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Uptake and transport of copolymer biodegradable microspheres by rabbit Peyer's patch M cells

Cell and Tissue Research volume 279, pages 433–436 (1995)Cite this article

Abstract

In this study, we demonstrate the role of M cells in uptake of poly(D-L-lactic-co-glycolic acid) (PLGA) microspheres and transport into rabbit Peyer's patches. Microspheres 1 to 10 μm in diameter composed of 50:50 lactic acid:glycolic acid were instilled into in-testinal segments containing jejunal or ileal Peyer's patches, and uptake by M cells was examined by electron microscopy. PLGA microspheres visualized as electron-lucent, spherical particles were taken up by M cells by pseudopod-like extensions of the M cell apical membrane and translocated to the pocket region containing mononuclear leukocytes within 60 min. These results indicate that PLGA microspheres can be directed to M cell apical surfaces for delivery to immunocompetent cells in gut-associated lymphoid tissues.

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Affiliations

  1. Vaccine Delivery Research Section, Ora Vax Inc., 230 Albany Street, 02139, Cambridge, MA, USA

    Thomas H. Ermak, Edward P. Dougherty, Zita Kabok & Jacques Pappo

  2. Formulations Research Section, Ora Vax Inc., 230 Albany Street, 02139, Cambridge, MA, USA

    Hitesh R. Bhagat

Authors
  1. Thomas H. Ermak
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  2. Edward P. Dougherty
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  3. Hitesh R. Bhagat
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  4. Zita Kabok
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  5. Jacques Pappo
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Ermak, T.H., Dougherty, E.P., Bhagat, H.R. et al. Uptake and transport of copolymer biodegradable microspheres by rabbit Peyer's patch M cells. Cell Tissue Res 279, 433–436 (1995). https://doi.org/10.1007/BF00318501

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  • DOI: https://doi.org/10.1007/BF00318501

Key words

  • Peyer's patches
  • Microspheres
  • Epithelium
  • M cells
  • Rabbit