Current Genetics

, Volume 20, Issue 4, pp 319–329 | Cite as

The first analysed archegoniate mitochondrial gene (COX3) exhibits extraordinary features

  • Joachim R. Marienfeld
  • Ralf Reski
  • Wolfgang O. Abel
Original Articles


The first mitochondrial-encoded gene of an archeogoniate has been identified, cloned and sequenced. The cytochrome oxidase III gene (cox3) of the moss Physcomitrella patens consists of a 618 bp open reading frame with high homology (around 72%) to known cox3 sequences of higher plants. Nevertheless, it is a quarter shorter than these. The cox3 gene of P. patens contains no introns and reveals a G+C-content of 41.3%. The region containing the cox3 gene exists as a single copy in the mitochondrial genome as shown by restriction mapping. In the 5′ flanking sequence a putative ribosome binding site and a putative secondary structure were found. Two main transcripts of 2.4 kb and 2.6 kb were detected indicating a complex mitochondrial transcription pattern possibly due to co-transcription. Additional open reading frames were found downstream from, as well as upstream of, the cox3 gene. In Western blots a polyclonal cox3 antibody from yeast detected one single band with an apparent molecular weight of 22 kDa.

Key words

Lower plant mitochondrial DNA Cytochrome oxidase subunit III gene Moss (Physcomitrella patens


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Joachim R. Marienfeld
    • 1
  • Ralf Reski
    • 1
  • Wolfgang O. Abel
    • 1
  1. 1.Institut für Allgemeine BotanikUniversität HamburgHamburg 52Federal Republic of Germany

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