Summary
Myelin was isolated from histologically normal white matter and plaques from MS patients and from white matter of neurologically normal controls. No difference was found in the total lipid content. There were no detectable deficits in MS myelin of phosphoglycerides, plasmalogens or sphingolipids. Gangliosides and lysolecithin were not detected. Analysis of the fatty aldehyde composition of the phosphoglycerides and the fatty acid composition of the cholesteryl esters, phosphoglycerides and sphingolipids did not show any differences between the normal and MS myelin.
Zusammenfassung
Myelin wurde aus Plaques von MS-Patienten sowie aus histologisch normaler weißer Substanz des Gehirns von Patienten und von Kontrollfällen isoliert. Es wurde kein Unterschied im Gesamtlipidgehalt festgestellt. Es war kein faßbarer Mangel an Phosphoglyceriden, plasmalogenen und Sphingolipiden im Myelin der MS-Fälle nachweisbar. Ganglioside und Lysolecithine wurden nicht gefunden. Eine Analyse der Zusammensetzung der fetthaltigen Aldehyde der Phosphoglyceride und der Fettsäurezusammensetzung der Cholesterolester, der Phosphoglyceride und der Sphingolipide zeigte keinerlei Unterschiede zwischen dem normalen Myelin und dem Myelin in den MS-Fällen.
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References
Alling, C., Vanier, M. T., Svennerholm, L.: Lipid alterations in apparently normal white matter in multiple sclerosis. Brain Res. 35, 325–326 (1971)
Arnetoli, G., Pazzagli, A., Amaducci, L.: Fatty acid and aldehyde changes in cholineand ethanolamine-containing phospholipids in the white matter of multiple sclerosis brains. J. Neurochem. 16, 461–463 (1969)
Baker, R. W. R., Thompson, R. H. S., Zilkha, K. J.: Fatty-acid composition of brain lecithins in multiple sclerosis. Lancet 1963 I, 26–27
Bernsohn, J., Stephanides, L. M.: Aetiology of multiple sclerosis. Nature (Lond.) 215, 821–823 (1967)
Clausen, J., Möller, J.: Allergic encephalomyelitis induced by brain antigen after deficiency in polyunsaturated fatty acid during myelination. Acta neurol. scand. 43, 375–388 (1967)
Clausen, J., Hansen, I. B.: Myelin constituents of human central nervous system. Acta neurol. scand. 46, 1–17 (1970)
Cumings, J. N.: The cerebral lipids in disseminated sclerosis and in amaurotic family idiocy. Brain 76, 551–562 (1953)
Cumings, J. N.: Lipid chemistry of the brain in demyelinating diseases. Brain 78, 554–563 (1955)
Cumings, J. N., Goodwin, H.: Sphingolipids and phospholipids of myelin in multiple sclerosis. Lancet 1968 II, 664–665
Cuzner, M. L., Davison, A. N., Gregson, N. A.: The chemical composition of vertebrate myelin and microsomes. J. Neurochem. 12, 469–481 (1965)
Davison, A. N., Wajda, M.: Cerebral lipids in multiple sclerosis. Neurochem. 9, 427–432 (1962)
Eng, L. F., Chao, F. C., Gerstl, B., Pratt, D., Tavaststjerna, M. G.: The maturation of human white matter myelin. Biochemistry, Easton 7, 4455–4465 (1968)
Fewster, M. E., Mead, J. F.: Lipid composition of glial cells isolated from bovine white matter. J. Neurochem. 15, 1041–1052 (1968)
Fewster, M. E., Mead, J. F.: Fatty acid and fatty aldehyde composition of glial cell lipids isolated from bovine white matter. J. Neurochem. 15, 1303–1312 (1968)
Fewster, M. E., Burns, B. J., Mead, J. F.: Quantitative densitometric thin-layer chromatography of lipids using copper acetate reagent. J. Chromatog. 43, 120–126 (1969)
Fewster, M. E., Mead, J. F., Wolfgram, F. J., Tourtellotte, W. W.: Cholesterol esters in myelin isolated from cerebral white matter of patients with multiple sclerosis. Proc. Soc. exp. Biol. (N.Y.) 133, 795–800 (1970)
