Summary
The in vivo transcripts of the tobacco chloroplast gene for the beta subunit of the ATPase (atpB) were examined. In tobacco, like spinach, the atpB gene encodes multiple transcripts. Six tobacco atpB transcripts are present in vivo, with 5′ ends at positions “-90”, “-255”, “-290”, “-490”, “-500” and “-610” relative to the translation initiation site. The 5′ end of the atpB gene (“-610” position) is 20 base pairs from the 5′ end of the rbcL gene, coded for on the complementary strand. The “-255”, “-490” and “-610” regions are recognized as promoters in vitro by spinach chloroplast and E. coli RNA polymerases.
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References
Barkin A (1988) EMBO J 7:2637–2644
Bradley D, Gatenby AA (1985) EMBO J 4:3641–3648
Chen L-J, Orozco EM Jr (1988) Nucleic Acids Res 16:8411–8431
Chen L-J, Rogers SA, Bennett DC, Hu M-C, Orozco EM Jr (1990) Curr Genet 17:55–64
Crossland LD, Rodermel SR, Bogorad L (1984) Proc Natl Acad Sci USA 81:4060–4064
Fluhr R, Fromm H, Edelman E (1983) Gene 25:271–280
Gruissem W, Zurawski G (1985) EMBO J 4:3375–3385
Gruissem W, Barkan A, Deng X-W, Stern D (1988) Trends Genet 4:258–263
Hanley-Bowdoin L, Orozco EM Jr, Chua N-H (1985a) Mol Cell Biol 5:2733–2745
Hanley-Bowdoin L, Orozco EM Jr, Chua N-H (1985b) In: Steinback K, Bonitz S, Arntzen C, Bogorad L (eds) Molecular biology of the photosynthetic apparatus. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 311–318
Hanley-Bowdoin L, Chua N-H (1987) Trends Biochem Sci 12:67–70
Hollingsworth MJ, Johanningmeier U, Karabin GD, Stiegler GL, Hallick RB (1984) Nucleic Acids Res 12:2001–2017
Krebbers ET, Larrinua IM, McIntosh L, Bogorad L (1982) Nucleic Acids Res 10:4985–5002
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Maxam AM, Gilbert W (1980) Methods Enzymol 65:499–560
Mead DA, Szczesna-Skorupa E, Kemper B (1986) Protein Engineering 1:67–74
Mullet JE, Orozco EM Jr, Chua N-H (1985) Plant Mol Biol 4:39–54
Orozco EM Jr, Mullet JE, Chua N-H (1985) Nucleic Acids Res 13:1283–1302
Orozco EM Jr, Mullet JE, Hanley-Bowdoin L, Chua N-H (1986) Methods Enzymol 118:232–253
Shinozaki K, Sugiura M (1982) Nucleic Acids Res 10:4923–4934
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049
Stern DB, Gruissem W (1987) Cell 51:1145–1157
Sugiura M, Kusuda J (1979) Mol Gen Genet 172:137–141
Tanaka M, Obokata J, Chunwongse J, Shinozaki K, Sugiura M (1987) Mol Gen Genet 209:427–431
Westhoff P, Farchaus JW, Herrmann RG (1986) Curr Genet 11:165–169
Zurawski G, Bottomley W, Whitfeld PR (1982) Proc Natl Acad Sci USA 79:6260–6264
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Communicated by Barbara B. Sears
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Orozco, E.M., Chen, LJ. & Eilers, R.J. The divergently transcribed rbcL and atpB genes of tobacco plastid DNA are separated by nineteen base pairs. Curr Genet 17, 65–71 (1990). https://doi.org/10.1007/BF00313250
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DOI: https://doi.org/10.1007/BF00313250