Summary
Drosophila males were injected with a 0.008 M solution of potassium cyanide prior to X-radiation with doses varying from 980–2400 r. Compared to the controls, which were only X-rayed, the frequency of sex-linked lethals after cyanide pretreatment was significantly increased in germ cells which formed mature sperm 4–7 days after treatment. A similar enhancing effect on the X-ray induced mutation rate was obtained by pretreatment with sodium azide. The results are tentatively ascribed to an increased production of hydrogen peroxide in the pretreated irradiated germ cells.
Zusammenfassung
Vor einer Röntgenbestrahlung, deren Stärke von 980–2400 r variierte, wurden Drosophila-Männchen mit einer 0.008 Molar Kaliumzyanidlösung injiziert. Es stellte sich heraus, daß im Vergleich zu den Kontrollen, welche nur bestrahlt wurden, die Mutationsrate nach Zyanidvorbehandlung signifikant gesteigert war, und zwar in den jüngeren Geschlechtszellen, welche 4–7 Tage nach der Behandlung reife Spermatozoen gebildet hatten. Eine ähnliche Zunahme der von Röntgenstrahlen induzierten Mutationsrate konnte nach Vorbehandlung mit Natriumazid beobachtet werden. Als ein möglicher Grund für diese Beobachtungen wird ein erhöhter Gehalt an Wasserstoffsuperoxyd in den vorbehandelten, bestrahlten Zellen in Erwägung gezogen.
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Sobels, F.H. The effect of pretreatment with cyanide and azide on the rate of X-ray induced mutations in Drosophila . Z.Ver-erbungslehre 86, 399–404 (1955). https://doi.org/10.1007/BF00317448
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DOI: https://doi.org/10.1007/BF00317448