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Current Genetics

, Volume 23, Issue 5–6, pp 547–548 | Cite as

Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2

  • Guillaume Cottarel
  • David Beach
  • Ulrich Deuschle
Short Communications

Abstract

Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.

Key words

Autonomously replicating sequence Recombinant DNA Nested deletions Mutagenesis Schizosaccharomyces pombe 

References

  1. Beach D, Nurse P (1981) Nature 290:140–142Google Scholar
  2. Beach D, Durkacz B, Nurse P (1982a) Nature 300:706–709Google Scholar
  3. Beach D, Piper M, Nurse P (1982b) Mol Gen Genet 187:326–329Google Scholar
  4. Broach JR, Strathern JN, Hicks JB (1979) Gene 8:121–133Google Scholar
  5. Gaillardin C, Fournier P, Budar F, Kudla B, Gerbaud C, Heslot H (1983) Curr Genet 7:245–253Google Scholar
  6. Henikoff S (1984) Gene 28:351–359Google Scholar
  7. Henikoff S (1990) Nucleic Acids Res 18:2961–2966Google Scholar
  8. Heyer W-D, Sipiczki M, Kohli J (1986) Mol Cell Biol 6:80–89Google Scholar
  9. Hindley J, Phear G, Stein M, Beach D (1987) Mol Cell Biol 7:504–511Google Scholar
  10. Losson R, Lacroute F (1983) Cell 32:371–377Google Scholar
  11. Matsumoto T, Beach D (1991) Cell 66:347–360Google Scholar
  12. Maundrell K, Hutchinson A, Shall S (1988) EMBO J 7:2203–2209Google Scholar
  13. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  14. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  15. Sikorski RS, Hieter P (1989) Genetics 122:19–27Google Scholar
  16. Weilguny D, Prætorius M, Carr A, Egel R, Nielsen O (1991) Gene 99:47–54Google Scholar
  17. Wright A, Maundrell K, Heyer W-D, Beach D, Nurse P (1986) Plasmid 15:156–158Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Guillaume Cottarel
    • 1
  • David Beach
    • 1
  • Ulrich Deuschle
    • 1
  1. 1.Cold Spring Harbor LaboratoryHoward Hughes Medical InstituteCold Spring HarborUSA

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