations of calcitonin-like immunoreactivity in the Crustacean Orchestia cavimana during a molt cycle. Gen Comp Endocrinol 73: 80–84

    Google Scholar 

  • Haussler MR, Donaldson CA, Kelly MA, Mangelsdorf DJ, Marion SL, Pike JW (1985) Functions and mechanism of action of the 1,25-dihydroxyvitamin D3 receptor. In: Norman AW, Schaefer R, Grigoleit HG, Herrath DV (eds) Vitamin D, a chemical, biochemical and clinical update. de Gruyter, Berlin, pp 83–92

    Google Scholar 

  • Haussler MR, Mangelsdorf DJ, Komm BS, Terpening CM, Yamaoka K, Allegretto EA, Baker AR, Shine J, McDonnel DP, Hughes M, Weigel NL, O'Malley BW, Pikes JW (1988) Molecular biology of the vitamin D hormone. Rec Prog Horm Res 44: 263–305

    Google Scholar 

  • Haussler MR, Mc Cain TA (1977) Vitamin D metabolism and action. N Engl J Med 297: 974–981

    Google Scholar 

  • Henry HL, Norman AW (1984) Vitamin D: metabolism and biological actions. Ann Rev Nutr 4: 493–520

    Google Scholar 

  • Hobbs RN, Pennock JF (1977) A new metabolite of vitamin D in the small sea-urchin Psammechinus miliaris. Biochem Soc Trans 5: 1713–1714

    Google Scholar 

  • Kumar R (1986) The metabolism and mechanisms of action of 1,25-dihydroxyvitamin D3. Kidney Int 30: 793–820

    Google Scholar 

  • Lehtovaara I, Koskinen T (1986) Demonstration of vitamin D3 metabolism in Mytilus edulis. Experientia 42: 147–148

    Google Scholar 

  • Marcocci C, Freake HC, Iwasaki J, Lopez E, MacIntyre I (1982) Demonstration of organ distribution of the 1,25-dihydroxyvitamin D3-binding protein in fish (A. anguilla). Endocrinology 110: 1347–1354

    Google Scholar 

  • Meyran JC (1987) Dynamique calcique transépithéliale dans les caecums postérieurs d'Orchestia cavimana Heller (Crustacé, Amphipode) au cours de la mue. Thèse Doct Sc Dijon, 1–52

  • Meyran JC, Graf F, Nicaise G (1984) Calcium pathway through a mineralizing epithelium in the crustacean Orchestia in premolt: ultrastructural cytochemistry and X-ray microanalysis. Tissue Cell 16: 269–286

    Google Scholar 

  • Meyran JC, Graf F, Nicaise G (1986) Pulse discharge of calcium through a demineralizing epithelium in the crustacean Orchestia: ultrastructural cytochemistry and X-ray microanalysis. Tissue Cell 18: 267–283

    Google Scholar 

  • Meyran JC, Chapuy MC, Arnaud S, Sellem E, Graf F (1990) Variations of vitamin D-like reactivity in the crustacean Orchestia cavimana during the molt cycle. Gen Comp Endocrinol (in press)

  • Morel G, Boivin G, David L, Dubois PM, Meunier PJ (1985) Immunocytochemical evidence for endogenous calcitonin and parathyroid hormone in osteoblasts. Absence of endogenous oestradiol and oestradiol receptors. Cell Tissue Res 240: 89–93

    Google Scholar 

  • Norman AW, Roth J, Orci L (1982) The vitamin D endocrine system: steroid metabolism, hormone receptors and biological response (calcium binding proteins). Endocr Rev 3: 331–366

    Google Scholar 

  • Pansu D, Bellaton C, Roche C (1984) Absorption intestinale du calcium et sa régulation. Phénomènes tissulaires, membranaires et moléculaires. Diabète Métab 10: 106–120

    Google Scholar 

  • Pike JW (1984) Monoclonal antibodies to chick intestinal receptors for 1,25-dihydroxyvitamin D3. J Biol Chem 259: 1167–1173

    Google Scholar 

  • Pike JW (1985) Intracellular receptors mediate the biologic action of 1,25-dihydroxyvitamin D3. Nutrition Rev 43: 161–168

    Google Scholar 

  • Pike JW, Donaldson CA, Marion SL, Haussler MR (1982) Development of hybridomas secreting monoclonal antibodies to the chicken intestinal 1α,25-dihydroxyvitamin D3 receptor. Proc Natl Acad Sci USA 43: 161–168

    Google Scholar 

  • Pike JW, Marion SL, Donaldson CA, Haussler MR (1983) Serum and monoclonal antibodies against the chick intestinal receptor for 1,25-dihydroxyvitamin D3. J Biol Chem 258: 1289–1296

    Google Scholar 

  • Sellem E, Graf F, Meyran JC (1989) Some effects of salmon calcitonin on calcium metabolism in the crustacean Orchestia during the molt cycle. J Exp Zool 249: 177–181

    Google Scholar 

  • Wecksler WR, Norman AV (1980) Biochemical properties of 1,25-dihydroxyvitamin D receptor. J Steroid Biochem 13: 977–991

    Google Scholar 

  • Weiner S, Noff D, Meyer MS, Weisman Y, Edelstein S (1979) Metabolism of cholecalciferol in land snails. Biochem J 184: 157–161

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biologie Animale, Faculté des Sciences, URA CNRS 674, 6 Boulevard Gabriel, F-21100, Dijon, France

    J. C. Meyran

  2. Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, URA CNRS 559, Oullins, France

    G. Morel

  3. Department of Biochemistry, University of Arizona, Tucson, Arizona, USA

    M. R. Haussler

  4. Faculté Alexis Carrel, U 234 INSERM, Lyon, France

    G. Boivin

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  1. J. C. Meyran
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  2. G. Morel
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  3. M. R. Haussler
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  4. G. Boivin
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Meyran, J.C., Morel, G., Haussler, M.R. et al. Immunocytological localization of 1,25-dihydroxyvitamin D3-like molecules and their receptors in a calcium-transporting epithelium of a crustacean. Cell Tissue Res 263, 345–351 (1991). https://doi.org/10.1007/BF00318776

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