Summary
Animal models allow determination of tumor response to anticancer agents under various experimental conditions. The chemically induced 9L rat brain tumor has been developed as both in vivo and in vitro models. Animal survival, clonogenic cell survival, and tumor growth delay provide means to measure the effectiveness of treatment modalities in this tumor model. Monolayer cultures, multicellular spheroid cultures, brain tumors, and flank tumors have been used to study the influence of different biological entities of the 9L model on the response to treatment with radiation and/or BCNU (1,3- bis (2-chloroethyl)-1-nitrosourea).
Zusammenfassung
Die Behandlung von Tumoren unterliegt den Einflüssen verschiedener Umgebungsfaktoren, die an Tiertumormodellen erforscht werden konnten. Für den chemisch induzierten 9L Hirntumor der Ratte wurden in vivo- and in vitro-Systeme entwickelt. Überlebensraten von Tieren oder clonogenen Zellen und Wachstumsverzögerung von Tumoren können gemessen werden, um die Wirksamkeit verschiedener Tumorbehandlungen zu bestimmen. Monolayer- und Spheroidkulturen sowie intracerebral oder subcutan wachsende Tumoren wurden verwendet, um den Einfluß unterschiedlicher Tumorgestalt auf die Wirkung von Strahlen und/oder BCNU (1,3- bis (2-chloroethyl)-1-nitrosourea) bei 9L Zellen zu demonstrieren.
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Supported by NIH Program Project Grant CA-13525, the Phi Beta Psi Sorority and the Thomas H. Dwyer Memorial Funds, and a grant from the Deutsche Forschungsgemeinschaft
Research Fellow at the Brain Tumor Research Center; permanent address: Department of Neurology, Klinikum Charlottenburg, Free University of Berlin, West Germany
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Weizsaecker, M., Deen, D.F., Rosenblum, M.L. et al. The 9L rat brain tumor: Description and application of an animal model. J Neurol 224, 183–192 (1981). https://doi.org/10.1007/BF00313280
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DOI: https://doi.org/10.1007/BF00313280