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Distribution of type-VII collagen in xenografted human carcinomas

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Abstract

The distribution of type-VII collagen, the main molecular component of the anchoring fibrils (AF) attaching the basal lamina (BL, lamina densa of the basement membrane) to the surrounding connective tissue, was investigated in four xenografted human carcinomas of the hypopharynx (H-Stg 1), the lung (L 261), the sigmoid colon (CA 1), and the rectum (R 85). The studies were performed with a recently prepared, affinity-purified and highly specific antibody to type-VII collagen by using the indirect immunofluorescence and the APAAP (alkaline phosphatase anti-alkaline phosphatase) techniques. For comparison, the localization of the intrinsic BL components laminin and type-IV collagen were additionally analyzed in all four carcinomas. It was shown that type-VII collagen usually colocalized to laminin and type-IV collagen and was deposited at the borderline between carcinoma cell clusters and the surrounding strands of connective tissue in a similar, but more diffuse and less continuous distribution than both intrinsic BL components. In the squamous cell carcinoma H-Stg 1 and the adenocarcinoma L 261, type-VII collagen was additionally accumulated in enlarged extracellular spaces between carcinoma cells, away from the contact zone to the connective tissue and again colocalized to laminin and type-IV collagen. Numerous carcinoma cells of both xenografts showed remarkable intracytoplasmic immunoreactivity for the antibody to type-VII collagen. Even in the case of the gastrointestinal carcinomas CA 1 and R 85, faint immunoreactivity for type-VII collagen was found at the contact zone between the mucosal epithelium and the surrounding connective tissue. These results confirm that epithelial carcinoma cells are obviously involved with the synthesis of the main molecular component of AF usally attaching the BL to the adjacent connective tissue and hint at a possible correlation between the localization of type-VII collagen and the observed pattern of the BL. However, it cannot be decided whether there is a direct causal relation between both phenomena or whether they are both the consequence of an independent but common cause, such as abnormal cellular differentiation of carcinoma cells. In no case, can the discontinuities in the distribution of type-VII collagen be explained by active tumor cell invasion since xenografted human carcinomas neither invade nor metastasize.

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References

  • d'Ardenne AJD (1989) Use of basement membrane markers in tumor diagnosis. J Clin Pathol 42:449–457

    Google Scholar 

  • Barrach HJ, Grundmann K, Hinz N, Felies A (1980) Immunofluorescent microscopic investigations of intercellular substances during limb development. In: Merker HJ, Nau H, Neubert D (eds) Teratology of the limbs. De Gruyter, Berlin, pp 273–293

    Google Scholar 

  • Barrach HJ, Grundmann K, Hinz N, Felies A (1981) Comparison of the differentiation of muscle and connective tissue of mouse limb bud in culture and in vivo: a morphological study by indirect immunofluorescence. In: Neubert D, Merker HJ (eds) Culture techniques. De Gruyter, Berlin, pp 135–159

    Google Scholar 

  • Biremaut P, Caron Y, Adnet JJ, Foidart JM (1985) Usefulness of basement membrane markers in tumoural pathology. J Pathol 145:283–296

    Google Scholar 

  • Bosman FT, Havenith M, Cleutjens JPM (1985) Basement membranes in cancer. Ultrastruct Pathol 8:291–307

    Google Scholar 

  • Briggeman RA, Dalldorf FG, Wheeler CE (1971) Formation and origin of basal lamina and anchoring fibrils in adult human skin. J Cell Biol 51:384–395

    Google Scholar 

  • Bruckner-Tuderman L, Mitsuhashi Y, Schnyder UW, Bruckner P (1989) Anchoring fibrils and type VII collagen are absent from skin in severe recessive dystrophic epidermolysis bullosa. J Invest Dermatol 93:3–9

    Google Scholar 

  • Burgeson RE, Lunstrum GP, Rokosova B, Rimberg CS, Rosenbaum LM, Keene DR (1990) The structure and function of type VII collagen. Ann NY Acad Sci 580:32–43

