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Fluorescence microscopic study of the behaviour of DANS-marked histidine in the rat brain after intraventricular injection

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Summary

Following stereotactic injection of dansyl-histidine into the third ventricle of the rat, uptake and further transport of the amino acid in respect of the ependyma, the choroid plexus and the brain were investigated with fluoresence microscopy, within the framework of a time study.

  1. 1.

    Ependyma. The labelled amino acid, which emits a yellow autofluorescence, is taken up very rapidly by the ependyma and 5 min after injection is demonstrable in the whole of the ventricular lining, the boundary with the subjacent brain being sharply demarcated. In respect of subsequent transport there are regional differences. After 60 min p.i. the histidine has disappeared from some sections of the ependyma. The ependymal lining of the third ventricle is totally depleted 90 min post injectionem, that of the lateral ventricles 60 and 120 min p.i., that of the aqueduct 40 min p.i., and that of the fourth ventricle 60 min p.i.

  2. 2.

    Choroid Plexus. At the earliest time of observation permitted by dissecting out etc., all plexuses are found to have taken up histidine. Duration of storage, however, varies. While the plexus of the third ventricle is already depleted in stretches after 60 min and totally depleted after 120 min, the fluorescent amino acid is still discernible in the plexuses of the fourth and lateral ventricles after 180 min.

  3. 3.

    Brain. Penetration of the amino acid into the cerebral tissue commences 10 min p.i. and lasts, taking into account regional temporal differences, 180 min. The histidine becomes localized foremostly in the following areas: nucl. anterior hypothalami, nucl. ventromedialis hypothalami, nucl. praeopticus, nucl. paraventricularis, stria terminalis, stria medullaris thalami and nucl. septi lateralis. In the cerebellum, DH-storing cells occur only in the molecular layer.

The results of this investigation are compared with transport routes and deposition centres relevant to tryptophan and phenylalanine, which have been studied using the same methodology. Whereas the latter amino acids show definite affinities for serotinergic and dopaminergic nuclear areas, histidine chiefly permeates periventricular structures and hypothalamic nuclear areas.

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  1. Fachrichtung Anatomie im Fachbereich Theoretische Medizin der Universität des Saarlandes, Homburg/Saar, Germany

    Karl Heinz Booz & Bernd Wiesen

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  1. Karl Heinz Booz
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  2. Bernd Wiesen
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Heinz Booz, K., Wiesen, B. Fluorescence microscopic study of the behaviour of DANS-marked histidine in the rat brain after intraventricular injection. Anat. Embryol. 149, 225–239 (1976). https://doi.org/10.1007/BF00317899

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