Summary
A balanced polymorphism within the single chloroplast of the green alga Chlamydomonas reinhardtii has been postulated to account for stable heteroplasmicity. Heteroplasmicity of mutant and wild-type alleles of a suppressor gene appears to be required for the suppression of a nonsense mutation in the chloroplast large-subunit gene (rbcL) of ribulosebisphosphate carboxylase/oxygenase. Whereas nonsense suppression has been demonstrated, its molecular basis remains to be determined. In the present study, the suppressed strain was found to synthesize both truncated and full-length large-subunit proteins. Since the rbcL nonsense mutation created a new restriction enzyme site, restriction-enzyme analysis and DNA hybridization were employed to show that only the mutant gene was present in the suppressed strain. It is thus likely that informational suppression is involved in the heteroplasmic suppression mechanism: the original nonsense mutation is present, but normal enzyme is produced comprised of full-length large subunits.
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References
Birky CW (1983) Science 222:468–475
Bradford MM (1976) Anal Biochem 72:248–254
Chen Z, Chastain CJ, Al-Abed SR, Chollet R, Spreitzer RJ (1988) Proc Natl Acad Sci USA 85:4696–4699
Chua NH (1980) Methods Enzymol 69:434–446
Denhardt DT (1966) Biochem Biophys Res Comm 23:641–646
Dron M, Rahiré M, Rochaix JD (1982) J Mol Biol 162:775–793
Gillham NW (1978) Organelle heredity. Raven Press, New York
Hartman PE, Roth JR (1973) Adv Genet 17:1–105
Laipis PJ, Van de Walle M, Hauswirth WW (1988) Proc Natl Acad Sci USA 85:8107–8110
Laskey RA, Mills AD (1975) Eur J Biochem 56:335–341
Moon E, Kao TH, Wu R (1987) Nucleic Acid Res 15:611–630
Müller PP, Reif MK, Zonghou S, Sengstag C, Mason TL, Fox TD (1984) J Mol Biol 175:431–452
Rigby PW, Diekman JM, Rhodes C, Berg P (1977) J Mol Biol 113:237–251
Rochaix JD (1982) In: Edelman M, Hallick RB, Chua NH (eds) Methods in chloroplast molecular biology. Elsevier Science Publishers, Amsterdam, p 295
Rödel G, Fox TD (1987) Mol Gen Genet 206:45–50
Solignac M, Génermont J, Monnerot M, Mounolou JC (1987) Genetics 117:687–696
Southern EM (1975) J Mol Biol 98:503–517
Spreitzer RJ, Chastain CJ (1987) Curr Genet 11:611–616
Spreitzer RJ, Mets L (1981) Plant Physiol 67:565–569
Spreitzer RJ, Ogren WL (1983) Proc Natl Acad Sci USA 80:6293–6297
Spreitzer RJ, Chastain CJ, Ogren WL (1984) Curr Genet 9:83–89
Spreitzer RJ, Goldschmidt-Clermont M, Rahiré M, Rochaix JD (1985) Proc Natl Acad Sci USA 82:5460–5464
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Communicated by K. P. Van Winkle-Swift
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Zhang, D., Spreitzer, R.J. Evidence for informational suppression within the chloroplast of Chlamydomonas reinhardtii . Curr Genet 17, 49–53 (1990). https://doi.org/10.1007/BF00313248
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DOI: https://doi.org/10.1007/BF00313248