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Autoradiographic localization of specific atrial natriuretic peptide binding sites on immunocytochemically identified cells in cultures from rat and guinea-pig hearts

Cell and Tissue Research volume 261, pages 301–312 (1990)Cite this article

Summary

Dissociated cell culture preparations from rat and guinea-pig atria and interatrial septum, and from rat ventricles were studied using a combined autoradiographic and immunocytochemical approach. Alphaatrial natriuretic peptide (125I-ANP1-128) binding sites were confined to subpopulations of identified non-neuronal cells in each type of culture preparation, and had distinct patterns of labelling. The density of ANP1-28 binding sites was substantially greater in guinea-pig cultures than in rat cultures and was least in rat ventricular cultures. ANP1-28-labelled subpopulations of S-100-like immunoreactive glial cells were only seen in guinea-pig cultures. Von Willebrand factor (vWF)-like immunoreactive endothelial cells and vWF-negative endothelioid cells expressed ANP1-28 binding sites in both the guinea-pig and rat atrial cultures, but were unlabelled in rat ventricular cultures. In contrast, labelled subpopulations of fibronectin-like immunoreactive fibroblasts were present in all of the three types of culture preparation studied. ANP-like immunoreactive myocytes were present in both atrial and ventricular cultures. These cells did not, however, express ANP1-28 binding sites.

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Affiliations

  1. Department of Anatomy and Developmental Biology, and Centre for Neuroscience, University College London, London, United Kingdom

    S. James, C. J. S. Hassall & G. Burnstock

  2. Department of Histochemistry, Royal Postgraduate Medical School, London, United Kingdom

    J. M. Polak

Authors
  1. S. James
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  2. C. J. S. Hassall
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  3. J. M. Polak
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  4. G. Burnstock
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James, S., Hassall, C.J.S., Polak, J.M. et al. Autoradiographic localization of specific atrial natriuretic peptide binding sites on immunocytochemically identified cells in cultures from rat and guinea-pig hearts. Cell Tissue Res 261, 301–312 (1990). https://doi.org/10.1007/BF00318671

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  • DOI: https://doi.org/10.1007/BF00318671

Key words

  • Atrial natriuretic peptide
  • Binding sites
  • Heart
  • Autoradiography
  • Cell culture
  • Rat
  • Guineapig