Summary
The guinea pig embryo has been grown from the blastocyst to the egg cylinder stage in vitro. Moreover, histological, cytological and cytophotometrical studies have shown that the in vitro-derived egg cylinders closely resemble age-matched, in vivo embryos. In addition, constituent tissue layers were also isolated from the in vivo and the in vitro-derived egg cylinders. These were subsequently grown in culture and found to be, upon cytophotometrical study, similar in nuclear DNA content. Results thus obtained further support the idea that morphogenesis in culture paralleled normal development.
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References
Amoroso EC (1952) Placentation. In: Parkes AS (ed), Mashall: Physiology of reproduction, Vol II, Longmans Press, London, p 127–131
Bernstein EG, Hooper ML, Grandechamp S, Ephrussi B (1973) Alkaline phosphatase activity in mouse teratomas. Proc Natl Acad Sci USA 70:3899–3903
Blandau RJ (1949) Observations on the implantation of the guinea pig ovum. Anat Rec 103:19–47
Blandau RJ (1971) Culture of the guinea pig blastocyst. In: Blandau RJ (ed.) The Biology of the blastocyst, Univ of Chicago Press, Chicago, pp 59–69
Borek C (1975) Studies on normal and neoplastic liver cells in culture: contact behaviour cellular communication, and transformation, In: Gerschenson LE, Thompson EC (eds). Gene expression and carcinogenesis in cultured liver, Acad Press, New York, p 62
Boyd JD, Hamilton WJ (1970) The Human placenta, Heffer, W & Sons, Cambridge
Cole RJ, Paul J (1965) Properties of cultured preimplantation mouse and rabbit embryos and the cell strains derived from them. In: Wolstenholme GE, O'Connor M (eds) Preimplantation stages of pregnancy. J & A Churchill, London, pp 82–112
Cole RJ, Edwards RG, Paul J (1965) Cytodifferentiation in cell colonies and cell strains derived from cleaving ova and blastocysts of the rabbit. Exp Cell Res 37:501–504
Copp AJ (1978a) Interaction between inner cell mass and trophectoderm of the mouse. I. A study of cellular proliferation. J Embryol Exp Morphol 48:109–125
Copp AJ (1979) Interaction between inner cell mass and trophectoderm of the mouse. II. The fate of polar trophectoderm. J Embryol Exp Morphol 51:109–120
Copp AJ (1980) The development of field vole (Microtus agrestis) and mouse blastocysts in vitro: A study of trophoblast cell migration. Placenta 1: 47–60
Copp AJ (1981) The mechanism of mouse egg cylinder morphogenesis in vitro. J Embryol Exp Morphol 61:277–287
Daniel JC (1971) Culture of the rabbit blastocyst across the implantation period. In: Daniel JC (ed). Methods in mammalian embryology, Freeman, WH and Co San Francisco, pp 284–289
Festing MFW (1976) Genetics. In: Mannings PJ, Wagner JE. Biology of the guinea pig. Acad Press New York, p 116, Table IV, Section IV
Gardner RL (1972) An investigation of inner cell mass and trophoblastic tissue following their isolation from the mouse blastocyst. J Embryol Exp Morphol 28:279–312
Gardner RL, Papaioannou VE, Barton SC (1973) Origin of the ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass. J Embryol Exp Morphol 30:561–572
Hogan B, Tilly R (1977) In vitro culture and differentiation of normal mouse blastocysts. Nature 265:626–629
Hogan B, Tilly R (1978) In vitro development of inner cell masses isolated from mouse blastocysts incubated for 24 h before immunosurgery. J Embryol Exp Morphol 45:93–105
Hsu YC (1972) Differentiation in vitro of mouse embryos beyond the implantation stage. Nature 239:200–202
Hsu YC (1978) In vitro development of whole mouse embryos beyond the implantation stage. In: Daniel JC (ed). Methods in mammalian reproduction, Acad Press, New York, pp 229–245
Hsu YC (1979) In vitro development of individually cultured whole mouse embryos from blastocyst to early somite stage. Dev Biol 68:453–469
Ilgren EB (1980a) The control of trophoblastic growth in the guinea pig. J Embryol Exp Morphol 60:405–418
Ilgren EB (1980b) Placental polyploidization: A comparative study. Placenta (In press)
Ilgren EB (1980c) Polyploidization of extraembryonic tissues during mouse embryogenesis. J Embryol Exp Morphol 59:103–111
Kaufman P, Davidoff M (1977) The guinea pig placenta. In: Advances in anatomy, embryology and cell biology. Vol 53. Springer-Verlag, Berlin
Martin GR and Evans KJ (1976) Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc Natl Acad Sci 72:1444–1447
McLaren A, Hensleigh HC (1975) Culture of mammalian embryos over the implantation period. In: Balls M, Wild AE (eds) The early development of mammals. Second British Society for Developmental Biology, Symposium, Cambridge University Press, London, pp 45–60
New DAT (1978) Whole embryo culture and the study of mammalian embryos during organogenesis. Bio Rev 53:81–122
Ptyler R, and Strasser H (1925) Die Vorgänge in Meerschweinchenmuterus von der Inokulation des Eis bis zur Bildung des Placentadiskus. Z f Anat u Entwick, Bd 76:386–420
Rossant J, Offer L (1977) Properties of extraembryonic ectoderm isolated from postimplantation mouse embryos. J Embryl Exp Morphol: 183–194
Samson G, Hill J (1931) Observations on the structure and mode of implantation of the blastocyst of Cavia. Trans Soc Zool London 21:295–354
Sherman MI (1975a) Long term culture of cells derived from mouse blastocysts. Differentiation 3:51–67
Sherman MI (1975b) The culture of cells derived from mouse blastocyts. Cell 5:343–349
Sherman MI (1978) Implantation of mouse blastocysts in vitro. In: Daniel JD, Methods in mammalian reproduction. Acad Press, New York, pp 247–257
Sherman MI, Wudl LR (1976) The implanting mouse blastocyst. In: Poste G, Nicholson G (eds) The cell surface in animal embryogenesis and development, N Holland-Elsevier, Amsterdam, pp 81–125
Snedecor GW, Cochran WE (1976) Statistical methods, 6th eds, (formulae 8.10.3). Iowa State Univ Press, p 217
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Ilgren, E.B. The in vitro morphogenesis of the guinea pig egg cylinder. Anat Embryol 163, 351–365 (1981). https://doi.org/10.1007/BF00315711
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DOI: https://doi.org/10.1007/BF00315711