Abstract
Two Phycomyces genes, madI and madJ, which are involved in phototropism, were characterized by recombination and complementation analyses. The madI gene was located on linkage group IV of the genetic map of Phycomyces, 27 map units away from the gene carA. Complementation and recombination studies involving the genes madD, madE, madF, and madG, in combination with previous genetic studies, show that the recently isolated mad-407 mutation defines a novel behavioural gene, madJ, of Phycomyces. A regulatory role of the madJ gene product in the light-sensory transduction pathway is suggested.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alvarez MI, Ootaki T, Eslava AP (1983) Mutants of Phycomyces with abnormal phototropism induced by ICR-170. Mol Gen Genet 191:507–511
Alvarez MI, Eslava AP, Lipson ED (1989) Phototropism mutants of Phycomyces blakesleeanus isolated at low light intensity. Exp Mycol 13:38–48
Arst HN, Scazzocchio C (1985) Formal genetics and molecular biology of the control gene expression in Aspergillus nidulans. In: Bennett JW, Lasure LL (eds) Gene manipulation in fungi. Academic Press, New York, pp 309–343
Bergman K, Burke PW, Cerdá-Olmedo E, David CN, Delbrück M, Foster KW, Goodell EW, Heisenberg M, Meissner G, Zalokar M, Dennison DS, Shoropshire W Jr (1969) Phycomyces. Bacteriol Rev 3:99–157
Bergman K, Eslava AP, Cerdá-Olmedo E (1973) Mutants of Phycomyces with abnormal phototropism. Mol Gen Genet 123:1–16
Campuzano V, Díaz-Minguez JM, Eslava AP, Alvarez MI (1990) A new gene (madI) involved in the phototropic response of Phycomyces. Mol Gen Genet 223:148–151
Campuzano V, Galland P, Senger H, Alvarez MI, Eslava AP (1994) Isolation and characterization of phototropism mutants of Phycomyces insensitive to ultraviolet light. Curr Genet 26:49–53
Cerdá-Olmedo E (1975) The genetics of Phycomyces blakesleeanus. Genet Res 25:285–296
Eslava AP (1987) Genetics. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 27–48
Eslava AP, Alvarez MI, Burke PV, Delbrück M (1975 a) Genetic recombination between mutants of Phycomyces. Genetics 80:445–462
Eslava AP, Alvarez MI, Delbrück M (1975b) Meiosis in Phycomyces. Proc Natl Acad Sci USA 72:4076–4080
Gauger W, Pelaez MI, Alvarez MI, Eslava AP (1980) Mating-type heterokaryons in Phycomyces blakesleeanus. Exp Mycol 4:56–64
Heisenberg M, Cerdá-Olmedo E (1968) Segregation of heterokaryons in the asexual cycle of Phycomyces. Mol Gen Genet 102: 187–195
Lipson ED, López-Díaz I, Pollock JA (1983) Mutants of Phycomyces with enhanced tropisms. Exp Mycol 7:241–252
López-Díaz I, Lipson ED (1983) Meiotic dysgenesis associated with behavioural mutants of Phycomyces. Curr Genet 7:313–322
Orejas M, Peláez MI, Alvarez MI, Eslava AP (1987) A genetic map of Phycomyces blakesleeanus. Mol Gen Genet 210:69–76
Ootaki T (1973) A new method for heterokaryon formation in Phycomyces Mol. Gen Genet 121:49–56
Russo VEA (1977) Ethylene-induced growth in Phycomyces mutants abnormal for autochemotropism. J Bacteriol 130:548–551
Russo VEA, Halloran B, Gallori E (1977) Ethylene is involved in the autochemotropism of Phycomyces. Planta 134:61–67
Scazzocchio C, Arst HN (1978) The nature of an initiator constitutive mutation in Aspergillus nidulans. Nature 274:177–179
Sutter RP (1975) Mutations affecting sexual development in Phycomyces blakesleeanus. Proc Natl Acad Sci USA 72:127–130
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Campuzano, V., Galland, P., Eslava, A.P. et al. Genetic characterization of two phototropism mutants of Phycomyces with defects in the genes madl and madJ . Curr Genet 27, 524–527 (1995). https://doi.org/10.1007/BF00314442
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00314442