Skip to main content
SpringerLink
Log in

Cell kinetics in the spleen of ehrlich ascites tumor-bearing mice

  • Original Papers
  • Published:
Zeitschrift für Krebsforschung und Klinische Onkologie Aims and scope Submit manuscript

Summary

During the period of 18 days after transplantation the uptake of 3H-thymidine into DNA was significantly increased in the spleen of Ehrlich ascites tumor-bearing Swiss and NMRI mice. Adenosine deaminase activity was within the control limits in tumor-bearing Swiss mice and significantly increased for the tumor-bearers of the NMRI strain. However, the increase in the DNA content, which is associated to splenic hyperplasia, was evident only for the Swiss strain. The time course of the retention of 3H-DNA revealed that the half life of spleen cells ranged from 41 to 46 h in normal and tumor-bearing Swiss mice and normal NMRI mice. For tumor-bearing NMRI mice the rate of cell depletion was significantly increased (t 1/2=32 h) compared with that found in normal NMRI mice (t 1/2=44h). The mean survival time was 23.0 days for the tumor-bearing Swiss mice and 24.6 days for the NMRI mice. These data suggest that the immune responses were similar in both strains of mice, although cell kinetics was different in the spleen of tumor-bearing Swiss and NMRI mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Carson,D.A., Goldblum,J.E., Seegmiller,J. E.: Quantitative immunoassay of adenosine deaminase in combined immunodeficiency disease. J. Immunol. 118, 270 (1977)

    Google Scholar 

  • Dissing,J., Knudsen,B: Adenosine deaminase deficiency and combined immunodeficiency syndrome. Lancet ii, 1316 (1972)

    Google Scholar 

  • Ertl,H.H., Feinedegen,L.E., Heiniger,H.J.: Iodine 125, a tracer in cell biology: Physical properties and biological aspects. Phys. Med. Biol. 15, 447 (1970)

    Google Scholar 

  • Giblett,E.R., Anderson,J.E., Cohen,F., Pollara,B., Meuwissen,H.J.: Adenosine deaminase deficiency in two patients with severe impaired cellular immunity. Lancet ii, 1067 (1972)

    Google Scholar 

  • Hovi,T., Smyth,J.F., Allison,A.C, Williams, S.C.: Role of adenosine deaminase in lymphocyte proliferation. Clin. Exp. Immunol. 23, 395 (1976)

    Google Scholar 

  • Hughes,W.L., Commerford,S.L., Gitlin,D., Kreuger,R.C, Schulze,B. Shah,V., Reilly,P.: Deoxyribonucleic acid metabolism in vivo. I. Cell proliferation and death as measured by incorporation and elimination of idodeoxyuridine. Fed. Proc. 23, 640 (1964)

    Google Scholar 

  • Joel,D.D., Chanana,A.D., Cottier,H., Cronkite,E.P., Laissue,J.A.: Fate of thymocytes: Studies with 125I-iododeoxyuridine and 3H-thymidine in mice. Cell Tissue Kinet. 10, 57 (1977)

    Google Scholar 

  • Konda,S., Nakao,Y., Smith,R. T.: The stimulating effect of tumor bearing upon T-and B-cell subpopulations of the mouse spleen. Cancer Res. 33, 2247 (1973)

    Google Scholar 

  • Konda,S., Smith,R.T.: The effects of tumor bearing upon changes in cell distribution and membrane antigen characteristics in murine spleen and thymus cell subpopulations. Cancer Res. 33, 1878 (1973)

    Google Scholar 

  • Morgan,W. W., Cameron,I.L.: Effect of fast-growing transplantable hepatoma on cell proliferation in host tissue of the mouse. Cancer Res. 33, 441 (1973)

    Google Scholar 

  • Nygaard,O.F., Potter,R.L.: Effect of X-radiation on DNA metabolism in various tissues of the rat. III. Retention of labelled DNA in normal and irradiated animals. Radiat. Res. 12, 131 (1960)

    Google Scholar 

  • Puck,T. T.: Cellular aspects of the mammalian radiation syndrome. II. Cell depletion in bone marrow, spleen and thymus of young mice.Radiat. Res. 27, 272 (1966)

    Google Scholar 

  • Sanders,J.L., Dalrymple,G.V., Robinette,C. D.: Retention of labelled deoxyribonucleic acid following irradiation: A statistical consideration. Nature (Lond.) 201, 206 (1963)

    Google Scholar 

  • Shirasaka,T., Fujii,S.: DNA synthesis in tumor-bearing rats. Cancer Res. 35, 517 (1975)

    Google Scholar 

  • Siegler,R., Koprowska,I.: Host responses to a transplantable “ascitic” tumor. Cancer Res. 22, 1278 (1962)

    Google Scholar 

  • Suciu,D., Uray,Z., Abraham,A.D.: Evidence for reproductive death of dividing cells in the thymus and spleen of whole-body gamma-irradiated mice. Int. J. Radiat. Biol. 28, 409 (1975)

    Google Scholar 

  • Suciu D., Uray,Z., Maniu,M.: Recovery response of dividing cells in the thymus of whole-body gamma-irradiated mice. Int. J. Radiat. Biol. 30, 409 (1976)

    Google Scholar 

  • Ullman, B., Cohen, A., Martin, D.W. jr.: Characterization of a cell culture model for the study of adenosine deaminase and purine nucleotide phosphorylase-deficient immunologic diseases. Cell 9, 205 (1976)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Oncological Institute, Republicii 34-36, 3400, Cluj-Napoca, Rumania

    D. Suciu & Z. Uray

Authors
  1. D. Suciu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. Uray
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Suciu, D., Uray, Z. Cell kinetics in the spleen of ehrlich ascites tumor-bearing mice. Z. Krebsforsch. 91, 261–266 (1978). https://doi.org/10.1007/BF00312288

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00312288

Key words

Search

Navigation