    Google Scholar 

  • Denker HW (1988) Implantation: recent approaches to understand a cell biological paradox. In: Iizuka R, Semm K (eds) Human reproduction, current status/future prospect. Elsevier, Amsterdam, pp 237–240

    Google Scholar 

  • Denker HW, Hohn HP, Bükers A (1989) Investigations on the cell biology of embryo implantation. Reproduct Biol Med 224–238

  • Ghadially R, Ghadially FN (1989) Anchoring fibrils in a spindle cell squamous carcinoma. J Submicrosc Cytol Pathol 21:249–257

    Google Scholar 

  • Gipson IK, Spurr-Michaud SJ, Tisdale AS (1988) Hemidesmosomes and anchoring fibril collagen appear synchronously during development and wound healing. Dev Biol 126:253–262

    Google Scholar 

  • Gusterson BA, Warburton MJ, Mitchell D, Kraft N, Hancock WW (1984) Invading squamous cell carcinoma can retain a basal lamina. An immunohistochemical study using a monoclonal antibody to type IV collagen. Lab Invest 51:82–87

    Google Scholar 

  • Hancock K, Tsang VCW (1983) India ink staining of proteins on nitrocellulose paper. Anal Biochem 133:157–162

    Google Scholar 

  • Hassell JR, Robey PG, Barrach HJ, Wilczek J, Rennard SI, Martin GR (1980) Isolation of a heparan sulfate-containing proteoglycan from basement membrane. Proc Natl Acad Sci USA 77:4494–4498

    Google Scholar 

  • Heagerty AHM, Kennedy AR, Leigh IM, Purkis P, Eady RAJ (1986) Identification of an epidermal basement membrane defect in recessive forms of dystrophic epidermolvsis bullosa by LH 7.2 monoclonal antibody: use in diagnosis. Br J Dermatol 115:125–131

    Google Scholar 

  • Keene DR, Sakai LY, Lunstrum GP, Morris NP, Burgeson RE (1987) Type VII collagen forms an extended network of anchoring fibrils. J Cell Biol 104:611–621

    Google Scholar 

  • Köpf-Maier P, Kestenbach U (1989) Host-supplied connective tissue as a guide for proliferating tumor cells in human tumor xenografts. Acta Anat 135:289–295

    Google Scholar 

  • Köpf-Maier P, Merker HJ (1989) Atlas der Elektronenmikroskopie. Ueberreuter Wissenschaft, Wien

    Google Scholar 

  • Köpf-Maier P, Merker HJ (1991) Development of the basal lamina in xenografted human carcinomas: an ultrastructural and immunohistochemical study. Cell Tissue Res 266:563–578

    Google Scholar 

  • Leblond CP, Inoue S (1989) Structure, composition, and assembly of basement membrane. Am J Anat 185:367–390

    Google Scholar 

  • Liotta LA (1984) Tumor invasion and metastases: role of the basement membrane. Am J Pathol 117:339–348

    Google Scholar 

  • Liotta LA, Tryggvason K, Garbisa S, Hart I, Foltz CM, Shafie S (1980) Metastatic potential correlates with enzymatic degradation of basement membrane collagen. Nature 284:67–68

    Google Scholar 

  • Liotta LA, Thorgeirsson UP, Garbisa S (1982) Role of collagenases in tumor cell invasion. Cancer Metastasis Rev 1:277–297

    Google Scholar 

  • Merker HJ, Bremer D, Barrach HJ, Gossrau R (1987) The basement membrane of the persisting maternal blood vessels in the placenta of Callithrix jacchus. Anat Embryol 176:87–97

    Google Scholar 

  • Paulsson M, Aumailley M, Deutzmann R, Timpl R, Beck K, Engel J (1987) Laminin-nidogen complex. Eur J Biochem 166:11–19

