Summary
This study demonstrates that endocytosis in the oocyte of Drosophila melanogaster is reversibly blocked at the stage of pit formation by the temperature-sensitive, single-gene mutant, shibire ts1. Uptake of horseradish peroxidase conjugated with wheat-germ agglutinin was observed to be normal in mutant oocytes at 19°C, but was blocked at 29°C. After 10 min at 29°C, there was a build-up of coated pits along invaginations of the plasma membrane. Also, the endosomal compartment consisting of tubules, bulbs, and small yolk spheres, disappeared. Lowering the temperature to 19°C after 10 min at 29°C released a synchronized wave of endocytosis into a cytoplasm cleared of uptake-related organelles. By observing this synchronized wave after exposure to 19°C for varying durations, we determined that endocytosis proceeds as follows: coated pits/vesicles→tubules→small yolk spheres→mature yolk spheres. The observations suggest that these organelles transform one into another within this sequence.
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Tsuruhara, T., Koenig, J.H. & Ikeda, K. Synchronized endocytosis studied in the oocyte of a temperature-sensitive mutant of Drosophila melanogaster . Cell Tissue Res 259, 199–207 (1990). https://doi.org/10.1007/BF00318441
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DOI: https://doi.org/10.1007/BF00318441