Current Genetics

, Volume 23, Issue 5–6, pp 547–548 | Cite as

Two new multi-purpose multicopy Schizosaccharomyces pombe shuttle vectors, pSP1 and pSP2

  • Guillaume Cottarel
  • David Beach
  • Ulrich Deuschle
Short Communications


Plasmids pSP1 and pSP2 are two new Schizosaccharomyces pombe ars1 multicopy vectors with the Saccharomyces cerevisiae LEU2 and URA3 genes as selectable markers. They are derivatives of S. cerevisiae integrative plasmids. These plasmids allow classical molecular genetic techniques, such as mutagenesis, nested deletions and sequencing, to be performed directly.

Key words

Autonomously replicating sequence Recombinant DNA Nested deletions Mutagenesis Schizosaccharomyces pombe 


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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Guillaume Cottarel
    • 1
  • David Beach
    • 1
  • Ulrich Deuschle
    • 1
  1. 1.Cold Spring Harbor LaboratoryHoward Hughes Medical InstituteCold Spring HarborUSA

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