Abstract
The genes encoding subunits 1, 3, 4L, and 6 of NADH dehydrogenase (nad1, nad3, nad4L, nad6) in the mitochondrial genome of a liverwort, Marchantia polymorpha, were characterized by comparing homologies of the amino-acid sequences of the subunits with those of other organisms. The nad3 and nad4L genes are split by single and double group II introns, respectively. The 5′-half portion of the nad6 gene was repeated at an identity of 89% to form a reading frame consisting of 100 amino-acid residues. The Northern hybridization analysis showed that all four genes are transcribed in the liverwort mitochondria.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current protocols in molecular biology. Greene Publishing Associates and Wiley-Interscience, Secaucus, New Jersey, p 4.3.2
Biswas TK, Ticho B, Getz GS (1987) J Biol Chem 262:13690–13696
Binder S, Marchfelder A, Brennicke A, Wissinger B (1992) J Biol Chem 267:7615–7623
Bland MM, Levings CS III, Matzinger DF (1986) Mol Gen Genet 204:8–16
Brears T, Lonsdale DM (1988) Mol Gen Genet 214:514–522
Brown GG, Auchincloss AH, Covello PS, Gray MW, Menassa R, Singh M (1991) Mol Gen Genet 228:345–355
Chapdelaine Y, Bonen L (1991) Cell 65:465–472
Chomyn A, Mariottini P, Cleeter MWJ, Ragan CI, Matsuno-Yagi A, Hatefi Y, Doolittle RF, Attardi G (1985) Nature 314:592–597
Chomyn A, Cleeter MWJ, Ragan CI, Riley M, Doolittle RF, Attardi G (1986) Science 234:614–618
Conklin PL, Wilson RK, Hanson MR (1991) Genes Dev 5:1407–1415
Covello PS, Gray MW (1991) Curr Genet 20:245–251
Cummings DJ, McNally KL, Domenico JM, Matsuura ET (1990) Curr Genet 17:375–402
Dupuis A, Skehel JM, Walker JE (1991) Biochemistry 30:2954–2960
Ecke W, Schmitz U, Michaelis G (1990) Curr Genet 18:133–139
Gass DA, Makaroff CA, Palmer JD (1992) Curr Genet 21:423–430
Gualberto JM, Wintz H, Weil J-H, Grienenberger JM (1988) Mol Gen Genet 215:118–127
Gualberto JM, Lamattina L, Bonnard G, Weil JH, Grienenberger JM (1989) Nature 341:660–662
Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194
Knoop V, Schuster W, Wissinger B, Brennicke A (1991) EMBO J 10:3483–3493
Kohchi T, Shirai H, Fukuzawa H, Sano T, Komano T, Umesono K, Inokuchi H, Ozeki H, Ohyama K (1988) J Mol Biol 203:353–372
Lamattina L, Grienenberger JM (1991) Nucleic Acids Res 19:3275–3282
Lamattina L, Weil JH, Grienenberger JM (1989) FEBS Lett 258:79–89
Lonsdale DM (1989) In: Stumpf PK, Conn EE (eds) The biochemistry of plants, vol 15. Academic Press, New York, pp 229–295
Makaroff CA, Palmer JD (1987) Nucleic Acids Res 15:5141–5156
Mulligan RM, Lau GT, Walbot V (1988a) Proc Natl Acad Sci USA 85:7998–8002
Mulligan RM, Maloney AP, Walbot V (1988b) Mol Gen Genet 211:373–380
Nixon PJ, Gounaris K, Coomber SA, Hunter CN, Dyer TA, Barber J (1989) J Biol Chem 264:14129–14135
Nozato N, Oda K, Yamato Y, Ohta E, Takemura M, Akashi K, Fukuzawa H, Ohyama K (1992) Mol Gen Genet (in press)
Oda K, Kohchi T, Ohyama K (1992a) Biosci Biotech Biochem 56:132–135
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K (1992b) J Mol Biol 223:1–7
Oda K, Yamato K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K (1992c) Plant Mol Biol Rep 10:105–163
Ohyama K, Fukuzawa H, Kohchi T, Sano T, Sano S, Shirai H, Umesono K, Shiki T, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1988) J Mol Biol 203:281–298
Palmer JD, Herbon LA (1988) J Mol Evol 28: 87–97
Pereira de Souza A, Jubier M-F, Delcher E, Lancelin D, Lejeune B (1991) Plant Cell 3: 1363–1378
Rasmussen J, Hanson MR (1989) Mol Gen Genet 215: 332–336
Rothenberg M, Hanson MR (1987) Mol Gen Genet 209: 21–27
Schuster W, Hiesel R, Isaac PG, Leaver CJ, Brennicke A (1986) Nucleic Acids Res 14: 5943–5954
Schuster W, Wissinger B, Unseld M, Brennicke A (1990) EMBO J 9: 263–269
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5: 2043–2049
Siculella L, Palmer JD (1988) Nucleic Acids Res 16: 3787–3799
Stern DB, Newton KJ (1984) Plant Mol Biol Rep 2: 8–15
Stern DB, Palmer JD (1986) Nucleic Acids Res 14: 5651–5666
Stern DB, Bang AG, Thompson WF (1986) Curr Genet 10: 857–869
Umesono K, Inokuchi H, Shiki Y, Takeuchi M, Chang Z, Fukuzawa H, Kohchi T, Shirai H, Ohyama K, Ozeki H (1988) J Mol Biol 203: 299–331
Wahleithner JA, MacFarlane JL, Wolstenholme DR (1990) Proc Natl Acad Sci USA 87: 548–552
Wintz H, Chen H-C, Pillay DTN (1989) Curr Genet 15: 155–160
Wissinger B, Hiesel R, Schuster W, Brennicke A (1988) Mol Gen Genet 212: 56–65
Wissinger B, Schuster W, Brennicke A (1991) Cell 65: 473–482
Xue Y, Davies DR, Thomas CM (1990) Mol Gen Genet 221: 195–198
Yamato K, Ogura Y, Kanegae T, Yamada Y, Ohyama K (1992) Theor Appl Genet 83: 279–288
Young EG, Hanson MR, Dierks PM (1986) Nucleic Acids Res 14: 7995–8006
Author information
Authors and Affiliations
Additional information
Communicated by H. Kössel
Rights and permissions
About this article
Cite this article
Yamato, K., Nozato, N., Oda, K. et al. Occurrence and transcription of genes for nad1, nad3, nad4L, and nad6, coding for NADH dehydrogenase subunits 1, 3, 4L, and 6, in liverwort mitochondria. Curr Genet 23, 526–531 (1993). https://doi.org/10.1007/BF00312646
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00312646