Summary
La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins with molecular weights of 38, 40 and 87 kDa were found for M2, M1 and L3 dsRNAs, respectively. The average G+C content of these dsRNAs was 43%, close to that of A. bisporus nuclear DNA. The nucleotide sequences, as well as the amino acid sequences, appear to be unique, as no matching sequences could be found among databases. S3 dsRNA (0.39 kb), which is occasionally found in large amounts in diseased mushrooms, is an internally deleted variant of M2 dsRNA and is largely composed of the non-coding ends of that dsRNA.
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Agellon LB, Chen TT (1986) Gene Anal Techn 3:86–89
Azad AA, Barrett SA, Fahey KJ (1985) Virology 143:35–44
Bailey JM, Davidson N (1976) Anal Biochem 70:75–85
Barton RJ, Hollings M (1979) J gen Virol 42:231–240
Birboim HC, Doly J (1979) Nucleic Acids Res 7:3–23
Bostian K, Bussey H, Elliot Q, Burn Q, Smith A, Tipper DJ (1984) Cell 36:741–751
Buck KW (1986) In: Buck KW (ed) Fungal virology. CRC Press, Boca Raton, Florida, pp 1–84
Chang T-H, Banerjee N, Bruenn J, Held W, Peery T (1988) Genes 2:195–206
Chou PY, Fasman GD (1978) Annu Rev Biochem 47:2:195–206
Chou PY, Fasman GD (1978) Annu Rev Biochem 47:251–276
Church GM, Gilbert W (1984) Proc Natl Acad Sci USA 81:1991–1995
Dalton RE, Podila GK, Flurkey WH Bozarth RF (1985) Virus Res 3:153–163
Doolitle RF (1986) Of URFs and ORFs; a primer on how to analyze derived amino acid sequences. University Science Books, Mill Valey, California
El-Sherheini M, Bostian KA, Mitchell DJ (1987) Curr Genet 11:483–490
Esteban R, Fujimura T, Wickner RB (1988) Proc Natl Acad Sci USA 85:4411–4415
Ghabrial SA, Bibb JA, Price WM, Lesnaw JA (1987) J gen Virol 68:1791–1800
Gubler U, Hoffmann BJ (1983) Gene 25:263–369
Gurr SJ, Unkles SE, Kinghorn JR (1987) In: Kinghorn JR (ed) Gene structure in eukaryotic microbes. IRL Press, Oxford, pp 93–139
Harmsen MC, van Griensven LJLD, Wessels JGH (1989) J gen Virol 70:1613–1616
Hiremath S, L'Hostis B, Ghabrial SA, Rhoads RE (1986) Nucleic Acids Res 14:9877–9896
Hopper JE, Bostian KA, Rowe LB, Tipper DJ (1977) J Biol Chem 252:9010–9017
Icho T, Wickner RB, (1989) J Biol Chem 264:6716–6723
Jang SK, Davies MV, Kaufman RJ, Wimmer E (1989) J Virol 83:1651–1660
Kozak M (1984) Nucleic Acids Res 12:857–872
Kozak M (1987) Nucleic Acids Res 15:8125–8133
Kyte J, Doolittle RF (1982) J Mol Biol 157:105–132
Lee M, Pietras DF, Nemeroff ME, Corstanje BJ, Field LJ, Bruenn JA (1986) J Virol 58:402–407
Leibowitz MJ, Hussain I, Williams TL (1988) In: Koltin Y, Leibowitz MJ (eds) Viruses of fungi and simple eukaryotes. Marcel Dekker, New York, pp 133–161
Lipman DJ, Pearson WR (1985) Science 227:1435–1441
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Marino R, Saksena KN, Schuler M, Mayfield JE, Lemke PA (1976) Appl Environ Microbiol 31:433–438
Pelletier J, Sonenberg N (1988) Nature 334:320–325
Rae BP, Hillman BI, Tartaglia J, Nuss DI (1989) EMBO J 8:657–663
Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467
Sharp PM, Cowe E, Higgins DG, Shields DC, Wolfe KH, Wright F (1988) Nucleic Acids Res 16:8207–8211
Sippel AE (1973) Eur J Biochem 37:31–40
Smith M, Leung DW, Gilliam S, Astell CR (1979) Cell 16:753–761
Stephens RM (1985) Gene Anal Techn 2:67–75
Tabor S, Richardson CC (1987) Proc Natl Acad Sci USA 84:4767–4771
Tao J, Ginsberg I, Banerjee N, Held W, Koltin Y, Bruenn JA (1990) Mol Cell Biol 10:1373–1381
Tartaglia J, Paul CP, Fulbright DW, Nuss DL (1986) Proc Natl Acad Sci USA 83:9109–9113
Tautz D, Renz M (1983) Anal Biochem 132:20–31
Thiele DJ, Hanning EM, Leibowitz MJ (1984) Virology 137:20–31
Tinoco I, Borer PN, Dengler B, Levine MD, Uhlenbeck OC, Crothers DM, Gralla J (1973) Nature (New Biology) 246:40–41
Wach MP, Romaine CP (1981) Phytopathology 71:911
Wach MP, Sriskantha A, Romaine CP (1987) Phytopathology 77:1321–1325
Zaayen A van (1979) In: Lemke PA (ed) Viruses and plasmids in fungi, Marcel Dekker, New York, pp 239–324
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Harmsen, M.C., Tolner, B., Kram, A. et al. Sequences of three dsRNAs associated with La France disease of the cultivated mushroom (Agaricus bisporus). Curr Genet 20, 137–144 (1991). https://doi.org/10.1007/BF00312776
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DOI: https://doi.org/10.1007/BF00312776