Summary
We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30°C but not at 42°C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bainbridge BW, Valentine BP, Markham P (1979) In: Burnett JH, Trinci APJ (eds) Fungal walls and hyphal growth. Cambridge University Press, pp 71–91
Fromtling RA (1988) Drug News Perspectives 1:284–288
Hartwell LH (1967) J Bacteriol 93:1662–1670
Hilton C, Markie D, Corner B, Rikkering E, Poulter R (1985) Mol Gen Genet 200:162–168
Kakar SN, Partridge RM, Magee PT (1983) Genetics 104:241–255
Kurtz MB, Kirsch DR, Kelly R (1988) Microbiol Sci 5:58–63
Poulter RTM, Rikkerink EHA (1983) J Bacteriol 156:1066–1077
Schekman R (1982) Trends Biochem Sci 7:243–246
Sherman F, Fink G, Hicks J (1981) Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Whelan WL, Magee PT (1981) J Bacteriol 145:896–903
Whelan WL, Soll DRE (1982) Mol Gen Genet 187:477–485
Author information
Authors and Affiliations
Additional information
Communicated by B. S. Cox
Rights and permissions
About this article
Cite this article
Payton, M.A., de Tiani, M. The isolation of osmotic-remedial conditional lethal mutants of Candida albicans . Curr Genet 17, 293–296 (1990). https://doi.org/10.1007/BF00314875
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00314875