Summary
Morphology of the primary optic rudiment of normal eyed and mutant eyeless (e/e) axolotl embryos was studied at light, TEM and SEM levles. The presumptive eyeforming region of eyeless embryos differs from that of normal embryos in several important respects including premature formation of basal lamina, separation from overlying ectoderm by mesenchyme cells and persistence of granules in the interspace surrounding the optic anlage into relatively late developmental stages. These differences suggest that the gene that causes failure of eye formation in the mutant axolotls produces structural differences that interfer with normal physical and/or biochemical inductive interactions between neurectoderm and mesenchyme cells due primarily to the precocious development of basal lamina over the extermal surface of the optic primordia.
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Bodian, D.: A new method for staining nerve fibers and nerve endings in mounted paraffin sections. Anat. Rec. 65, 89–97 (1936)
Cohen, A.I.: Electron microscopic observations on the developing mouse eye. Basement membrane during early formation and lens formation. Dev. Biol. 3, 296–316 (1961)
Eakin, R.M., Lehmann, F.E.: An electronmicroscopic study of amphibian ectoderm. Roux' Archiv. Entw-mech. 150, 177–198 (1957)
Epp, L.G.: Development of pigmentation in the eyeless mutant of the Mexican axolotl, Ambystoma mexicanum Shaw. J. Exp. Zool. 181, 169–180 (1972)
Humphrey, R.R.: A recently discovered mutant “eyeless” in the Mexican axolotl Ambystoma mexicanum. Anat. Rec. 163, 206A (1969)
Kalt, M.R., Tandler, B.: A study of fixation of early amphibian embryos for electron microscopy. J. Ultrastruc. Res. 36, 633–645 (1971)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A (1965)
Kelley, R.C.: An electron microscopic study of chrodamesoderm-neurectoderm association in gastrulae of a toad, Xenopus laevis. J. Exp. Zool. 172, 153–180 (1969)
Lofberg, J.: Apical surface topography of invaginating and non-invaginating cells. A scanning-transmission study of amphibian neurulae. Dev. Biol. 36, 311–339 (1974)
Lopashov, G.V., Stroeva, O.G.: Development of the Eye. New York: Daniel Davey & Co., 1964
Schreckenberg, G.M., Jacobson, A.G.: Normal stages of development of the axolotl Ambystoma mexicanum. Dev. Biol. 42, 391–400 (1975)
Schroeder, T.E.: Neurulation in Xenopus laevis. An analysis and model based upon light and electron microscopy. J. Embryol. Exp. Morph. 23, 427–462 (1970)
Snyder, H.D.: Morphology of the hypothalamus of the normal and mutant eyeless axolotl, Ambystoma mexicanum. M.S. Thesis, University Park, Pa. (1975)
Spurr, A.R.: A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruc. Res. 26, 31–43 (1969)
Tarin, D.: Ultrastructural features of neural induction in Xenopus laevis. J. Anat. 111, 1–28 (1972)
Takaya, H., Watanabe, T.: Differential proliferation of the ependyma in the developing neural tube of amphibian embryo. Embryologia 6, 169–176 (1961)
Ulshafer, R.J., Hibbard, E.: Morphology of the optic rudiment in eyed and eyeless axolotls. Anat. Rec. 184, 552A (1976)
Van Deusen, E.: Experimental studies on a mutant gene (e) preventing the differentiation of eye and normal hypothalamus primordia in the axolot. Dev. Biol. 34, 135–158 (1973)
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Ulshafer, R.J., Hibbard, E. An SEM and TEM study of suppression of eye development in eyeless mutant axolotls. Anat Embryol 156, 29–35 (1979). https://doi.org/10.1007/BF00315713
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DOI: https://doi.org/10.1007/BF00315713