Abstract
The role of lymphocyte surface binding sites for wheat germ agglutinin (WGA) in the negative regulation of cancer patients was investigated. The number of WGA binding sites on the surface of each lymphocyte ranged from 107 to 108. Fluorescein isothiocyanate (FITC)-conjugated WGA, bound to the majority of peripheral blood lymphocytes (PBL) with two peaks of fluorescent intensity was expressed either dimly or brightly. The increase in lymphocytes brightly expressing WGA fluorescent intensity (WGA bright lymphocytes) significantly correlated with the number of WGA binding sites. The suppression of lymphocyte proliferation mediated by the purified soluble suppressor factor (SSF) significantly correlated with an increase in the WGA bright lymphocyte population (P<0.05). A significantly greater number of WGA bright lymphocytes in PBL was found in patients with esophageal, gastric, breast, or colon cancer, than in those with benign diseases or in healthy controls. Furthermore, an increase in WGA bright lymphocytes was found in subsets expressing the antigens CD8 dimly or CD16. Thus, it is suggested that the number of WGA binding sites may increase mainly on the surface of effector cells such as NK cells and CD8-positive killer T cells in cancer patients, triggering the negative regulation mediated by SSF.
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References
Boldt DH, Lyons RD (1979) Fractionation of human lymphocytes with plant lectins. II. Lens culinaris lectin and wheat germ agglutinin identify distinct lymphocyte subclasses. J Immunol 123:808–816
Boldt DH (1980) Interaction of wheat germ agglutinin with human peripheral blood mononuclear cells. Binding kinetic and flow microfluorometric analysis. Mol Immunol 17:47–55
Clement LT, Grossi CE, Gartland G (1984) Morphologic and phenotypic features of the subpopulation of Leu 2+ cells that suppresses B cell differentiation. J Immunol 133:2461–2468
Toge T, Yanagawa E, Nakanishi K, Yamada Y, Niimoto M, Hattori T (1980) Concanavalin-A activated suppressor cell activity in gastric cancer. Gann 71:784–789
Toge T, Hamamoto S, Itagaki E, Yajima K, Tanada M, Nakane H, Nakanishi K, Hattori T (1983) Concanavalin-A induced and spontaneous suppressor cell activities in peripheral blood lymphocytes and spleen cells from gastric cancer patients. Cancer 52:1624–1631
Toge T, Seto Y, Kuroi K, Yamada H, Aratani K, Fujita T, Yanagawa E, Hattori T (1987) Relationship of the distribution of Leu2+ cells with suppressor cell activities in the spleen and lymphocytes from gastric cancer. Jpn J Surg 17:72–77
Toge T, Kuroi K, Kuninobu H, Yamaguchi Y, Kegoya K, Baba N, Hattori T (1988) Role of the spleen on immunosuppression of gastric cancer: Predominance of suppressor precursors and suppressor inducer T cells in the recirculating spleen cells. Clin Exp Immunol 74:409–412
Williams RC, Korsmeyer SJ (1978) Studies on human lymphocyte interaction with emphasis on a soluble suppressor activity. Clin Immunol Immunopathol 9:335–349
Greene WC, Fleisher TA, Waldmann TA (1981) Soluble suppressor supernatants elaborated by concanavalin-A activated human mononuclear cells. 1. Characterization of a soluble suppressor of T cell proliferation. J Immunol 126:1185–1191
Boldt DH, MacDermott RP, Jorolan EP (1975) Interaction of plant lectins with purified human lymphocyte populations: Binding characteristics and kinetics of proliferation. J Immunol 114:1532–1536
Steck TL, Wallach DFH (1965) The binding of kidney-bean phytohemagglutinin by Ehrlich ascites carcinoma. Biochem Biophys Acta 97:510–522.
Nagata Y, Burger NM (1974) Wheat germ agglutinin. J Biol Chem 249:3116–3122
Oi VY, Glazer AN, Styryer L (1982) Fluorescent phycobiliprotein conjugate for analysis of cells and molecules. J Cell Biol 93:981–986
Toge T, Kameda A, Hattori T (1985) Effect of protein-bound polysaccharide on the soluble suppressor factors. In: Ishigami K (ed) Recent advances in chemotherapy, Anticancer Section. University of Tokyo Press, Tokyo, pp 755–756
Toge T, Nagusa Y, Nakano A, Ikeda H, Hattori T (1979) Lymphocyte responsiveness to phytohemagglutinin and its modification by serum inhibitor in breast cancer patients. Gann 70:245–248
Parker CW, Dankner RE, Folkenheim SF, Greene WC (1976) Suppressive evidence for both stimulatory and inhibitory domains on human lymphocyte membranes as indicated by phospholipid turnover with wheat germ agglutinin and other lectins. Immunol Commun 5:13–25
Greene WC, Walmann TA (1980) Inhibition of human lymphocyte proliferation by the nonmitogenic lectin wheat germ agglutinin. J Immunol 124:2979–2987
Greene WC, Parker CM, Parker CW (1976) Opposing effects of mitogenic and nonmitogenic lectins on lymphocyte activation. Evidence that wheat germ agglutinin produces negative signals. J Biol Chem 251:4017–4025
Takada S, Engleman EG (1987) Evidence for an association between CD8 molecules and the T cell receptor complex on cytotoxic T cell. J Immunol 139:3231–3235
Toge T, Yamaguchi Y, Kegoya Y, Baba N, Yanagawa E, Hattori T (1989) Blocking of lymphocyte surface binding sites for the soluble suppressor factor by protein bound polysaccharide, PSK. Int J Immunopharmacol 11:9–12
Yamaguchi Y, Toge T, Baba N, Kuninobu H, Kegoya Y, Yakayama T, Hattori T (1990) The analysis of lymphocyte surface receptors recognized by wheat germ agglutinin for negative regulation of immune responses in cancer patients. Jpn J Surg 20:51–55
Lanier LL, Loken MR (1984) Human lymphocyte subpopulations identified by using three-color immunofluorescence and flow cytometry analysis: Correlation of Leu-2, Leu-3, Leu-7, Leu-8, and Leu-11 cell surface antigen expression. J Immunol 132:151–156
Perussia B, Fanning V, Trinchieri G (1983) A human NK and K cell subset shares with cytotoxic T cells expression of the antigen recognized by antigen OKT8. J Immunol 131:223–231
Lanier LL, Le AM, Philips JH, Warner NL, Babcock GF (1983) Subpopulations of human natural killer cells defined by expression of the Leu-7(HNK-1) and Leu-15(NK-15) antigens. J Immunol 131:1789–1796
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Toge, T., Yamaguchi, Y. & Sawamura, A. The role of lymphocyte surface binding sites for wheat germ agglutinin in the negative regulation of cancer patients. Surg Today 23, 765–770 (1993). https://doi.org/10.1007/BF00311617
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DOI: https://doi.org/10.1007/BF00311617