Summary
In Schizosaccharomyces pombe spontaneous deletions occur in the mating-type (MT) region; the latter comprises closely linked mat loci and intervening L and K regions. The loss of L is lethal; such deletions (e.g., h +L) can only be maintained in diploid strains. In tetrads of h +L/h -S strains we found some viable h +L spores yielding colonies with a weak iodine reaction. These wipI clones contain mat2:1° (M) plasmids derived from h -S. The L region of the plasmid can compensate the chromosomal h +L deletion. The episomal M cassette is also expressed. Furthermore, MT switching takes place in this cassette. The plasmids can integrate into the chromosomal MT region giving rise to h 90, h +R and some other configurations.
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References
Beach DH (1983) Nature 305: 683–688
Beach DH, Klar AJS (1984) EMBO J 3: 603–610
Beach DH, Nurse P, Egel R (1982) Nature 296: 682–683
Egel R (1984) Curr Genet 8: 199–203
Egel R (1989) In: Nasim A, Young P, Johnson BF (eds) Molecular biology of the fission yeast. Academic Press, San Diego, New York, pp 31–73
Egel R, Gutz H (1981) Curr Genet 3: 5–12
Engelke U, Grabowski L, Gutz H, Heim L, Schmidt H (1987) Curr Genet 12: 535–542
Fleck O, Heim L, Gutz H (1990) Curr Genet 18: 501–509
Gunge N (1983) Annu Rev Microbiol 37: 253–276
Gutz H, Angehrn P (1968) Genetics 60: 186 (abstract)
Gutz H, Doe FJ (1973) Genetics 74: 563–569
Gutz H, Schmidt H (1985) Curr Genet 9: 325–331
Gutz H, Schmidt H (1990) Sem Develop Biol 1: 169–176
Gutz H, Heslot H, Leupold U, Loprieno N (1974) In: King, RC (ed) Handbook of Genetics, vol. 1. Plenum Press, New York, pp 395–446
Haefner K (1967) Genetics 57: 169–178
Heim L (1986) Dissertation, Naturwissenschaftliche Fakultät, Technische Universität, Braunschweig
Heim L (1990) Curr Genet 17: 13–19
Heyer W-D, Sipiczki M, Kohli J (1986) Mol. Cell. Biol. 6: 80–89
Leupold U (1950) CR Trav Lab Carlsberg Sér Physiol 24: 381–480
Leupold U (1958) Cold Spring Harbor Symp Quant Biol 23: 161–170
Maniatis T, Jeffrey A, Kleid DG (1975) Proc Natl Acad Sci USA 72: 1184–1188
Nielsen O, Egel R (1989) EMBO J 8: 269–276
Stephen ER, Nasim A (1981) Can J Microbiol 27: 550–553
Strathern JN, Klar AJS, Hicks JB, Abraham JA, Ivy JM, Nasmith KA, McGill C (1982) Cell 31: 183–192
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Communicated by K. Wolf
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Heim, L., Gutz, H. Rescue of lethal mutations in the mating-type region of Schizosaccharomyces pombe by an extrachromosomal element. Curr Genet 19, 435–444 (1991). https://doi.org/10.1007/BF00312734
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DOI: https://doi.org/10.1007/BF00312734