Summary
The distribution of horseradish peroxidase (HRP) in intraarterial cushions at the origins of intercostal arteries was studied in mice 30 min after intravenous injection of the tracer.
HRP is found to be evenly distributed in the extracellular space of the entire arterial wall. Basal lamina material is penetrated by the tracer protein. This is not the case with collagenic fibrils, elastic material and intercellular granular deposits which occur regularly in cushions of adult mice but not in those of newborn animals (granules 300 to 800 Å in diameter, probably cell debris). However, the elastic sheets do not act as diffusion barriers. HRP is not phagocytized in significant amounts by ramified smooth muscle cells within the time intervals tested. It is therefore assumed that granular deposits are also not phagocytized by smooth muscle cells.
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References
Böck, P.: Das Glomus caroticum der Maus. Advanc. Anat. Embryol. 48, 1–84 (1973)
Burnstock, G.: Innervation of vascular smooth muscle: histochemistry, and electron, microscopy. Clin. exp. Pharmacol. Physiol., Suppl. 2, 7–20 (1975)
Giacomelli, F., Juechter, K. B., Wiener, J.: The cellular pathology of experimental hypertension. VI. Alterations in retinal vasculature. Amer. J. Path. 168, 81–88 (1972)
Giacomelli, F., Wiener, J.: Regional variation in the permeability of rat thoracic aorta. Amer. J. Path. 75, 513–528 (1974)
Gorgas, K., Böck, P.: Studies on intra-arterial cushions. I. Morphology of the cushions at the origins of intercostal arteries in mice. Anat. Embryol. 148, 59–72 (1975)
Graham, R. C., Jr., Karnovsky, M. J.: The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem. 14, 291–302 (1966)
Joris, I., Majno, G.: Cellular breakdown within arterial wall. An ultrastructural study of the coronary artery in young and aging rats. Virchows Arch. Abt A 364, 111–127 (1974)
Kojimahara, M., Ooneda, G.: Electron microscopic study on the middle cerebral artery lesions in hypertensive rats. Acta path. jap. 20, 399–408 (1970)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)
Stein, O., Stein, Y.: An electron microscopic study of the transport of peroxidase in the endothelium of mouse aorta. Z. Zellforsch. 133, 211–222 (1972)
Steinmann R. N., Cohn, Z. A.: The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro. J. Cell Biol. 55, 186–204 (1972)
Wiener, J., Giacomelli, F.: The cellular pathology of experimental hypertension. VII. Structure and permeability of the mesenteric vasculature in angiotensin-induced, hypertension. Amer. J. Path. 72, 221–232 (1973)
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Gorgas, K., Böck, P. Studies on intra-arterial cushions. Anat. Embryol. 149, 315–321 (1976). https://doi.org/10.1007/BF00315447
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DOI: https://doi.org/10.1007/BF00315447