Summary
Using single and double labelling techniques respectively, brain-corpora cardiaca-corpora allata complexes of the cockroach Blattella germanica have been immunohistochemically investigated with antisera raised against either the vertebrate peptide gastrin-cholecystokinin (CCK-8(s)) and/or the locust neurohormone neuroparsin (NPA).
Single immunolabelling with anti-CCK-8(s) reveals immunoreactive perikarya and processes in median and lateral parts of protocerebrum, optic lobes, deutocerebrum and tritocerebrum. Some fibres originating in median and lateral protocerebrum are intrinsic to the brain, whereas others terminate in the nervous areas of the corpora cardiaca. Single immunolabelling with anti-NPA reveals immunoreactive cell bodies in the median part of the protocerebrum and their processes terminate both in the nervous area of the corpora cardiaca and between the intrinsic secretory cells of this neurohaemal organ. Double immunolabelling with anti-CCK-8(s) and anti-NPA enables a description of the anatomical relations between the processes and the endings of these two neurosecretory systems.
Similar content being viewed by others
References
Bellés X, Piulachs MD (1983) Desarrollo de los corpora allata, oocitos y glandulas colaterales durante el primer ciclo gonotrofico de Blattella germanica. Rev Esp Fisiol 39:149–154
Bourême D, Tamarelle M, Girardie J (1987) Production and characterization of antibodies to neuroparsins A and B isolated from the corpora cardiaca of the locust. Gen Comp Endocrinol 67:169–177
Bourême D, Fournier B, Matz G, Girardie J (1989) Immunological and functional cross-reactivities between locust neuroparsins and proteins from cockroach corpora cardiaca. J Insect Physiol 35:265–271
Dhainaut-Courtois N, Tramu G, Marcel R, Malecha J, Verger-Bocquet M, Andries JC, Masson M, Selloun L, Bellemtougri G, Beauvillain JC (1984) Cholecystokinin in the nervous systems of invertebrates and protochordates immunohistochemical localization of a cholecystokinin-S-like substance in annelids and insects. In: Vanderhaeghen JJ (ed) Neuronal cholecystokinin Ann NY Acad Sci 448:167–187
Duve H, Thorpe A (1981) Gastrin/cholecystokinin (CCK)-like immunoreactive neurons in the brain of the blowfly, Calliphora erythrocephala (Diptera). Gen Comp Endocrinol 43:381–391
Ganagarajah M, Saleuddin ASM (1970) A simple manoeuvre to prevent loss of sections for performic acid-Victoria blue technique and a comparison of various neurosecretory stains. Can J Zool 48:1437–1458
Girardie J, Bourême D, Girardie A (1986) Production sites of the three neurosecretory proteins characterized in the corpora cardiaca of the migratory locust. Insect Biochem 17:29–36
Hansen GN, Hansen BL, Scharrer B (1987) Gastrin/CCK-like immunoreactivity in the corpus cardiacum-corpus allatum complex of the cockroach Leucophaea maderae. Cell Tissue Res 248:595–598
Nachman RJ, Holman MG, Haddon WF, Ling N (1986a) Leucosulfakinin, a sulfated insect neuropeptide with homology to gastrin and cholecystokinin. Science 234:71–73
Nachman RJ, Holman GM, Cook BJ, Haddon WF, Ling N (1986b) Leucosulfakinin II, a blocked sulfated insect neuropeptide with homology to cholecystokinin and gastrin. Biochem Biophys Res Commun 140, 1:347–364
Nakane PK (1968) Simultaneous localization of multiple tissue antigens using the peroxidase-labelled antibody method: a study on pituitary glands of the rat. J Histochem Cytochem 16:557–560
Pipa RL (1978) Locations and central projections of neurons associated with the retrocerebral neuroendocrine complex of the cockroach Periplaneta americana (L.). Cell Tissue Res 193:443–455
Sternberger LA, Hardy JPH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Sternberger LA (1986) Immunocytochemistry, 3rd edn. Wiley, New York
Tamarelle M, Girardie J (1989) Immunohistochemical investigation of locust neuroparsin-like substances in several insects, in some other invertebrates, and vertebrates. Histochemistry 91:431–435
Tamarelle M, Romeuf M, Vanderhaeghen JJ (1988) Immunohistochemical localization of gastrin-cholecystokinin-like material in the central nervous system of the migratory locust. Histochemistry 89:201–207
Vanderhaeghen JJ, Lotstra F, De Mey J, Gilles C (1980) Immunohistochemical localization of cholecystokinin and gastrin-like peptide in the brain and hypophysis of the rat. Proc Natl Acad Sci USA 77:1190–1194
Vanderhaeghen JJ, Signeau JC, Gepts W (1975) New peptide in the vertebrate CNS reacting with anti-gastrin antibodies. Nature 257:604–605
Vandesande F (1988) Double and multiple immunoenzymatic labelling of tissue sections for light microscopy. Acta Histochemica 35:107–115
Yui R, Fujita T, Ito S (1980) Insulin-, gastrin-, pancreatic polypeptide-like immunoreactive neurons in the brain of the silkworm, Bombyx mori. Biomed Res 1:42–46
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tamarelle, M., Bellés, X., Piulachs, D. et al. Gastrin-cholecystokinin-like and neuroparsin-like immunoreactivities in the brain and retrocerebral neuroendocrine complex of the cockroach Blattella germanica . Histochemistry 93, 433–438 (1990). https://doi.org/10.1007/BF00315863
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00315863