Summary
CO2 exchange and fluorescence yield of the crustose lichen Buellia frigida were measured in situ by means of a CO2 porometer and a PAM-2000, a newly developed portable fluorescence system. The pulse amplitude modulation system of the PAM-2000 allows measurements in the field under ambient light, temperature and moisture conditions without dark adaptation of the sample. CO2 exchange and fluorescence measurements were well correlated when measured under natural conditions in continental Antarctica during a drying cycle of melt-water-soaked lichen thalli. It was shown that the fluorescence parameter ΔF/Fm′ is a measure of the photosynthetic activity of the lichen. It proved possible, using the PAM-2000, to differentiate the physiological performance of the thallus centre and the marginal lobes. The distribution of water in the thallus during a drying cycle was shown to be inhomogeneous. The photosynthetic rates of B. frigida calculated on an area basis are comparatively high and indicate that this lichen is well adapted to its habitat conditions in this part of continental Antarctica.
Similar content being viewed by others
Abbreviations
- Fm′:
-
maximum yield of fluorescence under ambient light
- Fs:
-
yield of fluorescence at steady state
- ΔF :
-
difference in yield of fluorescence at maximal Fm′ and steady state Fs under ambient light
- NP:
-
net photosynthesis
- PAR:
-
photosynthetically active radiation
- PS II:
-
photosystem II
References
Bilger W, Rimke S, Schreiber U, Lange OL (1989) Inhibition of energy-transfer to photosystem II in lichens by dehydration: different properties of reversibility with green and blue-green phycobionts. J Plant Physiol 134:261–268
Bolhàr-Nordenkampf HR, Long SP, Baker NR, Öquist G, Schreiber U, Lechner EG (1989) Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation. Funct Ecol 3:497–514
Genty B, Briantais JM, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990:87–92
Green TGA, Lange OL (1991) Ecophysiological adaptations of the lichen genera Pseudocyphellaria and Sticta to south temperate rainforests. Lichenologist 23:267–282
Jensen M, Feige GB (1991) Quantum efficiency and chlorophyll fluorescence in the lichens Hypogymnia physodes and Parmelia sulcata. Symbiosis 11:179–191
Kappen L (1988) Ecological and physiological relationships in different climatic regions. In: Galun M (ed) Handbook of lichenology Vol. II. CRC Press, Boca Raton, pp 37–100
Kappen L, Friedmann EI (1983) Ecophysiology of lichens in the Dry Valleys of southern Victoria Land, Antarctica. II: CO2 gas exchange in cryptoendolithic lichens. Polar Biol 1:227–232
Kappen L, Schroeter B, Sancho LG (1990) Carbon dioxide exchange of Antarctic crustose lichens in situ measured with a CO2/H2O porometer. Oecologia 82:311–316
Kershaw KA (1985) Physiological ecology of lichens. Cambridge University Press, Cambridge, p 293
Lange OL, Kappen L (1972) Photosynthesis of lichens from Antarctica. In: Llano GA (ed) Antarctic terrestrial biology. Vol. 20 American Geophysiological Union, Washington, pp 83–95
Lange OL, Matthes (1981) Moisture-dependent CO2 exchange of lichens. Photosynthetica 15:555–574
Lange OL, Schulze ED, Koch W (1970) Experimentell-ökologische Untersuchungen an Flechten der Negev-Wüste. III. CO2-Gaswechsel und Wasserhaushalt von Krusten- und Blattflechten am natürlichen Standort während der sommerlichen Trockenperiode. Flora 159:525–538
Lange OL, Bilger W, Rimke S, Schreiber U (1989) Chlorophyll fluorescence of lichens containing green and blue-green algae during hydration by water vapor uptake and by addition of liquid water. Bot Acta 102:305–313
Lange OL, Meyer A, Ullmann I, Zellner H (1991) Mikroklima, Wassergehalt und Photosynthese von Flechten in der küstennahen Nebelzone der Namib-Wüste: Messungen während der herbstlichen Witterungsperiode. Flora 185:233–266
Ried A (1960) Stoffwechsel und Verbreitungsgrenzen von Flechten. II. Wasser- und Assimilationshaushalt, Entquellungs- und Submersionsresistenz von Krustenflechten benachbarter Standorte. Flora 149:345–385
Schreiber U (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll-fluorimeter. Photosyn Res 9:261–272
Schreiber U, Neubauer C (1990) O2-dependent electron flow, membrane energization and the mechanism of non-photochemical quenching of chlorophyll fluorescence. Photosyn Res 25:279–293
Schreiber U, Reising H, Neubauer C (1991) Contrasting pH-optima of light-driven O2- and H2O2-reduction in spinach chloroplasts as measured via chlorophyll fluorescence quenching. Z Naturforsch 46c:635–643
Schroeter B (1991) Untersuchungen zu Primärproduktion und Wasserhaushalt von Flechten der maritimen Antarktis unter besonderer Berücksichtigung von Usnea antarctica DuRietz. Dissertation Universität Kiel
Schroeter B, Jacobsen P, Kappen L (1991a) Thallus moisture and microclimatic control of the CO2-exchange of Peltigera aphthosa (L.) Willd.on Disko Island (West Greenland). Symbiosis 11:131–146
Schroeter B, Kappen L, Moldaenke C (1991b) Continuous in situ recording of the photosynthetic activity of Antarctic lichens —established methods and a new approach. Lichenologist 23:253–265
van Kooten O, Snel JFH (1990) The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosyn Res 25:147–150
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Dr. W. Nultsch, Marburg, on the event of his 65th birthday
Rights and permissions
About this article
Cite this article
Schroeter, B., Green, T.G.A., Seppelt, R.D. et al. Monitoring photosynthetic activity of crustose lichens using a PAM-2000 fluorescence system. Oecologia 92, 457–462 (1992). https://doi.org/10.1007/BF00317836
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00317836