Summary
We studied two polymorphic forms of mtDNA extracted from A. lixula eggs. In order to compare and to quantitate the variability, we sequenced specific regions of the two molecules. In this way, we obtained a precise measurement of the variability within two haplotypes. We also obtained a direct demonstration that some differences in nucleotide sequence can escape detection when restriction endonuclease analysis is used. Our results underline the unreliability of the use of restriction mapping to estimate divergence between relatively short and closely related DNA sequences.
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References
De Giorgi C (1988) Cell Biol Inter Reports 12:407–412
Nei M, Tajima F (1983) Genetics 105:407–412
Roberts R (1989) Nucleic Acids Res r17:347–387
Wilson AC, Cann RL, Carr SM, George M, Gyllensten UV, HelmBychowski KM, Higuchi RG, Palumbi SR, Prager EM, Sage RD, Stoneking M (1985) Biol J Linnean Soc 26:375–400
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De Giorgi, C., De Luca, F., Stefanile, C. et al. Direct evidence that restriction endonucleases may under estimate the degree of divergence between molecules. Curr Genet 18, 167–168 (1990). https://doi.org/10.1007/BF00312605
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DOI: https://doi.org/10.1007/BF00312605