Summary
An immunohistochemical study using antiserum raised against synthetic chicken natriuretic polypeptide was used to investigate the distribution of this peptide in the chicken heart. Immunoreactive cells, both in the atrial and ventricular walls, were identified by electron microscopy, and electron-dense granules in the atrial and ventricular cardiocytes were revealed to be storage sites of the peptide. The electron-dense material, thought to be the peptide, was found in the sarcoplasmic reticulum, and it is suggested that a secretory pathway of the peptide through the latter to extracellular space, may be present, in addition to an exocytotic one.
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References
Bencosme SA, Berger JM (1971) Specific granules in mammalian and non-mammalian vertebrate cardiocytes. In: Bajusz E, Jasmin G (eds) Functional morphology of the hearts. Methods Achiev Exp Pathol, Vol 5. Karger, Basel, pp 173–213
Bold A de, Salerno TA (1983) Natriuretic activity of extracts obtained from hearts of different species and from various rat tissues. Can J Physiol Pharmacol 61:127–130
Chapeau C, Gutkowska J, Schiller PW, Milne RW, Thibault G, Garcia R, Genest J, Cantin M (1985) Localization of immunoreactive synthetic atrial natriuretic factor (ANF) in the heart of various animal species. J Histochem Cytochem 33:541–550
Guesdon J, Ternynck T, Avrameas S (1979) The use of avidinbiotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139
Horisberger M (1981) Colloidal gold: Acytochemical marker for light and fluorescent microscopy and for transmission and scanning electron microscopy. Scanning Electron Microsc 2:9–31
Hsu S, Raine L, Fanger H (1981) Use of avidin-biotin peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Miyata A, Kangawa K, Toshimori T, Hatoh T, Matsuo H (1985) Molecular forms of atrial natriuretic polypeptides in mammalian tissues and plasma. Biochem Biophys Res Commun 129:248–255
Miyata A, Minamino N, Kangawa K, Matsuo H (1988) Identification of a 29-amino acid natriuretic peptide in chicken heart. Biochem Biophys Res Commun 155:1330–1337
Netchitailo P, Feuilloley M, Pelletier G, Cantin M, De Lean A, Leboulenger F, Vaudry H (1986) Localization and characterization of atrial natriuretic factor (ANF)-like peptide in the frog atrium. Peptides 7:573–579
Reinecke M, Nehls M, Forssmann WG (1985) Phylogenetic aspects of cardiac hormones as revealed by immunocytochemistry, electron microsocpy, and bioassay. Peptides 6:321–331
Reinecke M, Betzler D, Forssman WG (1987) Immunocytochemistry of cardiac polypeptide hormones (cardiodilatin/atrial natriuretic polypeptide) in brain and hearts of Myxine glutinosa (Cyclostomata). Histochemistry 86:233–239
Sakata J, Kangawa K, Matsuo H (1988) Identification of new atrial natriuretic peptides in frog heart. Biochem Biophys Res Commun 155:1338–1345
Schiebinger RJ, Linden J (1986) Effect of atrial contraction frequency on atrial natriuretic peptide secretion. Am J Physiol 251:H1095-H1099
Sommer JR, Johnson EA (1969) Cardiac muscle. A comparative ultrastructural study with special reference to frog and chicken hearts. Z Zellforsch 98:437–468
Sommer JR, Johnson EA (1970) Comparative ultrastructure of cardiac cell membrane specializations. A review (1970). Am J Cardiol 25:184–194
Sudoh T, Kangawa K, Minamino N, Matsuo H (1988) A new natriuretic peptide in porcine brain. Nature 332:78–81
Sugawara M (1987) Ultrastructural evidence of exocytotic release of atrial natriuretic polypeptide in the rat atrial muscle with the use of the tannic acid method. Biomed Res 8:9–17
Toshimori H, Toshimori K, Oura C, Matsuo H (1987a) Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, fetal and neonatal rat heart. Cell Tissue Res 248:627–633
Toshimori H, Toshimori K, Oura C, Matsuo H (1987b) Immunohistochemistry and immunocytochemistry of atrial natriuretic polypeptide in porcine heart. Histochemistry 86:595–601
Toshimori H, Toshimori K, Oura C, Matsuo H, Matsukura S (1988a) The distribution of atrial natriuretic polypeptide (ANP)-containing cells in the adult rat heart. Anat Embryol 177:477–484
Toshimori H, Toshimori K, Oura C, Matsuo H, Matsukura S (1988b) Immunohistochemical identification of Purkinje fibers and transitional cells in a terminal portion of the impulse conducting system of porcine heart. Cell Tissue Res 253:47–53
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Toshimori, H., Toshimori, K., Minamino, N. et al. Chicken atrial natriuretic peptide (chANP) and its secretion. Cell Tissue Res 259, 293–298 (1990). https://doi.org/10.1007/BF00318451
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DOI: https://doi.org/10.1007/BF00318451