Cell and Tissue Research

, Volume 261, Issue 2, pp 339–345 | Cite as

Immunohistochemical identification of calcitonin gene-related peptide and substance P in nerves of the bovine parathyroid gland

  • Shane T. Mortimer
  • David A. Hanley
  • William K. Stell
Article

Summary

Although peptide neurotransmitters have been shown to modulate hormone secretion in many glands, there are very few studies of neurotransmitters in the parathyroid gland. Bovine parathyroid glands were collected at a local abattoir, fixed with paraformaldehyde, sectioned using a cryostat, and stained by indirect immunohistochemistry for calcitonin gene-related peptide and substance P. We were able to positively identify both neuropeptides. Nerve fibres containing calcitonin gene-related peptide and substance P were identified in contact with the tunica media of arteries and arterioles and dispersed throughout the stroma of the gland. While many of the fibres encircled parenchymal lobules, no intimate contact with the peripheral chief cells was observed. All immunoreactive fibres were found to contain both neuropeptides. Since calcitonin gene-related peptide and substance P are vasodilators, they may increase blood flow within the gland. In addition, the neuropeptides may diffuse from perilobular nerve fibres into the parenchyma, thereby modulating secretion of parathyroid hormone.

Key words

Parathyroid glands Neuropeptide colocalization Calcitonin gene-related peptide (CGRP) Substance P Immunohistochemistry Cow 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Shane T. Mortimer
    • 1
  • David A. Hanley
    • 1
  • William K. Stell
    • 2
  1. 1.Department of Medicine, Faculty of MedicineThe University of CalgaryCalgaryCanada
  2. 2.Department of Anatomy, Faculty of MedicineThe University of CalgaryCalgaryCanada
  3. 3.Endocrine Research Group, Department of MedicineThe University of Calgary, Health Science CentreCalgaryCanada

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