Summary
When in chick embryos (H.-H. stages 22 to 25) a variously large area of ectoderm with the subjacent mesodermal layer external to the superficial vessel network, loosened from the dorsal face of the wing bud is rotated 180° in situ, or a similar ecto- and mesodermal sheet isolated from the dorsal face of the leg bud is grafted, in normal of 180° reversed orientation, onto the dorsal face of the wing bud, no changes in the normal developmental pattern of the wing skeleton ensue. As the grafted tissue, which apparently does not contain prospective chondrogenic cells, develops as a flat implant, the normal geometry of the ectodermal hull is not altered: therefore, the biomechanical conditions and the polarized growth of the skeletogenous mesenchyme of the wing bud, which seem to be controlled by the enveloping epithelium, remain practically unchanged.
Morphological alterations of the skeletal pieces of the wing and formation of ectopic cartilage follow instead the implantation on the dorsal face of the wing bud, in normal or 180° reversed orientationm of an ecto- and mesodermal sheet similar to the one mentioned above but containing also a varying amount of the mesenchyme lying beneath the superficial vessel network.
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References
Amprino, R.: Aspects of limb morphogenesis in the chicken. In: Organogenesis (R.L. DeHaan and H. Ursprung, eds.). New York: Holt, Rinehart and Winston 1965
Amprino, R.: Possibile ruolo della topo-inibizione nello sviluppo normale e nei processi di regolazione di territori embrionali. Boll. Soc. Ital. Biol. sper. 49, 148–154 (1973)
Amprino, R.: On the topography of the presumptive skeletal mesenchyme in the early wing bud of chick embryos. Arch. Biol. (Bruxelles) 87, 1–41 (1976)
Amprino, R.: Experimental study on the morphogenetic relationships between ectoderm and mesoderm in the developing chick embryo limb bud. Acta Embryol. exper. 1, 51–70 (1977a)
Amprino, R.: Morphogenetic interrelationships between ectoderm and mesoderm in chick embryo limb development. In: Vertebrate limb and somite development (D. A. Ede, M. Balls and J. Hinchliffe, eds.) Cambridge: University Press 1977b
Amprino, R., Aiello Malmberg, P.: Observations on the avascular cultures of the chick embryo limb bud. Acta Embryol. exper. 3–28 (1973)
Amprino, R., Ambrosi, G.: Experimental analysis of the chick embryo limb bud growth. Arch. Biol. (Bruxelles) 84, 35–86 (1973)
Amprino, R., Camosso, M.E.: Analisi sperimentale dello sviluppo dell'ala nell'embrione di pollo. Arch. Entwicklsmech. Org. 150, 509–541 (1958)
Amprino, R., Camosso, M.E.: Developmental fate of heterotopically grafted proximal pre-axial material of the chick embryo limb bud. Acta Anat. 61, 259–288 (1965)
Gumpel-Pinot, M.: Culture in vitro de l'ébauche de l'aile de l'embryon de Poulet. Rôle de l'ectoderme sur la chondrogenèse. C.R. Acad. Sci. Paris 274, 2786–2789 (1972)
Gumpel-Pinot, M.: Culture in vitro du bourgeon d'aile d'embryon de poulet: différenciation du cartilage. Ann. biol. Paris 12, 417–429 (1973)
Jorquera, B., Pugin, E.: Sur le comportement du mésoderme et de l'ectoderme du bourgeon de membre dans les échanges entre le Poulet et le Rat. C.R. Acad. Sci. Paris 272, 1522–1525 (1971)
MacCabe, J.A., Errick, J., Saunders, J.W., Jr.: Ectodermal control of the dorsoventral axis in the leg bud of the chick embryo. Develop. Biol. 39, 69–82 (1974)
MacCabe, J.A., Saunders, J.W., Jr., Pickett, M.: The control of the anteroposterior and dorsoventral axes in embryonic chick limbs constructed of dissociated and reaggregated limb-bud mesoderm. Develop. Biol. 31, 323–335 (1973)
Milaire, J.: Indices d'une participation morphogène de l'épiblaste au cours du développement des membres chez les mammifères. Arch. Biol. (Bruxelles) 84, 87–114 (1973)
Milaire, J., Mulnard, J.: Le rôle de l'épiblaste dans la chondrogenèse du bourgeon de membre chez la souris. J. Embryol. exp. Morphol. 20, 215–236 (1968)
Rubin, L., Saunders J.W., Jr.: Ectodermal-mesodermal interactions in the growth of limb buds in the chick embryo: constancy and temporal limits of the ectodermal induction. Develop. Biol. 28, 94–112 (1972)
Seichert, V., Rychter, Z.: Vascularization of the developing anterior limb of the chick embryo. I. Sinus marginalis, its development, fate and importance. Folia Morphol. (Praha) 19, 367–377 (1971)
Searls, R.L.: Effect of dorsal and ventral limb ectoderm on the development of the limb of the embryonic chick. J. Embryol. exp. Morphol. 35, 369–381 (1976)
Stark, R.J., Searls, R.L.: The establishment of the cartilage pattern in the embryonic chick wing, and evidence of a role of the dorsal and ventral ectoderm in normal wing development. Develop. Biol. 38, 51–63 (1974)
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Amprino, R. Relationships between ectoderm and skeletal morphogenesis in the chick embryo limb bud. Anat. Embryol. 153, 305–320 (1978). https://doi.org/10.1007/BF00315933
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DOI: https://doi.org/10.1007/BF00315933