Current Genetics

, Volume 23, Issue 5–6, pp 382–387 | Cite as

Lack of correlation between trehalase activation and trehalose-6 phosphate synthase deactivation in cAMP-altered mutants of Saccharomyces cerevisiae

  • Juan-Carlos Argüelles
  • Dolores Carrillo
  • Jerónima Vicente-Soler
  • Francisco Garcia-Carmona
  • Mariano Gacto
Original Articles


The rise in cAMP level that follows the addition of glucose or 2,4-dinitrophenol (DNP) to stationaryphase cells of Saccharomyces cerevisiae was accompanied by a marked activation of trehalase (3-fold increase) and a concomitant deactivation of trehalose-6 phosphate synthase (50% of the basal levels). In glucose-grown exponential cells, which are deficient in glucose-induced cAMP signalling, the addition of glucose also prompted a decrease in trehalose-6 phosphate synthase, but had no effect on trehalase activity. Mutants defective in the RAS-adenylate cyclase pathway (ras1 ras2 bcy1 strain), as well as mutants containing greatly reduced protein kinase activity either cAMP-dependent (tpkw1BCY1 strains) or cAMP-independent (tpk1w1bcy1 strains), were unable to show glucose- or DNP-induced trehalase activation but still displayed a clear decrease in trehalose-6 phosphate synthase activity upon addition of these compounds. These data suggest that the activity of trehalose-6 phosphate synthase, as opposed to that of trehalase, is not controlled by the cAMP signalling pathway “in vivo”. Trehalose-6 phosphate synthase was competitively inhibited by glucose (Ki=15 mM) and resulted unaffected by ATP in assays performed “in vitro”.

Key words

Trehalase Trehalose-6-P synthase cAMP mutants Saccharomyces cerevisiae 


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  1. Argüelles JC, Gacto M (1985) Can J Microbiol 31:529–537Google Scholar
  2. Argüelles JC, Mbonyi K, Van Aelst L, Vanhalewyn M, Jans AWH, Thevelein JM (1990) Arch Microbiol 154:199–205Google Scholar
  3. Becker JV, Betz A (1972) Biochim Biophys Acta 274:584–597Google Scholar
  4. Cabib E, Leloir L (1958) J Biol Chem 231:259–275Google Scholar
  5. Cameron S, Levin L, Zoller M, Wigler M (1988) Cell 53:555–566Google Scholar
  6. Elbein AD (1974) Adv Carbohydr Chem 30:227–256Google Scholar
  7. François J, Neves MJ, Hers HG (1991) Yeast 7:575–587Google Scholar
  8. Lillie SH, Pringle JR (1980) J Bacteriol 143:1384–1394Google Scholar
  9. Londesborough J, Varimo K (1984) Biochem J 219:511–518Google Scholar
  10. Londesborough J, Vuorio O (1991) J Gen Microbiol 137:323–330Google Scholar
  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–275Google Scholar
  12. Mbonyi K, Van Aelst L, Argüelles JC, Jans AWH, Thevelein JM (1990) Mol Cell Biol 10:4518–4523Google Scholar
  13. Nikawa J, Cameron S, Toda T, Ferguson KM, Wigler M (1987) Genes Dev 1:931–937Google Scholar
  14. Ortiz CH, Maia JCC, Tenan MN, Braz-Padrao GR, Mattoon JR, Panek AD (1983) J Bacteriol 153:644–651Google Scholar
  15. Panek AC, Araujo PS, Moura Neto V, Panek AD (1987) Curr Genet 11:459–465Google Scholar
  16. Panek AC, Araujo PS, Poppe SC, Panek AD (1990) Biochem Int 21:695–704Google Scholar
  17. Thevelein JM (1988) Exp Mycol 12:1–12Google Scholar
  18. Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, Cameron S, Broach J, Matsumoto K, Wigler M (1985) Cell 40:27–36Google Scholar
  19. Toda T, Cameron S, Sass P, Zoller M, Scott JD, McMullen B, Hurwitz M, Krebs EG, Wigler M (1987a) Mol Cell Biol 7:1371–1377Google Scholar
  20. Toda T, Cameron S, Sass P, Zoller M, Wigler M (1987b) Cell 50:277–287Google Scholar
  21. Uno I, Matsumoto K, Adachi K, Ishikawa T (1983) J Biol Chem 258:10867–10872Google Scholar
  22. Van Laere A (1989) FEMS Microbiol Rev 63:201–210Google Scholar
  23. Vandercammen A, François J, Hers HG (1989) Eur J Biochem 182:613–620Google Scholar
  24. Vicente-Soler J, Argüelles JC, Gacto M (1989) FEMS Microbiol Lett 61:273–278Google Scholar
  25. Vicente-Soler J, Argüelles JC, Gacto M (1991) FEMS Microbiol Lett 82:157–162Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Juan-Carlos Argüelles
    • 1
  • Dolores Carrillo
    • 1
  • Jerónima Vicente-Soler
    • 1
  • Francisco Garcia-Carmona
    • 2
  • Mariano Gacto
    • 1
  1. 1.Departamento de Genética y Microbiologia, Facultad de BiologíaUniversidad de MurciaMurciaSpain
  2. 2.Departamento de Bioquimica, Facultad de BiologíaUniversidad de MurciaMurciaSpain

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