Summary
One hundred and twenty samples of haemorrhagic spinal fluid were examined by acid phosphatase staining. This enzyme activity starts to appear in mono-histiocytic cells 2 days after bleeding and increases up to the 5th day. After 1 week the activity decreases rapidly. Similar results are found in mixtures of incubated clear spinal fluid, to which blood is added. Acid phosphatase staining is a useful additional method for determination of the age of a leptomeningeal bleed.
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References
Anderson PJ, Song SK (1962) Acid phosphatase in the nervous system. J Neuropathol Exp Neurol 62:263–283
Cardoza PL (1958) Advantages and disadvantages of the May-Grünwald Giemsa procedure in exfoliative cytology. Acta Cytol (Baltimore) 2:284
De Reuck J, De Coster W, vander Eecken H (1984) Acid phosphatase activity of cerebrospinal fluid cells in multiple sclerosis and related neurological disorders. In: Gonsette RE, Delmotte P (eds) Immunological and clinical aspects of multiple sclerosis. MTP Press, Lancaster Boston The Hague Dordrecht, pp 83–87
Dufresne JJ (1972) Cytologie pratique du liquide céphalo-rachidien. Ciba-Geigy, Basle, pp 39–45
Engelhardt P (1975) Diagnostischer Wert der Siderophagen im Liquor Cytogramm. J Neurol 208:201–206
Gomori G (1950) An improved histochemical technic for acid phosphatase. Stain Technol 25:81
Kölmer HW (1977) Atlas of cerebrospinal fluid cells, 2nd edn. Springer-Verlag, Berlin Heidelberg New York, pp 20–33
Novikoff A (1961) Lysosomes and related particles. In: Brachet J, Mirsky A (eds) The cell. Biochemistry, physiology, morphology, vol II. Academic Press, New York, pp 423–488
Oehmichen M, Schütze G (1973) Erythrophagen in der Liquorzelldiagnostik der Subarachnoidalblutung. Nervenarzt 44:407–416
Olischer RM (1971) Der Nachweis der unspezifischen Esterase in Liquorzellen. Eine cytodiagnostische Zusatzuntersuchung. J Neurol 200:61–69
Olischer RM (1972) Die PAS Reaktion in der Zytodiagnostik des Liquor cerebrospinalis. Psychiatr Neurol Med Psychol (Leipz) 24:716–726
Peiffer J, Schwarze EW (1971) Beitrag zur Enzymhistochemie der Liquor- und Leptomeningeal Zellen. Nervenarzt 42:267–269
Quincke H (1891) Die Lumbalpunction des Hydrocephalus. Klin Wochenschr 28:929–932
Vahar-Matiar H, Müller J, Lippes G (1973) Der blutige Liquor. Acta Neurochir (Wien) 29:229–245
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de Reuck, J., de Coster, W. & Vander Eecken, H. Acid phosphatase activity of cerebrospinal fluid cells in leptomeningeal haemorrhage. J Neurol 232, 154–156 (1985). https://doi.org/10.1007/BF00313891
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DOI: https://doi.org/10.1007/BF00313891