Abstract
Mitochondrial DNA (mtDNA) from the European seabass, Dicentrarchus labrax, has been cloned and characterized. Its gene organization was deduced by a comparison of the sequenced termini of different subclones obtained from European seabass mtDNA to the completely-sequenced mtDNAs from carp and freshwater loach. The difference in genome size between the European seabass mtDNA (approximately 18 kb) and most of the other characterized fish mtDNAs (approximately 16.5 kb) is accounted for by the displacement-loop (D-loop). Comparisons have been performed between the derived amino-acid sequences of three sequenced genes, cytochrome c oxidase subunit 2 (COII), NADH dehydrogenase subunit 4L (ND4L) and ATP synthase subunit 8 (ATPase8), from D. labrax, and their counterparts in other fishes and Xenopus laevis.
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Allegrucci G, Fortunato C, Cataudella S, Sbordoni V (1994) In: Beaumont AR (ed) Genetics and evolution of aquatic organisms. Chapman and Hall, London, pp 486–502
Anderson S, Bankier AT, Barrel BG, DeBruijn MHL, Coulson AR, Drouin J, Eperon IE, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465
Anderson S, DeBruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156:683–717
Årnason U, Johnsson E (1992) J Mol Evol 34:493–505
Årnason U, Gullberg A, Widegrenn B (1991) J Mol Evol 33:556–568
Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26:167–180
Billington N, Hebert PDN (1991) Can J Fish Aquat Sci 48:80–94
Cecconi F, Venanzetti F, Mariottini P (1993) Nucleic Acids Res 21: 2253
Chomyn A, Mariottini P, Cleeter MWJ, Ragan CI, Doolittle RF, Yagi AM, Hatefi Y, Attardi G (1985) Functional assignment of the products of the unidentified reading frames of human mitochondrial DNA. In: Quagliariello E, Slater EC, Palmieri F, Saccone C, Kroon AM (eds) Achievements and perspectives of mitochondrial research, vol. 2. Biogenesis. Elsevier Science Publishers, Amsterdam, pp 259–275
Davidson WS, Birt TP, Green JM (1989) Genome 32:340–342
Desjardins P, Morais R (1990) J Mol Biol 212:599–634
Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13
Felsenstein J (1989) Cladistics 5:164–166
French S, Robson B (1983) J Mol Evol 19:171–175
Gadaleta G, Pepe G, De Candia G, Quagliariello C, Sbisa E, Saccone C (1989) J Mol Evol 28:497–516
Gilbert TL, Brown JR, O'Hara PJ, Buroker NE, Beckenbach AT, Smith MJ (1988) Nucleic Acids Res 24:11825
Gjetvaj B, Cook DI, Zouros E (1992) Mol Biol Evol 9:106–124
Gonzalez-Villaseñor LI, Burkhoff AM, Corces V, Powers DA (1986) Can J Fish Aquat Sci 43:1866–1872
Grachev MA, Slobodyanyuk SJa, Kholodilov NG, Fyodorov SP, Belikov SI, Sherbakov DYu, Sideleva VG, Zubin AA, Kharchenkov VV (1992) J Mol Evol 34:85–90
Huang FL (1991) EMBL Data Library X61010
Johansen S, Guddal PH, Johansen T (1990) Nucleic Acids Res 18: 411–419
Lansman RA, Shade RO, Shapira JF, Avise JC (1981) J Mol Evol 17:214–226
Meyer A, Kocher TD, Basasibwaki P, Wilson AC (1990) Nature 347: 550–553
Meyer A (1994) Evolution of mitochondrial DNA in fishes. In: Hochachka PW, Mommsen TP (eds) The biochemistry and molecular biology of fishes, vol. 2. Elsevier Science Publishers, Amsterdam (in press)
Prichard AE, Seilhamer JJ, Mahalingam R, Sable CL, Venuti SE, Cummings DJ (1990) Nucleic Acids Res 18:173–180
Roe BA, Ma DP, Wilson RK, Wong JFH (1985) J Biol Chem 260: 9759–9774
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sturmbauer C, Meyer A (1992) Nature 358:578–581
Thomas WK, Beckenbach AT (1989) J Mol Evol 29:233–245
Tzeng C-S, Hui C-F, Shen S-C, Huang PC (1992) Nucleic Acids Res 18:4853–4858
Wallace DC (1982) Microbiol Rev 46:208–240
Warrior R, Gall J (1985) Arch Sci Geneve 3:439–445
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Venanzetti, F., Cecconi, F., Giorgi, M. et al. Cloning and characterization of the European seabass, Dicentrarchus labrax, mitochondrial genome. Curr Genet 26, 139–145 (1994). https://doi.org/10.1007/BF00313802
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DOI: https://doi.org/10.1007/BF00313802