Skip to main content
SpringerLink
Log in

Conditional hyporecombination mutants of three REC genes of Saccharomyces cerevisiae

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

We have isolated and characterized three conditional hyporecombination mutants, rec1-1, rec3-1 and rec4-1, that define three REC genes of Saccharomyces cerevisiae required for spontaneous general mitotic interchromosomal recombination. Each MATa/MATα rec/rec diploid is deficient in mitotic single site gene conversion, intragenic recombination, intergenic recombination and sporulation at the restrictive temperature (36°C). The rec1-1 mutation also confers conditional enhanced sensitivity to the killing effects of X-rays. The rec1-1 and rec3-1 mutations have been mapped to chromosome VII. The rec1-1, rec3-1 and rec4-1 mutations exhibit complementation at 36°C for both mitotic recombination and sporulation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aguilera A, Klein HL (1988) Genetics 119:779–790

    Google Scholar 

  • Alberts BM (1984) Cold Spring Harbor Symp Quant Biol 49:1–12

    Google Scholar 

  • Boram WR, Roman H (1976) Proc Natl Acad Sci USA 73:2828–2832

    Google Scholar 

  • Bruschi CV, Howe GA (1988) Curr Genet 14:191–200

    Google Scholar 

  • Esposito MS (1984) Symp Soc Exper Biol 38:123–160

    Google Scholar 

  • Esposito MS, Hosoda J, Golin J, Moise H, Bjornstad K, Maleas D (1984) Cold Spring Harbor Symp Quant Biol 49:41–48

    Google Scholar 

  • Esposito MS, Maleas D, Bjornstad K, Holbrook LL (1986) Curr Genet 425–433

  • Fogel S, Roth R (1974) Mol Gen Genet 130:189–201

    Google Scholar 

  • Dresser ME, Giroux CN (1988) J Cell Biol 106:567–573

    Google Scholar 

  • Game JC, Johnston LH, von Borstel RC (1979) Proc Natl Acad Sci USA 76:4589–4592

    Google Scholar 

  • Game JC, Lamb TJ, Braun RJ, Resnick M, Roth M (1980) Genetics 94:51–68

    Google Scholar 

  • Golin JE, Esposito MS (1977) Mol Gen Genet 150:127–135

    Google Scholar 

  • Golin JE, Esposito MS (1981) Mol Gen Genet 183:252–263

    Google Scholar 

  • Hosoda J, Holbrook LL, Moise H, Bjornstad K, Maleas D, Esposito MS (1987) In: Stewart et al. (eds) Biological Research on Industrial Yeasts Vol. 2 CRC Press Inc, Fl pp 171–192

    Google Scholar 

  • Klapholz S, Waddell CS, Esposito RE (1985) Genetics 110:187–216

    Google Scholar 

  • Klein HL, Petes TD (1980) Nature 289:144–148

    Google Scholar 

  • Lea DE, Coulson CA (1948) J Genet 49:264–284

    Google Scholar 

  • Montelone BA, Hoekstra MF, Malone RE (1988) Genetics 119:289–301

    Google Scholar 

  • Mortimer RK, Schild D (1985) Microbiol Rev 49:181–212

    Google Scholar 

  • Orr-Weaver TL, Szostak JW, Rothstein RJ (1981) Proc Natl Acad Sci USA 78:6354–6358

    Google Scholar 

  • Rodarte-Ramon US, Mortimer RK (1972) Radiat Res 49:133–138

    Google Scholar 

  • Roeder GS, Stewart SE (1988) Trends Genetics 4:263–267

    Google Scholar 

  • Roman H (1956) Cold Spring Harbor Symp Quant Biol 12:175–185

    Google Scholar 

  • Rothstein RJ (1984) Cold Spring Harbor Symp Quant Biol 49:629–637

    Google Scholar 

  • Rudin N, Haber JE (1988) Mol Cell Biol 8:3918–3928

    Google Scholar 

  • Schiestl RH, Prakash S (1988) Mol Cell Biol 8:3619–3626

    Google Scholar 

  • West SC (1988) Trends Genetics 4:8–13

    Google Scholar 

  • Williamson MS, Game JC, Fogel S (1985) Genetics 110:609–646

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, 934-47A, University of California, 94720, Berkeley, CA, USA

    Michael S. Esposito & John T. Brown

Authors
  1. Michael S. Esposito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John T. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by Rodney J. Rothstein

Rights and permissions

Reprints and permissions

About this article

Cite this article

Esposito, M.S., Brown, J.T. Conditional hyporecombination mutants of three REC genes of Saccharomyces cerevisiae . Curr Genet 17, 7–12 (1990). https://doi.org/10.1007/BF00313242

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00313242

Key words

Search

Navigation