Fewster, M. E., Hirono, H., Mead, J. F.: The stability of myelin ethanolamine glycerophosphatide in post-mortem human and bovine brains. J. Neurochem. 19, 1803–1805 (1972)
Gerstl, B., Kahnke, M. J., Smith, J. K., Tavaststjerna, M. G., Hayman, R. B.: Brain lipids in multiple sclerosis and other diseases. Brain 84, 310–19 (1961)
Gerstl, B., Tavaststjerna, M. G., Hayman, R. B., Smith, J. K., Eng, L. F.: Lipid studies of white matter and thalamus of human brains. J. Neurochem. 10, 889–902 (1963)
Gerstl, B., Tavaststjerna, M. G., Hayman, R. B., Eng, L. F., Smith, J. K.: Alterations in myelin fatty acids and plasmalogens in multiple sclerosis. Ann. N.Y. Acad. Sci. 122, 405–416 (1965)
Gerstl, B., Eng, L. F., Hayman, R. B., Tavaststjerna, M. G., Bond, P. R.: On the composition of human myelin. J. Neurochem. 14, 661–670 (1967)
Gerstl, B., Eng, L. F., Tavaststjerna, M. G., Smith, J. K., Kruse, S. L.: Lipids and proteins in multiple sclerosis white matter. J. Neurochem. 17, 677–689 (1970)
Gerstl, B.: The biochemistry of demylination and demylinating diseases. In: Biochemical aspects of nervous diseases (ed. J. N. Cumings), p. 69. London: Plenum Press 1972
Laatsch, R. H., Kies, M. W., Gordon, S., Alvord, E. C.: The encephalomyelitic activity of myelin isolated by ultracentrifugation. J. exp. Med. 115, 777–788 (1962)
Mangold, M. K., Malins, D. C.: Fractionation of fats, oils, and waxes on thin layers of silicic acid. J. Amer. Oil Chem. Soc. 37, 383–385 (1960)
O'Brien, J. S., Sampson, E. L.: Lipid composition of the normal human brain: gray matter, white matter, and myelin. J. Lipid Res. 6, 537–544 (1965)
O'Brien, J. S., Sampson, E. L.: Fatty acid and fatty aldehyde composition of the major brain lipids in normal human gray matter, white matter, and myelin. J. Lipid Res. 6, 545–551 (1965)
Privett, O. S., Blank, M. L., Codding, D. W., Nickell, E. C.: Lipid analysis by quantitative thin-layer chromatography. J. Amer. Oil Chem. Soc. 42, 381–393 (1965)
Rouser, G., Galli, C., Lieber, E., Blank, M. L., Privett, O. S.: Analytical fractionation of complex lipid mixtures: DEAE cellulose column chromatography combined with quantitative thin layer chromatography. J. Amer. Oil Chem. Soc. 41, 836–840 (1964)
Scheinberg, L. C., Korey, S. R.: Multiple sclerosis. Ann. Rev. Med. 13, 411–430 (1962)
Suzuki, K., Kamoshita, S., Eto, Y., Tourtellotte, W. W., Gonatas, J. O.: Myelin in multiple sclerosis. Arch. Neurol. 28, 293–297 (1973)
Thompson, R. H. S.: The biochemistry of multiple sclerosis. The Scientific Basis of Medicine Annual Reviews, pp. 283–301 (1962)
Thompson, R. H. S., Cumings, J. N.: In: Biochemical disorders in human disease (eds. R. H. S. Thompson, E. J. King), p. 69. London: J. and A. Churchill Ltd. 1964
Thompson, R. H. S.: A biochemical approach to the problem of multiple sclerosis. Proc. roy. Soc. Med. 59, 269–276 (1966)
Yanagihara, T., Cumings, J. N.: Alterations of phospholipids, particularly plasmalogens, in the demyelination of multiple sclerosis as compared with that cerebral oedema. Brain 92, 59–70 (1969)
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This investigation was supported by Grant 484 from the National Multiple Sclerosis Society and by USPHS Grants HD 04575, NB 05464, GM-K6-19177.
The postmortem specimens were provided from the International Tissue Bank supported by the National Multiple Sclerosis Society and administered by Wallace W. Tourtellotte, M.D., Ph.D.
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Fewster, M.E., Hirono, H. & Mead, J.F. Lipid composition of myelin in multiple sclerosis. J. Neurol. 213, 119–131 (1976). https://doi.org/10.1007/BF00313273
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DOI: https://doi.org/10.1007/BF00313273