    Google Scholar 

  • Regauer S, Seiler GR, Barrandon Y, Easley KW, Compton CC (1990) Epithelial origin of cutaneous anchoring fibrils. J Cell Biol 111:2109–2115

    Google Scholar 

  • Richards CJ, Furness PN (1990) Basement membrane continuity in benign, premalignant and malignant epithelial conditions of the uterine cervix. Histopathology 16:47–52

    Google Scholar 

  • Romanos G, Schröter-Kermani C, Hinz N, Bernimoulin JP (1991) Immunohistochemical distribution of the collagen types IV, V, VI and glycoprotein laminin in the healthy rat, marmoset (Callithrix jacchus) and human gingivae. Matrix 11:125–132

    Google Scholar 

  • Sakai LY, KeeneDR, Morris NP, Burgeson RE (1986) Type VII collagen is a major structural component of anchoring fibrils. J Cell Biol 103:1577–1586

    Google Scholar 

  • Sinha AA, Gleason DF, Wilson MJ, Staley NA, Furcht LT, Palm SL, Reddy PK, Sibley RK, Martinez-Hernandez A (1989) Immunohistochemical localization of laminin in the basement membrane of normal, hyperplastic, and neoplastic human prostate. Prostate 15:299–313

    Google Scholar 

  • Thorgeirsson UP, Turpeenniemi-Hujanen T, Liotta LA (1985) Cancer cells, components of basement membranes, and proteolytic enzymes. Int Rev Exp Pathol 27:203–234

    Google Scholar 

  • Timpl R (1989) Structure and biological activity of basement membrane proteins. Eur J Biochem 180:487–502

    Google Scholar 

  • Trelstad R, Catanese V, Rubin D (1976) Collagen fractionation: separation of native types I, II, III by differential precipitation. Anal Biochem 71:114–118

    Google Scholar 

  • Tsubura A, Shikata N, Inui T, Morii S, Hatano T, Oikawa T, Matsuzawa A (1988) Immunohistochemical localization of myoepithelial cells and basement membrane in normal, benign and malignant human breast lesions. Virchows Arch [A] 413:133–139

    Google Scholar 

  • Van Den Hooff A (1989) An essay on basement membranes and their involvement in cancer. Persp Biol Med 32:401–413

    Google Scholar 

  • Wetzels RHW, Robben HCM, Leight IM, Schaafsma HE, Vooijs GP, Ramaekers FCS (1991) Distribution patterns of type VII collagen in normal and malignant human tissues. Am J Pathol 139:451–459

    Google Scholar 

  • Willebrand D, Bosman FT, De Goeij AFPM (1986) Patterns of basement membrane deposition in benign and malignant breast tumours. Histopathology 10:1231–1241

    Google Scholar 

  • Xu D, Schröter-Kermani C, Hinz N, Merker HJ (1990) Immunofluorescence-microscopic localization of collagen type IV and VI, laminin and nidogen in the livers of Callithrix jacchus during pre- and postnatal development. Acta Anat 138:212–219

    Google Scholar 

  • Zuk RJ, Baithun SI, Martin JE, Cox EL, Revell PA (1989) The immunocytochemical demonstration of basement membrane deposition in transitional cell carcinoma of bladder. Virchows Arch [A] 414:447–452

    Google Scholar 

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Authors and Affiliations

  1. Institut für Anatomie, Freie Universität Berlin, Königin-Luise-Strasse 15, W-1000, Berlin 33, Germany

    P. Köpf-Maier

  2. Institut für Toxikologie und Embryonalpharmakologie, Frete Universität Berlin, W-1000, Berlin 33, Germany

    C. Schröter-Kermani

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  1. P. Köpf-Maier
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  2. C. Schröter-Kermani
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Köpf-Maier, P., Schröter-Kermani, C. Distribution of type-VII collagen in xenografted human carcinomas. Cell Tissue Res 272, 395–405 (1993). https://doi.org/10.1007/BF00318546

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  • DOI: https://doi.org/10.1007/BF00318546

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