Abstract
Trophoblast cells were cultured on microporous membrane filters. After incubation at different times with gold-conjugated ligands, the cells were processed for electron microscopy. Gold particles indicating the presence of both IgG and LDL appeared in a time-dependent manner in coated pits and coated vesicles. LDL-gold appeared primarily within lysosomes whereas approximately 50% of the internalized IgG-gold appeared within vesicles (diameters ranging from 35 to 80 nm) near the basal regions of the cell. These vesicles may be the protective mechanism which prevents IgG breakdown during transcytosis across trophoblast cells, thus allowing transport of the intact molecule to the fetus.
Similar content being viewed by others
References
Abrahamson DR, Rodewald R (1981) Evidence for the sorting of endocytic vesicle contents during the receptor-mediated transport of IgG across the newborn rat intestine. J Cell Biol 91:270–280
Contractor SF, Sooranna SR (1986) Monoclonal antibodies to cytotrophoblast and syncytiotrophoblast of human placenta. J Dev Physiol 8:277–282
Contractor SF, Eaton BM, Stannard PJ (1983) Uptake and fate of exogenous immunoglobulin-G in the perfused human placenta. J Reprod Immunol 5:265–273
Geuze HJ, Slot JW, Strous GJAM, Lodish HF, Schwartz AL (1983) Intracellular site of asialoglycoprotein receptor-ligand uncoupling: double-label immunoelectron microscopy during receptor-mediated endocytosis. Cell 32:277–287
Geuze HJ, Slot JW, Strous GJAM, Peppard J, von Figura K, Hasilik A, Schwartz AL (1984) Intracellular receptor sorting during endocytosis. Comparative immunoelectron microscopy of multiple receptors in rat liver. Cell 37:195–204
Goldstein JL, Brown MS, Anderson RGW, Russell DW, Schneider WJ (1985) Receptor-mediated endocytosis: concepts emerging from the receptor system. Ann Rev Cell Biol 1:1–39
Gruenberg J, Griffiths G, Howell KE (1989) Characterization of the early endosome and putative endocytic carrier vesicles in vivo and an assay of vesicle fusion in vitro. J Cell Biol 108:1301–1316
Hopkins CR (1983) The importance of the endosome in intracellular traffic. Nature 304:684–685
Hudson L, Hay FC (1976) Practical Immunology. Blackwell, Oxford
Hunziker W, Harter C, Matter K, Mellman I (1991) Basolateral sorting in MDCK cells requires a distinct cytoplasmic domain determinant. Cell 66:907–920
King BF (1982a) The role of coated vesicles in selective transfer across yolk sac epithelium. J Ultrastruct Res Mol Struct 79:273–284
King BF (1982b) Absorption of peroxidase-conjugated immunoglobulin G by human placenta: an in vitro study. Placenta 3:395–406
Kliman HJ, Nestler JE, Sermasi E, Sanger JM, Strauss III JF (1986) Purification, characterization, and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinology 118:1567–1582
Larkin JM, Palade GE (1991) Transcytotic vesicular carriers for polymeric receptors accumulate in rat hepatocytes after bile duct ligation. J Cell Sci 98:205–216
Leach L, Eaton BM, Firth JA, Contractor SF (1990) Uptake and intracellular routing of peroxidase-conjugated immunoglobulin-G by the perfused human placenta. Cell Tissue Res 261:383–388
Malassiné A, Besse C, Roche A, Alsat E, Rebourcet R, Mondon F, Cedard L (1987) Ultrastructural visualization of the internalization of low density lipoprotein by human placental cells. Histochemistry 87:457–464
Matre R, Tonder O, Enderssen C (1975) Fc receptors in human placenta. Scand J Immunol 4:741–745
Mostov KE, Simister NE (1985) Transcytosis. Cell 43:389–390
Musil LS, Baenziger JU (1987) Cleavage of membrane component to soluble secretory component occurs on the cell surface of rat hepatocyte monolayers. J Cell Biol 104:1725–1733
Pearse BMF (1982) Coated vesicles from human placenta carry ferritin, transferrin and immunoglobulin G. Proc Natl Acad Sci USA 79:451–455
Slot JW, Gueze HJ (1985) A new method for preparing gold probes for multiple-labelling cytochemistry. Eur J Cell Biol 38:87–93
Sooranna SR, Contractor SF (1991) Vectorial transcytosis of immunoglobulin G by human trophoblast cells in culture. Exp Cell Res 192:41–45
Sztul E, Kaplin A, Saucan L, Palade G (1991) Protein traffic between distinct plasma membrane domains: isolation and characterization of vesicular carriers involved in transcytosis. Cell 64:81–89
Van der Meulen JA, McNabb TC, Haeffner-Cavaillon N, Klein M, Dorrington KJ (1980) The Fc receptor on human placenta plasma membrane. I. Studies on the binding of homologous and heterologous immunoglobulin G. J Immunol 124:500–507
Winkel CA, Gilmore I, McDonald PC, Simpson E (1980) Uptake and degradation of lipoproteins by human trophoblast cells in primary culture. Endocrinology 107:1892–1898
Wood G, Reynard J, Krishman E, Racela L (1978) Immunobiology of the human placenta. I. IgG Fc receptors in trophoblastic villi.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sooranna, S.R., Moss, J. & Contractor, S.F. Comparison of the intracellular pathways of immunoglobulin-G and low density lipoprotein in cultured human term trophoblast cells. Cell Tissue Res 274, 619–625 (1993). https://doi.org/10.1007/BF00314560
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00314560