Skip to main content
Log in

Cloning and characterization of the European seabass, Dicentrarchus labrax, mitochondrial genome

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Abstract

Mitochondrial DNA (mtDNA) from the European seabass, Dicentrarchus labrax, has been cloned and characterized. Its gene organization was deduced by a comparison of the sequenced termini of different subclones obtained from European seabass mtDNA to the completely-sequenced mtDNAs from carp and freshwater loach. The difference in genome size between the European seabass mtDNA (approximately 18 kb) and most of the other characterized fish mtDNAs (approximately 16.5 kb) is accounted for by the displacement-loop (D-loop). Comparisons have been performed between the derived amino-acid sequences of three sequenced genes, cytochrome c oxidase subunit 2 (COII), NADH dehydrogenase subunit 4L (ND4L) and ATP synthase subunit 8 (ATPase8), from D. labrax, and their counterparts in other fishes and Xenopus laevis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Allegrucci G, Fortunato C, Cataudella S, Sbordoni V (1994) In: Beaumont AR (ed) Genetics and evolution of aquatic organisms. Chapman and Hall, London, pp 486–502

    Google Scholar 

  • Anderson S, Bankier AT, Barrel BG, DeBruijn MHL, Coulson AR, Drouin J, Eperon IE, Nierlich DP, Roe BA, Sanger F, Schreier PH, Smith AJH, Staden R, Young IG (1981) Nature 290:457–465

    Google Scholar 

  • Anderson S, DeBruijn MHL, Coulson AR, Eperon IC, Sanger F, Young IG (1982) J Mol Biol 156:683–717

    Google Scholar 

  • Årnason U, Johnsson E (1992) J Mol Evol 34:493–505

    Google Scholar 

  • Årnason U, Gullberg A, Widegrenn B (1991) J Mol Evol 33:556–568

    Google Scholar 

  • Bibb MJ, Van Etten RA, Wright CT, Walberg MW, Clayton DA (1981) Cell 26:167–180

    Google Scholar 

  • Billington N, Hebert PDN (1991) Can J Fish Aquat Sci 48:80–94

    Google Scholar 

  • Cecconi F, Venanzetti F, Mariottini P (1993) Nucleic Acids Res 21: 2253

    Google Scholar 

  • Chomyn A, Mariottini P, Cleeter MWJ, Ragan CI, Doolittle RF, Yagi AM, Hatefi Y, Attardi G (1985) Functional assignment of the products of the unidentified reading frames of human mitochondrial DNA. In: Quagliariello E, Slater EC, Palmieri F, Saccone C, Kroon AM (eds) Achievements and perspectives of mitochondrial research, vol. 2. Biogenesis. Elsevier Science Publishers, Amsterdam, pp 259–275

    Google Scholar 

  • Davidson WS, Birt TP, Green JM (1989) Genome 32:340–342

    Google Scholar 

  • Desjardins P, Morais R (1990) J Mol Biol 212:599–634

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13

    Google Scholar 

  • Felsenstein J (1989) Cladistics 5:164–166

    Google Scholar 

  • French S, Robson B (1983) J Mol Evol 19:171–175

    Google Scholar 

  • Gadaleta G, Pepe G, De Candia G, Quagliariello C, Sbisa E, Saccone C (1989) J Mol Evol 28:497–516

    Google Scholar 

  • Gilbert TL, Brown JR, O'Hara PJ, Buroker NE, Beckenbach AT, Smith MJ (1988) Nucleic Acids Res 24:11825

    Google Scholar 

  • Gjetvaj B, Cook DI, Zouros E (1992) Mol Biol Evol 9:106–124

    Google Scholar 

  • Gonzalez-Villaseñor LI, Burkhoff AM, Corces V, Powers DA (1986) Can J Fish Aquat Sci 43:1866–1872

    Google Scholar 

  • Grachev MA, Slobodyanyuk SJa, Kholodilov NG, Fyodorov SP, Belikov SI, Sherbakov DYu, Sideleva VG, Zubin AA, Kharchenkov VV (1992) J Mol Evol 34:85–90

    Google Scholar 

  • Huang FL (1991) EMBL Data Library X61010

  • Johansen S, Guddal PH, Johansen T (1990) Nucleic Acids Res 18: 411–419

    Google Scholar 

  • Lansman RA, Shade RO, Shapira JF, Avise JC (1981) J Mol Evol 17:214–226

    Google Scholar 

  • Meyer A, Kocher TD, Basasibwaki P, Wilson AC (1990) Nature 347: 550–553

    Google Scholar 

  • Meyer A (1994) Evolution of mitochondrial DNA in fishes. In: Hochachka PW, Mommsen TP (eds) The biochemistry and molecular biology of fishes, vol. 2. Elsevier Science Publishers, Amsterdam (in press)

    Google Scholar 

  • Prichard AE, Seilhamer JJ, Mahalingam R, Sable CL, Venuti SE, Cummings DJ (1990) Nucleic Acids Res 18:173–180

    Google Scholar 

  • Roe BA, Ma DP, Wilson RK, Wong JFH (1985) J Biol Chem 260: 9759–9774

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Sturmbauer C, Meyer A (1992) Nature 358:578–581

    Google Scholar 

  • Thomas WK, Beckenbach AT (1989) J Mol Evol 29:233–245

    Google Scholar 

  • Tzeng C-S, Hui C-F, Shen S-C, Huang PC (1992) Nucleic Acids Res 18:4853–4858

    Google Scholar 

  • Wallace DC (1982) Microbiol Rev 46:208–240

    Google Scholar 

  • Warrior R, Gall J (1985) Arch Sci Geneve 3:439–445

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Venanzetti, F., Cecconi, F., Giorgi, M. et al. Cloning and characterization of the European seabass, Dicentrarchus labrax, mitochondrial genome. Curr Genet 26, 139–145 (1994). https://doi.org/10.1007/BF00313802

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00313802

Key words

Navigation