Summary
The nature of acetylated Sudan Black B (aSBB) has been investigated, and it has been found, by thin layer chromatography, that each fraction of aSBB has an R f which is the same as that of a similar fraction of Sudan Black B (SBB). However, aSBB has been found to have fewer fractions, 9–12 than SBB, 14–16. The two major fractions from aSBB and SBB were examined, and a great similarity was found between the absorption spectra of the respective fractions of aSBB and SBB. The major fraction of aSBB was investigated by mass spectroscopy and found to have a similar molecular weight to that expected of SBB. This demonstrates that aSBB is not in fact acetylated, and that the components of aSBB are chemically no different from the corresponding components of SBB.
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References
Bermes EW Jr, McDonald HJ (1957) Fractionation and characterization of the lipide stain, Sudan Black B. Arch Biochem Biophys 70:49–57
Beynon AD (1976) A reappraisal of Sudan Black staining of enamel matrix in the mouse molar. Arch Oral Biol 21:83–90
Brock B, Stowell RE, Couch K (1952) Staining of intranuclear lipids of mouse liver under different conditions of growth. Lab Invest 1:439–446
Casselman WGB (1954) Acetylated Sudan Black B as a more specific histochemical reagent for lipides. Q J Microsc Sci 92:321–322
Franz H, Pfüller U, Glöckner R (1977) Ein Verfahren zur Reindarstellung der beiden Hauptkomponenten von Sudanschwarz B. Zentralbl Allg Pathol 121:489–494
Frederiks WM (1977) Some aspects of the value of Sudan Black B in lipid histochemistry. Histochemistry 54:27–37
Fredricsson B (1958) Staining of freeze dried tissue with Sudan Black B after embedding in polyethylene glycol. Acta Morphol Neerl Scand 1:377–383
Fredricsson B, Laurent TC, Lüning B (1958) Decomposition of Sudan Black causing an artifact in the staining of lipids. Stain Technol 33:155–158
Horobin RW, Proctor GB (1984) The case of the colourblind analyst. Proc R Microsc Soc 19:37 (Abst)
Lansink AGW (1968) Thin layer chromatography and histochemistry of Sudan Black B. Histochemie 16:68–84
Lillie RD (1965) Histopathological technic and practical histochemistry. 3rd edn. McGraw Hill, New York, pp 588–590
Lillie RD, Burtner HJ (1953) Stable sudanophilia of human neutrophil leucocytes in relation to peroxidase and oxidase. J Histochem Cytochem 1:8–26
Lison L (1934) Sur de nouveaux colorants histologiques specifiques des lipides. CR Soc Biol (Paris) 115:202–205
Marshall PN (1977) Thin layer chromatography of Sudan dyes. J Chromatogr 136:353–357
Pfüller U, Franz H, Preiß A (1977) Sudan Black B: Chemical structure and histochemistry of the blue main components. Histochemistry 54:237–250
Schott HJ (1964) Zur Frage der histochemischen Fettspezifität des Sudanschwarz B und zur Methodik der Auftrennung seiner Fraktionen. Histochemie 3:467–476
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Lauder, R.M., Beynon, A.D. Evidence against the existence of acetylated Sudan Black B. Histochemistry 93, 213–216 (1989). https://doi.org/10.1007/BF00315977
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DOI: https://doi.org/10.1007/BF00315977