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Construction of a Saccharomyces cerevisiae strain able to ferment cellobiose

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Summary

The bglA gene, encoding a β-glucosidase from Bacillus polymyxa, has been expressed in Saccharomyces cerevisiae under control of the CYC-GAL promoter inducible by galactose. The expression of bglA-encoded activity in the strain used as a host was not sufficient to allow its growth with cellobiose as a carbon source. However, a recessive mutation in a gene designated cem1 has been obtained which, combined with the expression of β-glucosidase activity, allows the growth of S. cerevisiae on cellobiose. The expression of the bglA gene in a cemt strain confers on S. cerevisiae the capability for an efficient fermentation of cellobiose, as detected by the formation of CO2.

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Authors and Affiliations

  1. Instituto de Agroquímica y Tecnología de Alimentos, CSIC, Jaime Roig 11, E-46010, Valencia, Spain

    Ana C. Adam & Julio Polaina

Authors
  1. Ana C. Adam
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  2. Julio Polaina
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Communicated by F. K. Zimmermann

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Adam, A.C., Polaina, J. Construction of a Saccharomyces cerevisiae strain able to ferment cellobiose. Curr Genet 20, 5–8 (1991). https://doi.org/10.1007/BF00312758

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  • DOI: https://doi.org/10.1007/BF00312758

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