Abstract
Genomic clones hybridizing to anonymous, single-copy sequences were used to probe chromosomesized DNAs of Fusarium oxysporum f. sp. cubense separated by pulsed-field gel electrophoresis. As expected, most clones hybridized to single chromosome bands. However, two of eight “single-copy” clones hybridized to two chromosomes in some, but not all, of 14 isolates examined. This observation suggests a degree of genetic duplication in the fungus and is consistent with recent electrophoretic karyotype analysis indicating that intraspecific differences in genome size and chromosome number may be explained, at least in part, by persistent genetic duplication.
Similar content being viewed by others
References
Boehm EWA, Ploetz RC, Kistler HC (1994) Statistical analysis of electrophoretic karyotype variation among vegetative compatibility groups of Fusarium oxysporum f. sp. cubense. Mol Plant-Microbe Interact 7:196–207
Daboussi MJ, Langin T (1994) Transposable elements in the fungal plant pathogen Fusarium oxysporum. Genetica 93:49–59
Elias KS, Zamir D, Lichtman-Pleban T, Katan T (1993) Population structure of Fusarium oxysporum f. sp. lycopersici: restriction fragment length polymorphisms provide genetic evidence that vegetative compatibility group is an indicator of evolutionary origin. Mol Plant-Microbe Interact 6:565–572
Fekete C, Nagy R, Debets AJM, Hornok L (1993) Electrophoretic karyotypes and gene mapping in eight species of the Fusarium sections Arthrosporiella and Sporotrichiella. Curr Genet 24:500–504
Fincham JRS, Day PR, Radford A (1979) Fungal genetics, 4th edn. University of California Press, Berkeley
Kim DH, Martyn RD, Magill CW (1993) Chromosomal polymorphism in Fusarium oxysporum f. sp. niveum. Phytopathology 83:1209–1216
Kistler HC, Miao VPW (1992) New modes of genetic change in filamentous fungi. Annu Rev Phytopathol 30:131–152
McCluskey K, Mills D (1990) Identification and characterization of chromosome length polymorphism among strains representing fourteen races of Ustilago hordei. Mol Plant-Microbe Interact 3:366–373
McDonald BA, Martinez JP (1991) Chromosome length polymorphisms in a Septoria tritici population. Curr Genet 19:265–271
Momol EA, Kistler HC (1992) Mitochondrial plasmids do not determine host range in crucifer-infecting strains of Fusarium oxysporum. Plant Pathol 41:103–112
Morales VM, Séguin-Swartz G, Taylor JL (1993) Chromosome size polymorphism in Leptosphaeria maculans. Phytopathology 83:503–509
Orbach M (1992) One liners. Fungal Genet Newslett 39:92
Ploetz RC (1990) Population biology of Fusarium oxysporum f. sp. cubense. In: Ploetz RC (ed) Fusarium wilt of Banana. APS Press, St. Paul, Minnesota, pp 63–76
Ploetz RC, Correll JC (1988) Vegetative compatibility among races of Fusarium oxysporum f. sp. cubense. Plant Dis 72:325–328
Russell BW, Mills D (1993) Electrophoretic karyotypes of Tilletia caries, T. controversa, and their F1 progeny: further evidence of conspecific status. Mol Plant-Microbe Interact 6:66–74
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Selker EU (1990) Premeiotic instability of repeated sequences in Neurospora crassa. Annu Rev Genet 24:579–613
Author information
Authors and Affiliations
Additional information
Communicated by O. C. Yoder
Rights and permissions
About this article
Cite this article
Kistler, H.C., Benny, U., Boehm, E.W.A. et al. Genetic duplication in Fusarium oxysporum . Curr Genet 28, 173–176 (1995). https://doi.org/10.1007/BF00315784
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00315784

