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Structure and regulation of the isocitrate lyase gene ICL1 from the yeast Saccharomyces cerevisiae

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Abstract

The ICL1 gene encoding the isocitrate lyase from Saccharomyces cerevisiae was cloned and sequenced. A reading frame of 557 amino acids showing significant similarity to isocitrate lyases from seven other species could be identified. Construction of icl1 null mutants led to growth defects on C2 carbon sources while utilization of sugars or C3 substrates remained unaffected. Using an ICL1-lacZ fusion integrated at the ICL1 locus, a more than 200-fold induction of β-galactosidase activity was observed after growth on ethanol when compared with glucose-repressed conditions. A preliminary analysis of the ICL1 upstream region identified a 364-bp fragment necessary and sufficient for this regulatory phenotype. Sequence motifs also present in the upstream regions of co-regulated genes were found within this region.

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References

  • Aitchison JD, Murray WW, Rachubinski RA (1991) J Biol Chem 266:23197–23203

    Google Scholar 

  • Atomi H, Ueda M, Hikido H, Hishida T, Teranishi Y, Tanaka A (1990) J Biochem 107:262–266

    Google Scholar 

  • Barnett JA, Kornberg HL (1960) J Gen Microbiol 23:65–82

    Google Scholar 

  • Beeching JR, Northcote DH (1987) Plant Mol Biol 8:471–475

    Google Scholar 

  • Carlson M, Botstein D (1982) Cell 28:145–154

    Google Scholar 

  • Celenza JL, Carlson M (1986) Science 233:1175–1180

    Google Scholar 

  • Celenza JL, Eng FJ, Carlson M (1989) Mol Cell Biol 9:5045–5054

    Google Scholar 

  • Cigan AM, Donahue TF (1987) Gene 59:1–18

    Google Scholar 

  • Ciriacy M (1977) Mol Gen Genet 154:213–220

    Google Scholar 

  • Comai L, Dietrich RA, Maslyan DJ, Baden CS, Harada JJ (1989) Plant Cell 1:293–300

    Google Scholar 

  • Einerhand AWC, Voorn-Brouwer TM, Erdmann R, Kunau WH, Tabak HF (1991) Eur J Biochem 200:113–122

    Google Scholar 

  • Entian KD, Zimmermann FK (1982) J Bacteriol 151:1123–1128

    Google Scholar 

  • Fernandez E, Moreno F, Rodicio R (1992) Eur J Biochem 204:983–990

    Google Scholar 

  • Fitzgerald M, Shenk T (1981) Cell 24:251–260

    Google Scholar 

  • Forsburg SL, Guarente L (1988) Mol Cell Biol 8:647–654

    Google Scholar 

  • Gainey LDS, Connerton IF, Lewis EH, Turner G, Ballance DJ (1992) Curr Genet 21:43–47

    Google Scholar 

  • Gancedo JM (1992) Eur J Biochem 206:297–313

    Google Scholar 

  • Gietz RD, Sugino A (1988) Gene 74:527–534

    Google Scholar 

  • Gonzalez E (1977) J Bacteriol 129:1343–1348

    Google Scholar 

  • Gould SJ, Keller GA, Hosken N, Wilkinson J, Subramani S (1989) J Cell Biol 108:1657–1664

    Google Scholar 

  • Holzer H (1976) Trends Biochem Sci 1:178–181

    Google Scholar 

  • Ko YH, McFadden BA (1990) Arch Biochem Biophys 278:373–380

    Google Scholar 

  • Ko YH, Cremo CR, McFadden BA (1992) J Biol Chem 267:91–95

    Google Scholar 

  • Lopez-Boado YS, Herrero P, Gascon S, Moreno F (1987) Arch Microbiol 147:231–234

    Google Scholar 

  • Lopez-Boado YS, Herrero P, Fernandez T, Fernandez R, Moreno F (1988a) Yeast 4:41–46

    Google Scholar 

  • Lopez-Boado YS, Herrero P, Fernandez T, Fernandez R, Moreno F (1988b) J Gen Microbiol 134:2499–2505

    Google Scholar 

  • Matsuoka M, McFadden BA (1988) J Bacteriol 170:4528–4536

    Google Scholar 

  • McNeil JB, Smith M (1986) J Mol Biol 187:363–378

    Google Scholar 

  • Mercado JJ, Vincent O, Gancedo JM (1991) FEBS Lett 291:97–100

    Google Scholar 

  • Myers AM, Tzagoloff A, Kinney DM, Lusty CJ (1986) Gene 45:299–310

    Google Scholar 

  • Neigeborn L, Carlson M (1984) Genetics 108:845–858

    Google Scholar 

  • Nehlin JO, Ronne H (1990) EMBO J 9:2891–2898

    Google Scholar 

  • Nehlin JO, Carlberg M, Ronne H (1991) EMBO J 10:3373–3377

    Google Scholar 

  • Osborne BI, Guarente L (1989) Proc Natl Acad Sci USA 86:4097–4101

    Google Scholar 

  • Rieul C, Bleicher F, Duclos B, Cortay JC, Cozzone AJ (1988) Nucleic Acids Res 16:5689

    Google Scholar 

  • Rogers DT, Hiller E, Mitsock L, Orr E (1988) J Biol Chem 263:6051–6057

    Google Scholar 

  • Russell DW, Smith M, Williamson VM, Young ET (1983) J Biol Chem 258:2674–2682

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Sarokin L, Carlson M (1986) Mol Cell Biol 6:2324–2333

    Google Scholar 

  • Schüller HJ, Entian KD (1987) Mol Gen Genet 209:366–373

    Google Scholar 

  • Schüller HJ, Entian KD (1991) J Bacteriol 173:2045–2052

    Google Scholar 

  • Schüller HJ, Hahn A, Tröster F, Schütz A, Schweizer E (1992) EMBO J 11:107–114

    Google Scholar 

  • Simon M, Adam G, Rapatz W, Spevak W, Ruis H (1991) Mol Cell Biol 11:699–704

    Google Scholar 

  • Trumbly RJ (1992) Mol Microbiol 6:15–21

    Google Scholar 

  • Turley RB, Choe SM, Trelease RN (1990) Biochim Biophys Acta 1049:223–226

    Google Scholar 

  • Veenhuis M, Harder W (1991) In: Rose AH, Harrison JS (eds) The Yeasts, vol 4. Academic Press, London, pp 601–653

    Google Scholar 

  • Witt I, Kronau R, Holzer H (1968) Biochim Biophys Acta 118:522–537

    Google Scholar 

  • Zaret KS, Sherman F (1982) Cell 28:563–573

    Google Scholar 

  • Zimmermann FK, Kaufmann I, Rasenberger H, Haussmann P (1977) Mol Gen Genet 151:95–103

    Google Scholar 

Download references

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  1. Institut für Mikrobiologie und Biochemie, Staudtstrasse 5, W-8520, Erlangen, Germany

    A. Schöler & H. -J. Schüller

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  1. A. Schöler
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  2. H. -J. Schüller
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Communicated by F. K. Zimmermann

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Schöler, A., Schüller, H.J. Structure and regulation of the isocitrate lyase gene ICL1 from the yeast Saccharomyces cerevisiae . Curr Genet 23, 375–381 (1993). https://doi.org/10.1007/BF00312621

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  • DOI: https://doi.org/10.1007/BF00312621

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